Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc

... LaRonde-LeBlanc et al. Structure and activity of the Rio1 kinase FEBS Journal 272 (2005) 3698–3713 ª 2005 FEBS 3713 Structure and activity of the atypical serine kinase Rio1 Nicole LaRonde-LeBlanc 1 , ... with the uncomplexed protein. Com- parisons of the structure of Rio1 with the previously determined structure of the Rio2 kinase defined...

Ngày tải lên: 30/03/2014, 20:20

... with the gene of the benzaldehyde lyase and for helpful discus- sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... comparison of the relative B-factors along the ThDP molecules shows that the Fig. 4. Structure- based sequence alignment of BAL, BFD and PDC all of which ar...

Ngày tải lên: 07/03/2014, 12:20

... shifts for DMT1-TM4 in TFE and SDS. Ó FEBS 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951 Structure and topology of the transmembrane domain 4 of the divalent metal transporter ... structures of DMT1-TM4 in both SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig. 3. The quality of t...

Ngày tải lên: 16/03/2014, 16:20

... in the mutant. First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... catalysis. The alignment of the small CMLEs from different members of the Rhizobiales with these sequences demonstrated that in A. radiobacter S2 (and the other mem...

Ngày tải lên: 30/03/2014, 10:20

... Hence, the maintenance of a condensed chromatin structure throughout the entire body of a gene may rely on the combined actions of Set3 at the 5¢ end and Set2 at the 3¢ end. In yeast the cycle of ... C (1989) The limits of the DNase I-sensitive domain of the human apolipoprotein B gene coincide with the locations of chromosomal anchorage loops and define...

Ngày tải lên: 14/02/2014, 18:20

... N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionic interactions established between Lys11/Tyr13 of ... Like prophytepsin, the precursor form of cyprosin was processed in different isoforms by the excision of the prosegment and of most of the PSI [2...

Ngày tải lên: 19/02/2014, 12:20

... in which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja- cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... single- stranded conformation across one face of the KH domain, between the a1-helix and the a2-helix and GXXG on the ‘left’, and the b2-sheet and the va...

Ngày tải lên: 07/03/2014, 05:20

... present study, comparison of GlcNAc kinase with a model of the three-dimensional structure of glucokinase provides the first picture of the possible structure of the enzyme. Furthermore, two active ... consists of the phosphate1 subdomain and the predicted a-helix I. The other lobe consists of the phos- phate2 and the adenosine subdomains, the predic...

Ngày tải lên: 08/03/2014, 10:20

... estimation of the hydrodynamic radii of HAMLET (R h = 26.9 A ˚ ), which is intermediate of the hydrodynamic radii of the acidic molten globule state of a-lactalbumin (R h = 20.9 A ˚ ) and the theoretically ... fully understood. The active group of the matrix, the DEAE group, is posi- tively charged and might therefore bind negative mole- cules. At pH 8.5 (the pH...

Ngày tải lên: 29/03/2014, 21:20

... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil- ity of the residual activity of the majority of p53 mis- sense mutants. The biological activity of mutant ... powerful method, and several algorithms have been developed recently [4–7]. The major drawback of these analyses is the lack of information regarding the...

Ngày tải lên: 18/02/2014, 11:20