Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp PCC 7119 potx
Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx
... 2005)doi:10.1111/j.1742-4658.2005.04747.xWe investigated the role of electrostatic charges at positions D72 and K8 in the function and structural stability of cytochrome c6 from Nostoc sp. PCC 7119 (cyt c6). A series of mutant ... crossingregion of the helices, and the Nxatom of K8 is foundat a distance of 6.2 A˚ from the nearest side-chain oxy-gen of D72. Taking the functionally and structurally interestingmutant D72K ... interactions play a critical role in guiding and stabilizing functional protein–pro-tein interactions [6], as well as in the structural stability of proteins [23–25].Inspection of a preliminary...
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Báo cáo khoa học: "A Latent Topic Extracting Method based on Events in a Document and its Application" pot
... werecognized the necessity of such type of an Event byinvestigating the extracted pairs of words and com-paring them with the content of the target document in preliminary experiments, and could ... that the top-ics onto the words in the articles were specific tosome extent; in other words, the words often used in a particular field are often used in newspaper ar-ticles, therefore, we think ... compare the performance of the above measures when using eventLDA.4.2 Experimental SettingsAs for the documents used in the experiment, we usea set of data including users’ reviews and their...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... control the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering ... distribution,making them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without ... signalling to activate the kinases FAK and Src [11], although mutation of the RGD-motif in CagL had no defect in T4SS functionssuch as phosphorylation of injected CagA during infec-tion in another...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... withglycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible datawas obtained from injections of the metal into a solution of the protein. Typically ... proteins they probably formpart of the copper binding sites in this protein.Although there are four histidines in the C-terminal,another histidine is present at position 18, and given the folded ... research is thatunderstanding what causes the loss of appropriatemetal binding might be crucial for the understanding of the role of amyloidogenic proteins in a number of neurodegenerative disorders.Experimental...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... Takentogether, our combined calorimetric and protein chemicalapproach leads to a more detailed understanding of the role of K22 and R120 with respect to fosfomycin binding as wellas the ligand-induced ... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K doublemutant protein point toward a central role of arginine ... with the K22V and K22E mutantproteins. This was interpreted as a lack of fosfomycinbinding to these mutant proteins [12]. Binding of fosfomycinto either wild-type MurA or the K22 mutant proteins...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the V. spinosum genome. ... whether the C-terminal domain in the plant and fungal enzymes has a purely inhibitory function and ⁄ or a role in membrane binding is unclearat present. With regard to V. spinosum pro-tyrosinase,sequence ... structure in the transcript, indica-tive of a site of transcription termination. The presence of these features may indicate that the tyrosinase geneis part of an operon.As stated in the Introduction,...
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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt
... apo-DptF. In vivo 4¢-phosphopantetheinylation of apo-LipD was car-ried out in BL21(DE3)-pRep4-gsp. The transfer assays toholo-LipD and holo-AcpK and determination of the kineticparameters for the ... with the promiscu-ous 4¢-phosphopantetheinyl transferase Sfp from Bacil-lus subtilis and fluoresceinyl CoA [23]. The successful4¢-phosphopantetheinylation of DptF was monitored by the in- gel ... –Fig. 4. In vitro phosphopantetheinylation of apo-DptF and apo-AcpK. Coomassie blue-stained SDS–PAGE gel (left) and in- gel fluo-rescence (right) of the fluoresceinyl-ACP. (+) indicates the reactionwith...
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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot
... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linkedgalactosyl residues, and a terminal-linked N-acetylglu-cosaminosyl ... [M-H]–ions. The source of the ions was as indicated in the structure shown in the inset. The m ⁄ z 917.4 and 797.4 are due to sodiated and dehydratedforms, respectively, of the m ⁄ z 895.4 ... construction of the lgt3 mutant in M. catarrhalisO35E. Large arrows represent the direction of transcription; the site of the transposon insertion identified in the O35E is denotedas EN:TN, and the location...
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Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
... (PLCb)2 and PLCb3, resulting in hydrolysis of PtdIns(4,5)P2into diacylglycerol and InsP3[7,8]. The dissociation of the Gbc subunit can also result in activation of phosphatidylinositol 3-kinase ... whichcatalyzes the production of ROS and results in efficientkilling of invading pathogens [5]. The effect of the calmodulininhibitor W7 on fMLP induced ROS production wasinvestigated and compared with the ... counteracts the calcineurininduced affinity switch of a5b1integrin in CHO cells,suggesting also a role for Ca2+/calmodulin-dependentmolecules in regulating the affinity state of integrins onneutrophils...
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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
... of the active site was better in the protein bear-ing the N2 domain than in the protein bearing the Ncdomain. Thus, the accessibility of the active site in the N2 proteins was independent of ... of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL wereinvolved in the inactivation of the protein at the water–triglyceride interface. The inactivation of ... salts and colipase depen-dence. In order to investigate the role of the two domains of PLRP2 in the function of the protein, two chimeric proteins were designed by swapping the N and C structural...
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