Báo cáo khoa học: Inhibition of the D-alanine:D-alanyl carrier protein ligase from Bacillus subtilis increases the bacterium’s susceptibility to antibiotics that target the cell wall potx

... 2005 FEBS 3003 Inhibition of the D-alanine:D-alanyl carrier protein ligase from Bacillus subtilis increases the bacterium’s susceptibility to antibiotics that target the cell wall Juergen J. ... inhibitor. To test if the inhibitor 5 was able to penetrate the cell wall to reach its target DltA, we investigated chan- ges in the growth...

Ngày tải lên: 30/03/2014, 16:20

... folding of the protein, or a charged residue close to the [Fe–S] center that has a pK a close to that of the sample at pH 8 so that it is only charged in a fraction of the samples (about 50%). The ... suggesting that the integrity of the [Fe–S] cluster is important for the binding of iminoaspartate to NadA. Conclusions In the past few years there has...

Ngày tải lên: 30/03/2014, 02:20

... health. The accumulation of a threshold level of Hsp70 sig- nals the cessation of Hsp mRNA transcription and the restoration of the normal rate of protein synthesis. This has been termed ‘autoregulation’ ... Brilliant Blue staining of the gels after electrophoresis). Labeled proteins were detected by autoradiography. The basic region of the gel is to the righ...

Ngày tải lên: 30/03/2014, 20:20

... operating at various steps in the cytotoxic activity of the drug, from a decrease of drug accumulation in the cell to abrogation of apoptosis induced by the chemical substance. Often several different ... determining the sensitivity of tumor cells to chemo- therapy and is linked to activation of the phosphatidylinositol-3-kinase/Akt (PI3K/Akt) pathway. In the...

Ngày tải lên: 07/03/2014, 03:20

... interaction then results in opening of these channels [9,10]. The mechanism of CaM-mediated closure of hEAG1 channels is far from understood mainly because of the lack of structural information on the ... we assured that addition of 1 lm CaM to the Ca 2+ -containing buffer did not alter the concentration of free Ca 2+ ions. For application of His-tagged hCaM,...

Ngày tải lên: 07/03/2014, 12:20

... F). These data indicate that elevation of intracellular cAMP and subsequent activation of PKA lead to the inhibition of NF-jB activity. To further examine the effect of PKA on NF-jB activity, we ... common feature of the regulation of the NF-jB family is their sequestration in the cytoplasm as inactive complexes with a class of inhibitory molecules known as IjB...

Ngày tải lên: 08/03/2014, 10:20

... suggest that the cytosolic calcium concentration ([Ca 2+ ] c ) regulates both the utilization of ATP in the contractile process, as well as the mitochondrial production of ATP, by increasing the mitochondrial ... much 103 Ru 360 com- pared to the enriched mitochondrial fraction (2.3 ± 0.6 pmol of 103 Ru 360 Æmg )1 of protein vs. 1.2 ± 0.15 pmol 103 Ru 360 Æmg )1 of...

Ngày tải lên: 16/03/2014, 22:20

... after removal of excess DA, the DA-modified protein (mixture of monomer and oligomer), also retains the cytotoxicity, supporting the hypothesis that the cyto- toxicity primarily arises from the reaction ... or dimers. The DA quinoprotein retains the same cytotoxicity as the intact a-Syn, suggest- ing that the oligomeric intermediates are the major elements that...

Ngày tải lên: 23/03/2014, 15:20

... inhibitors of methionine synthase. Our results indicate that the potency of the tested compounds is related to a planar region of the inhibitor that can be positioned in the centre of the active ... enzyme. The anaesthetic gas N 2 O is possibly the only selective inhibitor of MetS reported to date, its action mediated through the oxidation of the cobala-...

Ngày tải lên: 19/02/2014, 05:20

... in the separ- ation of the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of ... inhibit the attach- ment of the CBPs to the cell wall at lower concen- trations may allow the development of new antimicrobial therapies [27] to address...

Ngày tải lên: 19/02/2014, 05:20