Báo cáo khoa học: Hyperthermal stability of neuroglobin and

Báo cáo khoa học: Hyperthermal stability of neuroglobin and cytoglobin doc

... T m . The enthalpy of denaturation DH m of Ngb and horse heart Mb was 72 and 110 kcalÆmol )1 , respectively. Cytoglobin and the globin of Drosophila have lower values of DH m , 60 and 53 kcalÆmol )1 , respectively. ... Ngb had negative bands at 208 and 222 nm (Fig. 1), as expected for a high percentage of alpha helix. Analysis of the secondary structure of Ngb gave 7...

Ngày tải lên : 30/03/2014, 16:20

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Báo cáo khoa học: Conformational stability of neuroglobin helix F – possible effects on the folding pathway within the globin family potx

... Alessandra Corazza 1 , Paolo Viglino 1 , Luc Moens 3 , Gennaro Esposito 1 and Angelo Fontana 2 1 Department of Biomedical Sciences and Technologies and MATI Centre of Excellence, University of ... structural properties of helices F in the two proteins, we analyzed pep- tides encompassing helix F of neuroglobin and myoglobin in the wild-type and mutant forms. NMR and CD...

Ngày tải lên : 30/03/2014, 01:20

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Báo cáo khoa học: Ten years of predictions…and counting docx

... USA) and Anna Tramontano (University of Rome, Italy) with the invaluable help of Andriy Kryshtafovych (Lawrence Livermore National Laboratory) and Volker Eyrich (Columbia University). The goals of ... REVIEW ARTICLE Ten years of predictions … and counting Domenico Cozzetto 1 , Adele Di Matteo 1 and Anna Tramontano 2 1 Department of Biochemical Sciences, University of Rome...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological signiﬁcance docx

... amino acid sequences of Mb and Ngb and the comparison of A B C D Fig. 2. CD characterization of Ngb and Mb at neutral and acidic pH. (A) Far-UV CD spectra of human holoNgb and apoNgb dissolved ... University of Padua, Italy 2 Department of Biochemistry, University of Antwerp, Belgium 3 Department of Zoophysiology, University of Aarhus, Denmark Introduction Neurogl...

Ngày tải lên : 07/03/2014, 02:20

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Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... melting curves of wild-type and mutants of SNase. DSC curves of ﬁve proteins [wild-type (WT), W140A, E140O, W140O and K133A]. The curves of W140A and W140O are nearly linear in terms of intensity. ... 3961 of the wild-type protein and K133A and E142O were 88.45, 84.70, and 83.60 kcalÆmol )1 , respectively. The melting point and DSC proﬁle of E142O (52.8 °C) was alm...

Ngày tải lên : 20/02/2014, 01:20

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Báo cáo khoa học: Local stability identiﬁcation and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... difference of DH cal of mutant from wild-type (%), heat capacity (DC p ) and difference of DC p of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants ... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G, E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 repr...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx

... Ulrih 1 and Tea Lanis ˇ nik Riz ˇ ner 2 1 Department of Food Science and Technology, University of Ljubljana, Slovenia 2 Institute of Biochemistry, University of Ljubljana, Slovenia Proteins of the ... studies of substrate speciﬁcity and expression analysis. Mol Cell Endocrinol 171, 193–198. 6 Andersson A, Jordan D, Schneider G & Lundqvist Y (1996) Crystal structure of...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: Thermodynamic stability of porcine b-lactoglobulin A structural relevance docx

... character of thermal denaturation of this protein at acidic and basic pH. The dependence of the conformational stability of porcine BLG on pH is analysed and discussed in comparison with that of bovine ... Thermograms of native (1) and renatured (2) porcine BLG at pH 9.0 (A) and pH 3.0 (B). Fig. 5. Thermograms of porcine (1) and bovine (2) BLG at pH 9.0 (A) and pH...

Ngày tải lên : 23/03/2014, 21:21

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Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx

... demonstrated the involvement of one of these clusters in the stability of the quaternary structure. The present study focuses on the role of the same cluster in the stability of the cofactor binding site. ... the inhibitory and denaturing effects of urea on the catalytic activity of the enzyme. The enzyme in the presence of urea is assumed to exist as a mixture of free...

Ngày tải lên : 30/03/2014, 02:20

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Báo cáo khoa học: Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content potx

... thermal stability of the homologous FUs of b-hemocyanin of H. pomatia and considered the role of the degree of glycosylation and the secondary structure variability on the observed differences in stability. Results The ... as a function of temperature. At 25 °C, the amide I¢ region of all b-HpH FUs consists of a broad band. Deconvolution and ﬁtting of the spectru...

Ngày tải lên : 30/03/2014, 04:20

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