Báo cáo khoa học: A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system potx

... K, Murata T, Tanaka M, Tobe T, Iida T, Takami H, Honda T, Sasakawa C, Ogasawara N, Yasunaga T, Kuhara S, Shiba T, Hattori M & Shinagawa H (2001) Complete genome sequence of enterohemorrhagic Escherichia ... However, ANS bind- ing experiments indicate that a conformational change occurred upon decreasing pH. The characteristics of this conformational change are consistent...

Ngày tải lên: 30/03/2014, 16:20

... topography of the interacting helical faces of subunits a and c from the I. tartaricus ATP synthase has been mapped. According to these data, the essential stator arginine (aR226) is located between ... sub- unit a and the c-ring were made several years ago by Fillingame et al. They presented an elaborate study on the interacting helical faces of subunits a and c of E...

Ngày tải lên: 16/03/2014, 06:20

... domains D and E, they do not normally contain an N-domain, and are thus five-domain proteins possessing the catalytic (b /a) 8 -barrel (domain A) and the four domains B, C, D and E. In the case of ... illustrating the arrangement of all domains in the five-domain members of the GH 13, can be taken as an approximation of the first three domains in the four-domain m...

Ngày tải lên: 08/03/2014, 08:20

... documentation of ACE 2005 Multilingual Training Data V3.0. Since the final release of the ACE training corpus only contains the final adjudicated annotations, in which all the traces of the ... entities that appear in the same sentence but are not tagged as any of the types. Alternatively, one could apply a hierarchical learning strategy, which trains two c...

Ngày tải lên: 24/03/2014, 03:20

... linker between the SH2 and kinase domains at the back of the small lobe, preventing the formation of a produc- tive catalytic cleft [29]. Thus, these interactions clamp the kinase domain in an inactive ... [12]. An increase in the stimu- lus level can gradually increase Src activity, or transfer the system into a bistable domain, where a further increase in th...

Ngày tải lên: 18/02/2014, 11:20

... the artifact may be caused by using a plant translation system, in which mammalian mRNA was inappropriately translated. The transient expression assay indicated that M33S and isoform 1 proteins were ... FEBS F 1a, 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT GCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCC GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢ (Fig. 2A)...

Ngày tải lên: 19/02/2014, 06:20

... 4¢-phosphopantothenate is not commercially available, it was synthesized enzymatically in situ by adding pantothenate kinase, His-CoaA, D -pantothenate and ATP to the His-CoaB assay mixtures [5]. Therefore, 0.9 ... b-alanine. Molecular analysis of the conditional lethality of the dfp-707 mutant revealed a single point mutation within the N-terminal CoaC domain of Dfp [4]. I...

Ngày tải lên: 19/02/2014, 12:20

... as adjacency pairs, then knowing the DA category of the B part and of all potential A parts should help in finding the most meaningful dialog act tag among all potential A parts; for example, the ... the problem as a binary classification problem where 3 The approach is generally called re-ranking in cases where candidates are assigned an initial rank beforehand. each...

Ngày tải lên: 20/02/2014, 16:20

... CCTTTGAGCACATTTCGGCAA-3¢ P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 289 5¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢ b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 665 5¢-3222 AGCCATGCCAAATGTCTCAT-3¢ Fig. 1. Dose-related ... AGGTGCTTTGCCAGATATGACT-3¢ 432 5¢-802 CTCTTTGATGACAGAAGTGCCT-3¢ Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 365 5¢-450 AAAAATGTTGCTGCCCTG-3¢ Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢...

Ngày tải lên: 21/02/2014, 03:20

... residues in the N-terminal region are separated by five amino acids, and by a similar molecular weight of approximately 17 kDa. The discovery of Prxs in archaea, defined together with cyanobacteria, the ... in damage to the main biomole- cules and membranes and essential metabolic functions [1]; to maintain a low intracellular ROS level, cells are equipped with an array o...

Ngày tải lên: 07/03/2014, 05:20