Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein Sabine Andre´1, Shuji Kojima2, Ingo Prahl3, Martin Lensch1, Carlo Unverzagt3 and Hans-Joachim ... 879–886. Extended LEC14-type N-glycan as lectin ligand S. Andre´et al.1998 FEBS Journal 272 (2005) 1986–1998 ª 2005 FEBS Introduction of extended LEC14-type branching into core-fucosylated biantennary ... (EAT) cells hadbeen injected subcutaneously into the right rear leg of maleddY mice of the age of 7 weeks for tumor inoculation. OnS. Andre´et al. Extended LEC14-type N-glycan as lectin ligandFEBS...
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Báo cáo khoa học: "Introduction of a new paraphrase generation tool based on Monte-Carlo sampling" potx
... func-tion and the value of each (state, action) pair of the episode is updated. Then, the algorithm computesan other episode with the new values.Periodically, the sampling step is stopped and the ... Callison-Burch,2005). The question asked to the human evaluator for the syntactic task is: Is the following sentence ingood French? The question asked to the humanevaluator for the semantic task is: Do the followingtwo ... a variation of the UCT algorithm for paraphrase generation named MCPG for Monte-Carlo based Paraphrase Generation. The main part of the algorithm is the samplingstep. An episode of this step...
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Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... increases the ATPase activity of these proteins [26]. To test whether the ATPase activity of IRP-1 is also enhanced in the presence of IRE, we measured hydrolysis of [32P]ATP[cP] in the presence of ... 1) and ⁄ or that ATP is required for iron-mediated regulation of IRP-1 binding activity. On the other hand, a Kd of 86 lm argues against directregulation of IRP-1 binding by ATP. Total cellularATP ... Site-directedmutational analysis for the ATP binding of DnaA pro-tein. Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP -binding domain of DnaA protein in vitro and in vivo. J Biol...
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Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... an hCaM band is only seen in the presence of Ca2+(lanes 5, 6 and 9, 10). In the absence of Ca2+(lanes 3, 4 and 7, 8) and for the GST control (lanes 1 and 2) there was no retention of hCaM ... 9 nm for the wild-type peptide); EF-hand mutations 3 and 4 abolished binding (KD>5lm for hCaM-EF34 and hCaM-EF1234). None of the CaM constructs bound to the peptide in the absence of Ca2+. ... weight ratio of the fluorescentlylabeled molecule and the formed binding complex wasABFig. 5. Functional impact of mutations in the N-terminal CaM-bind-ing site and in the EF-hand lobes of hCaM....
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Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt
... barrier in epithelialcells. The entry of EPEC into cells is a complexprocess, and the underlying molecular and cellularmechanisms of EPEC-induced disruption of the epithelial barrier are not ... limited to the cell cell interfaces, and the staining is continuous and uniform. After infection with EPEC, the distribution of occludin and ZO-1 wassignificantly altered and breaks in occludin and ... unin-fected cells, occludin and ZO-1 were found to beprimarily localized to the cell membrane of the TJ.Determination of the localization of these two proteinsshowed a continuous and uniform distribution...
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Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
... One was the identification of the minimal essential region required for the interaction with the middle domain, which mediates the dimerization of HSP90, and the other, the identification of the minimal ... Interaction between the middle and C-terminal domains of GRP94. The bacterial two-hybrid system was used to evaluate the binding activity. The value of the combination of the middle and the C-terminal ... roles of the C-terminal domain of HSP90 (Eur. J. Biochem. 270) 151therefore, it is still ambiguous whether the regions, especially the region responsible for dimer formation and that for client binding, ...
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Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-affinity thrombopoietin antagonists and agonists pot
... con-formation. Structural data on the main chain confor-mation of the transplanted loop sequences within the context of the respective CK scaffold in free form and bound to the c-Mpl extracellular ... loca-tion within the CK scaffold and their main chain con-formation. Whereas in EETI-II the distance between the Ca atoms of CI and CIV is 11.8 A˚ and an exten-ded loop is formed by six intervening ... chimera comprising the ectod-omain of c-Mpl fused to the cytoplasmic part of the IL-4Ra chain. The chimeric receptor was transientlytransfected into Ba ⁄ F3 cells along with the luciferasereporter...
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Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... in the cell culture. The presence of these single fluorophore-expressingcells were essential for the FRET assay because the fluorescence of these cells served as references for the subtraction of ... cell type, the quality of the DNA, the quantity of DNA taken up by the cells, the cytotoxicity of the transfection reagents, and the condition of the cells. For example, low transfection efficiency ... productTable 1. The probability of formation of various complexes with refer-ence to the ratios between molecules A and B. The Hardy–Weinberg lawwas used as the mathematical model for calculating the frequency...
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Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot
... controlled by the methyla-tion-free promoter. One of the common features of these plants free from S-PTGS is the attenuatedexpression of the transgene, and therefore the thresh-old theory has ... plants, and hybridized with a probe for the CaMV 35S promoter. The lengths of the observed bands are given on the left. The dot at 1228 bpin the left panel is a hybridization artifact. The 893-bp ... vitro-culturedcells [32]. Expression of the transgene is transcription-ally silenced by dense methylation of the promotersequences, and siRNAs for the nptII gene disappear.When the expression of the...
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Báo cáo khoa học: "Framework of Semantic Role Assignment based on Extended Lexical Conceptual Structure: Comparison with VerbNet and FrameNet" ppt
... predicates and their semanticfunctions.ure 1 represents the action changing the state of j. The inner structure of the second argument of gorepresents the path of the change. The overall definition of ... SourceTheme (162) ThemeTheme1 (13), Theme2 (13) Both of the two is Theme ∨ Theme1 is Theme and Theme2 is StateTopic (18) Theme ∧ selPref(knowledge ∨ infromation)Table 7: Relationship of roles ... struc-ture. For example, we show an LCS structure and a frame in VerbNet with regard to the verb“buy” in Figure 3. The LCS structure consists of four formulae. The first one is the main for- mula and the...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP