Báo cáo khoa học: Crystal structures of Aedes aegypti kynurenine aminotransferase potx

... Crystal structures of Aedes aegypti kynurenine aminotransferase Qian Han 1, *, Yi Gui Gao 1,2, *, Howard Robinson 3 , Haizhen Ding 1 , Scott Wilson 2 and Jianyong Li 1 1 Department of Pathobiology, ... 2005) doi:10.1111/j.1742-4658.2005.04643.x Aedes aegypti kynurenine aminotransferase (AeKAT) catalyzes the irrevers- ible transamination of kynurenine to kynurenic a...

Ngày tải lên: 30/03/2014, 16:20

... domain of TtAK contributes not only to catalytic regulation, but also the thermostability of TtAK. Comparison of the crystal structures of TtAKb and CgAKb was expected to elucidate the mechanism of ... AK, with a dif- ference in denaturation temperature (T m )of4 0°C. Comparison of the crystal structures of TtAKb and the regulatory subunit of C. glutamicum AK showed t...

Ngày tải lên: 18/02/2014, 08:20

... These structures provide insight into the key features of PDH specificity and mode of inhibition of the enzyme. Results and Discussion Structural analysis and model quality Crystal structures of ... comparing the cofactor domains of the OaPDH and TbPDH structures [13]. The superposition of sub- unit A of complex I onto subunits A, B, C of complex II and III yield rmsd-v...

Ngày tải lên: 19/02/2014, 05:20

... carbon of fructose. The leaving group is carboxylate of Asp54. Dimerization The asymmetric units of the crystals of both the apo and complexed form of the enzyme consist of dimers with noncrystallographic ... level of 99.9%. Other parameters were also obtained: hydrodynamic radius of 16.78 A ˚ , average radius of 34.02 A ˚ and ratio of the shortest to the longest axis of...

Ngày tải lên: 06/03/2014, 00:21

... the crystal structures of the NiV two-helix and HeV two-helix to 2.2 A ˚ resolution, thus confirming the formation of a six-helix bundle. These structures also show the typical characteristics of ... Rao Z & Gao GF (2003) Crystallization and preliminary X-ray crystallographic analysis of the trimer core from measles virus fusion protein. Acta Crystallogr D Biol Crystallogr 59...

Ngày tải lên: 08/03/2014, 08:20

... the NMR structure of SUMO-1 and the crystal structure of yeast A CD B Fig. 2. Photographs and electron density maps of the SUMO-2 crystals. Shown in (A) and (B) are two different crystal forms I ... possible for the Lys11 of one SUMO-2 molecule to form an isopeptide bond with the Gly93 of another. The crystal structures of diubiquitin and tetraubiquitin showed some alternat...

Ngày tải lên: 16/03/2014, 18:20

... phenotype. Previously, the crystal structure of the HIV protease in complex with indinavir was reported at a resolution of 2.0 A ˚ [17,18]. The crystal structures of several multiple mutant drug-resistant ... resolution crystal structures. The O4 atom of indinavir formed hydrogen bond interactions with the amide and OD2 of Asp29¢,and interacted via water OB with the carbony...

Ngày tải lên: 23/03/2014, 12:20

... sample, crystals were obtained under the same condition as that of the WtDSD crystals. The quality of these crystals was bet- ter than the WtDSD crystals or WtDSD crystals obtained in the presence of ... Bharath et al. Crystal structure of D-serine deaminase FEBS Journal 278 (2011) 2879–2891 ª 2011 The Authors Journal compilation ª 2011 FEBS 2891 Crystal structures of open and...

Ngày tải lên: 28/03/2014, 22:20

... deter- mined the crystal structures of two enzymes, TVA I [4,5] and TVA II [6,7], both of which hydrolyze CDs and a polysaccharide pullulan. We have also deter- mined the crystal structure of a solute-binding ... CymA of Klebsiella oxytoca outer membrane: binding of cyclo- dextrins and study of the current noise of the open channel. Biophys J 85, 876–885. 43 Otwinowski Z &...

Ngày tải lên: 29/03/2014, 23:20

... incubated withafixedamountofAMA(3l M ) and increasing concentrations of odorants (0.39–50 l M ). The chasing of AMA bound to OBP was followed a s a decay of t he emission of the fluorescence intensity ... and the concentration of AMA. Crystallization, data collection and refinement of bovine OBP Except for the bOBP–UND complex, bOBP crystals were obtained by m icrodialysis of a 1 0...

Ngày tải lên: 30/03/2014, 15:20