Báo cáo khoa học: Formation of nucleoprotein RecA ﬁlament on

Báo cáo khoa học: Formation of nucleoprotein RecA ﬁlament on single-stranded DNA Analysis by stepwise increase in ligand complexity potx

... 2745 Formation of nucleoprotein RecA ﬁlament on single-stranded DNA Analysis by stepwise increase in ligand complexity Irina P. Bugreeva, Dmitry V. Bugreev and Georgy A. Nevinsky Institute of Chemical ... than dsDNA. After strand exchange, the second RecA DNA binding site binds the displaced strand following new DNA duplex formation [12]. Binding of...

Ngày tải lên : 30/03/2014, 16:20

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Báo cáo khoa học: Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin pptx

... M (2004) Kinetics and binding sites for interaction of the prefoldin with a group II chaperonin: contiguous non-native substrate and chaperonin binding sites in the archaeal prefoldin. J Biol ... Chauhan VP (1998) Binding of amyloid beta-protein to intracellular brain proteins in rat and human. Neuro- chem Res 23, 1277–1282. Formation of amyloid beta oligomers by prefoldin M....

Ngày tải lên : 07/03/2014, 04:20

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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... glucan synthesis. Abbreviations DP, degree of polymerization; GBd, glucan-binding domain; GS, glucan-binding domain-deﬁcient glucosyltransferase-I; GSd, glucansucrase domain; GSGB, glucosyltransferase-I containing a ... of dextran-independent insoluble glucan formation and examined the enzyme kinetic properties of the nigero- oligosaccharide acceptor reaction by using GBd-deﬁcient G...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... expression of any gene of interest. Gene silencing by RNA interference (RNAi) is mediated by endogenous proteins, resulting in tar- get mRNA cleavage or translational inhibition [1]. In the cytoplasm ... compilation ª 2009 FEBS 6579 of TRBP in this complex. Binding reactions performed in extracts after TRBP silencing showed a concomitant reduction in binding at the expecte...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... that the conformational changes of C-LytA upon binding of these alkaloids are different to those induced by choline, suggesting a different mode of binding. In vitro inhibition assays of three ... cultures to increasing concentrations of choline abolishes daughter cell separation at the end of cell division, causing the formation of long chains of cells with only s...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

... critical r ole in the regulation of cell proliferation, including SMC, by inhibiting signalling mol- ecules [ 45–47]. Caveolin-3 is the main isoform in muscle cells, including SMC [30]. Caveolin-1 and ... of MAPK [12,43,44], the inhibition of MAPK by the incorporation of n-3 PUFAs could alter the phosphorylation of these transcription factors, affecting their binding to DNA...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Cleavage of nonphenolic b-1 diarylpropane lignin model dimers by manganese peroxidase from Phanerochaete chrysosporium Evidence for a hydrogen abstraction mechanism docx

... was conducted in the absence of Tween 80. In contrast to the oxidation of this substrate by lignin peroxidase (LiP), oxidation of substrates by MnP did not proceed under anaerobic conditions. ... (polyoxyethylenesorbitan monooleate) (0.1%). LiP reactions were conducted at 28 °Cfor5min in 1 mL of 20 m M succinate, pH 3.0, containing the substrate (180 l M ) and enzyme (5 lg)....

Ngày tải lên : 20/02/2014, 23:20

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Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf

... main phases – initiation, elongation, termination, and recy- cling. Initiation is the rate-limiting step [1]. This phase is mediated by initiation factor 1 (IF1), initiation factor 2, and initiation ... Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin. FEBS J 277, 2428–2439. 12 Milon P, Konevega AL, Gualerzi CO & Rodni...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx

... C-terminal domain of Ca 2+ -free CaM, i.e. apoCaM. The activation of these channels is mediated by conformational changes in the CaM ⁄ SK2 complex induced by binding of at least one Ca 2+ ion ... CaM. Assaying CaM binding to GST-fusion proteins of the C-terminal domain of hEAG1 channels, a binding site was locali- zed within amino-acids 673–770. This stretch of resi- due...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc

... combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more ﬂexible b-domain. In addition to the already described differences in ﬂexibility of the ... metallothioneins. This points to an hitherto unknown mechanism or compound(s) being involved in this interaction in living cells and information about this additional fac...

Ngày tải lên : 07/03/2014, 15:20

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