Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein Sabine Andre ´ 1 , Shuji Kojima 2 , Ingo Prahl 3 , Martin Lensch 1 , Carlo Unverzagt 3 and Hans-Joachim ... 879–886. Extended LEC14-type N-glycan as lectin ligand S. Andre ´ et al. 1998 FEBS Journal 272 (2005) 1986–1998 ª 2005 FEBS Introduction of e...
Ngày tải lên: 30/03/2014, 16:20
Báo cáo khoa học: "Introduction of a new paraphrase generation tool based on Monte-Carlo sampling" potx
... func- tion and the value of each (state, action) pair of the episode is updated. Then, the algorithm computes an other episode with the new values. Periodically, the sampling step is stopped and the ... Callison-Burch, 2005). The question asked to the human evaluator for the syntactic task is: Is the following sentence in good French? The question asked to the...
Ngày tải lên: 17/03/2014, 02:20
Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... increases the ATPase activity of these proteins [26]. To test whether the ATPase activity of IRP-1 is also enhanced in the presence of IRE, we measured hydrolysis of [ 32 P]ATP[cP] in the presence of ... 1) and ⁄ or that ATP is required for iron-mediated regulation of IRP-1 binding activity. On the other hand, a K d of 86 lm argues against direct regulation of...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx
... an hCaM band is only seen in the presence of Ca 2+ (lanes 5, 6 and 9, 10). In the absence of Ca 2+ (lanes 3, 4 and 7, 8) and for the GST control (lanes 1 and 2) there was no retention of hCaM ... 9 nm for the wild- type peptide); EF-hand mutations 3 and 4 abolished binding (K D >5lm for hCaM-EF34 and hCaM- EF1234). None of the CaM constructs bound to...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt
... barrier in epithelial cells. The entry of EPEC into cells is a complex process, and the underlying molecular and cellular mechanisms of EPEC-induced disruption of the epithelial barrier are not ... limited to the cell cell interfaces, and the staining is continuous and uniform. After infection with EPEC, the distribution of occludin and ZO-1 was significantly...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx
... One was the identification of the minimal essential region required for the interaction with the middle domain, which mediates the dimerization of HSP90, and the other, the identification of the minimal ... Interaction between the middle and C-terminal domains of GRP94. The bacterial two-hybrid system was used to evaluate the binding activity. The value...
Ngày tải lên: 23/03/2014, 20:22
Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-affinity thrombopoietin antagonists and agonists pot
... con- formation. Structural data on the main chain confor- mation of the transplanted loop sequences within the context of the respective CK scaffold in free form and bound to the c-Mpl extracellular ... loca- tion within the CK scaffold and their main chain con- formation. Whereas in EETI-II the distance between the Ca atoms of CI and CIV is 11.8 A ˚ and an exten...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Development of a baculovirus-based fluorescence resonance energy transfer assay for measuring protein–protein interaction potx
... in the cell culture. The presence of these single fluorophore-expressing cells were essential for the FRET assay because the fluorescence of these cells served as references for the subtraction of ... cell type, the quality of the DNA, the quantity of DNA taken up by the cells, the cytotoxicity of the transfection reagents, and the condition of t...
Ngày tải lên: 30/03/2014, 20:20
Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot
... controlled by the methyla- tion-free promoter. One of the common features of these plants free from S-PTGS is the attenuated expression of the transgene, and therefore the thresh- old theory has ... plants, and hybridized with a probe for the CaMV 35S promoter. The lengths of the observed bands are given on the left. The dot at 1228 bp in the left panel is...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: "Framework of Semantic Role Assignment based on Extended Lexical Conceptual Structure: Comparison with VerbNet and FrameNet" ppt
... predicates and their semantic functions. ure 1 represents the action changing the state of j. The inner structure of the second argument of go represents the path of the change. The overall definition of ... Source Theme (162) Theme Theme1 (13), Theme2 (13) Both of the two is Theme ∨ Theme1 is Theme and Theme2 is State Topic (18) Theme ∧ selPref(knowledge ∨ infr...
Ngày tải lên: 24/03/2014, 03:20