Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp PCC 7119 potx

... 2005) doi:10.1111/j.1742-4658.2005.04747.x We investigated the role of electrostatic charges at positions D72 and K8 in the function and structural stability of cytochrome c 6 from Nostoc sp. PCC 7119 (cyt c 6 ). A series of mutant ... crossing region of the helices, and the N x atom of K8 is found at a distance of 6.2 A ˚ from the ne...

Ngày tải lên: 30/03/2014, 20:20

... we recognized the necessity of such type of an Event by investigating the extracted pairs of words and com- paring them with the content of the target document in preliminary experiments, and could ... that the top- ics onto the words in the articles were specific to some extent; in other words, the words often used in a particular field are often used in n...

Ngày tải lên: 23/03/2014, 16:20

... control the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial for triggering ... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-ca...

Ngày tải lên: 14/02/2014, 19:20

... with glycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible data was obtained from injections of the metal into a solution of the protein. Typically ... proteins they probably form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another histidine is present a...

Ngày tải lên: 19/02/2014, 06:20

... Taken together, our combined calorimetric and protein chemical approach leads to a more detailed understanding of the role of K22 and R120 with respect to fosfomycin binding as well as the ligand-induced ... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K double mutant protein poi...

Ngày tải lên: 19/02/2014, 13:20

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the V. spinosum genome. ... whether the C-terminal domain in the plant and fungal enzymes ha...

Ngày tải lên: 06/03/2014, 11:20

... apo-DptF. In vivo 4¢-phosphopantetheinylation of apo-LipD was car- ried out in BL21(DE3)-pRep4-gsp. The transfer assays to holo-LipD and holo-AcpK and determination of the kinetic parameters for the ... with the promiscu- ous 4¢-phosphopantetheinyl transferase Sfp from Bacil- lus subtilis and fluoresceinyl CoA [23]. The successful 4¢-phosphopantetheinylation of DptF...

Ngày tải lên: 07/03/2014, 04:20

... that the OSs from the wild-type strain consisted of terminal-, 2-, 4-, and 3,4,6-linked glucosyl residues, terminal- and 4-linked galactosyl residues, and a terminal-linked N-acetylglu- cosaminosyl ... [M-H] – ions. The source of the ions was as indicated in the structure shown in the inset. The m ⁄ z 917.4 and 797.4 are due to sodiated and dehydrated forms,...

Ngày tải lên: 07/03/2014, 05:20

... (PLCb)2 and PLCb3, resulting in hydrolysis of PtdIns(4,5)P 2 into diacylglycerol and InsP 3 [7,8]. The dissociation of the Gbc subunit can also result in activation of phosphatidylinositol 3-kinase ... which catalyzes the production of ROS and results in efficient killing of invading pathogens [5]. The effect of the calmodulin inhibitor W7 on fMLP induced ROS...

Ngày tải lên: 08/03/2014, 10:20

... of the active site was better in the protein bear- ing the N2 domain than in the protein bearing the Nc domain. Thus, the accessibility of the active site in the N2 proteins was independent of ... of the water–lipid interface. Also, both the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–t...

Ngày tải lên: 16/03/2014, 06:20