Báo cáo khoa học: Biochemical characterization of the native Kv2 1 potassium channel ppt
Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel ppt
... 29 31 i ii Load 3 4 5 7 9 11 1 315 1 719 212 3 Kv2. 1 Load iii 3457 9 11 1 315 1 719 212 3 Kv2. 1 Load iii Protein concentration (A595) * * 25 25 25 Fig. 4. Chromatographic fractionation of native Kv2. 1 complexes. ... extracts Desalting (PD -10 ) I. Mono- Q II. Gel Filtration III. Mono-S Kv2. 1- enrichment (50X) 5.5 X 2 .1 X 4.3 X Kv2. 1 Kv4.2 * Kv1.2 15 0 kDa 10 0 75...
Ngày tải lên: 30/03/2014, 20:20
Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx
... 18 17 14 Pro 15 (4.8) 12 10 21 17 17 11 15 11 Ser 27 (8.7) 36 37 26 31 25 29 30 42 Thr 25 (8 .1) 26 27 16 19 25 22 16 16 Trp 2(0.6 )11 111 2 2 1 Tyr 6 (1. 9)64465 4 9 4 Val 23 (7.4) 17 20 26 17 17 ... 26 17 28 26 24 His 5 (1. 6)54453 5 4 3 Ile 8 (2.6) 14 11 11 13 13 13 12 15 Leu 29 (9.4) 32 31 30 28 35 25 30 21 Lys 14 (4.5) 7 10 6 4 6 12 8 10 Met 3 (1....
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Biochemical characterization of the minimal polyketide synthase domains in the lovastatin nonaketide synthase LovB pot
... USA Fax: +1 310 206 410 7 Tel: +1 310 825 0375 E-mail: yitang@ucla.edu (Received 1 March 2007, revised 29 March 2007, accepted 2 April 2007) doi :10 .11 11/ j .17 42-4658.2007.05 818 .x The biosynthesis of lovastatin ... MALDI-TOF analysis (Observed: 12 225; Expected: 12 2 21; Apo form: 11 8 81) . Appending the CON domain to the ACP domain resulted in a 15 -fold increase in t...
Ngày tải lên: 30/03/2014, 08:20
Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot
... 1 D Æs )1 ) k cat ⁄ K m c (M DCIP )1 Æs )1 ) Ca 6.0 ± 1 108 ± 12 42 ± 12 12 2 ± 13 2 .1 1.9 · 10 3 5.0 · 10 4 1. 7 · 10 3 DNCa 5.6 ± 0.6 11 1 ± 18 65 ± 22 40 ± 4 2.2 2.0 · 10 3 3.4 · 10 4 5.5 · 10 4 a The ... 98 ± 17 84 ± 8 Acetylsalicylic acid 67 ± 12 10 4 ± 11 Alloxan (10 m M)89± 910 3±6 Amytal 57 ± 14 10 3 ± 22 Antimycin A (0.5 m M)53±356±3(1mM) Atova...
Ngày tải lên: 07/03/2014, 12:20
Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... 4 .1 min 1 k 1 = 1 x 10 7 M 1 · min 1 k 1 = 0.9 min 1 k –3 = 0.09 min 1 k 3 = 3.5 x 10 3 M 1 ·min 1 E + P K d P = 69 nMK d S = 90 nM k c = 5.7 min 1 E P Fig. 2. Kinetic scheme for the ... cleavage P1 (RE1) P1 (RE1) P10 (RE3) P10 (RE3) A G 5′ G C 5′ c u c c u c 3′ Ribozyme P1 (RE1) 1P1P (RE1) (RE2) u g a c u A G U G 5′ G C A U 3′ (10 -mer substrate) Step 1 Rib...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... assays for the mAU 0 20 40 60 80 10 0 12 0 14 0 010 20 min 1 4 AB C mAU 0 20 40 60 80 10 0 12 0 14 0 mAU 0 20 40 60 80 10 0 12 0 14 0 0 10 20 min 1 2 3 4 0 10 20 min 1 2 3 4 glutaconyl-CoA glutaconyl- CoA 3-hydroxy- glutaryl-CoA 3-hydroxy- glutaryl- CoA glutaconyl- CoA Fig. ... Mannheim, Windeckstr. 11 0, 6 816 3 Mannheim, Germany Fax: +49 6 212 926420 Tel: +49 6 212 92...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... 2.43 6.7 3 14 3.4 71, 41 4 4.88 11 1.3 71, 3 5 1. 80 21. 4 41, 3 Dimethylallyl diphosphate 1 4.49 6.6 6.6 2 5.48 7 .1 3 13 9.7 41, 42 4(E-methyl) 1. 79 24.7 42, 4 5(Z-methyl) 1. 75 17 .0 41, 4 a Referenced ... kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10 ) are devoid of genes for the biosynthesis of IPP and DMAPP. The...
Ngày tải lên: 19/02/2014, 13:20
Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... 3.4 a 0.045 1. 6 · 10 3 12 7 Ub-K-TAMRA 10 00 10 .9 ± 1. 0 0. 018 1. 6 · 10 3 644 208–560 Ub-AMC 10 0 44.2 ± 3.8 a 0.077 1. 7 · 10 3 11 9 Ub-K-TAMRA 2000 36.8 ± 4.9 a 0.039 1. 1 · 10 3 936 208 11 02 Ub-AMC ... 2+ 567.9 600.4 b10 11 34.7 750.4 b9 10 21. 7 b 11 1247.9 b12 13 76.8 MH 3 3+ -H 3 PO 4 y''6- H 3 PO 4 LE/EL b2 227.2 y'‘8 2+ y8 9 71. 6 873.6 y&apo...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf
... annexin B1 12 3 45 6 7 8 910 12 34567 8 910 kDa A B 15 8 11 6 97.2 66.4 55.6 42.7 36.5 26.6 20.0 kDa 15 8 11 6 97.2 66.4 55.6 42.7 36.5 26.6 20.0 Fig. 2. Purification of native annexin B1. Fractions of the ... element. Experiments in the presence of calcium were carried out with the addition of 5 mm CaCl 2 in the buffer. For investi- gation of the thermal denatura...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... Universita ¨ tsstraße 15 0, 44800 Bochum, Germany Fax: +49 2343 214 785 Tel: +49 234322 417 3 E-mail: chr.herrmann@rub.de (Received 9 October 2009, revised 15 January 2 010 , accepted 19 January 2 010 ) doi :10 .11 11/ j .17 42-4658.2 010 .07586.x The ... (kcalÆmol )1 ) TÆDS (kcalÆmol )1 ) hGBP5a ⁄ b GDP 7.2 ± 0.3 )14 .7 ± 0 .1 )7.7 ± 0.2 GppNHp 2.6 ± 0.3 ) 31. 3 ± 0.4 )23.5 ± 0.6...
Ngày tải lên: 15/03/2014, 10:20