Báo cáo khoa học: Hyperthermal stability of neuroglobin and cytoglobin doc

... T m . The enthalpy of denaturation DH m of Ngb and horse heart Mb was 72 and 110 kcalÆmol )1 , respectively. Cytoglobin and the globin of Drosophila have lower values of DH m , 60 and 53 kcalÆmol )1 , respectively. ... Ngb had negative bands at 208 and 222 nm (Fig. 1), as expected for a high percentage of alpha helix. Analysis of the secondary structure of Ngb gave 7...

Ngày tải lên: 30/03/2014, 16:20

... Alessandra Corazza 1 , Paolo Viglino 1 , Luc Moens 3 , Gennaro Esposito 1 and Angelo Fontana 2 1 Department of Biomedical Sciences and Technologies and MATI Centre of Excellence, University of ... structural properties of helices F in the two proteins, we analyzed pep- tides encompassing helix F of neuroglobin and myoglobin in the wild-type and mutant forms. NMR and CD...

Ngày tải lên: 30/03/2014, 01:20

... USA) and Anna Tramontano (University of Rome, Italy) with the invaluable help of Andriy Kryshtafovych (Lawrence Livermore National Laboratory) and Volker Eyrich (Columbia University). The goals of ... REVIEW ARTICLE Ten years of predictions … and counting Domenico Cozzetto 1 , Adele Di Matteo 1 and Anna Tramontano 2 1 Department of Biochemical Sciences, University of Rome...

Ngày tải lên: 07/03/2014, 16:20

... amino acid sequences of Mb and Ngb and the comparison of A B C D Fig. 2. CD characterization of Ngb and Mb at neutral and acidic pH. (A) Far-UV CD spectra of human holoNgb and apoNgb dissolved ... University of Padua, Italy 2 Department of Biochemistry, University of Antwerp, Belgium 3 Department of Zoophysiology, University of Aarhus, Denmark Introduction Neurogl...

Ngày tải lên: 07/03/2014, 02:20

... melting curves of wild-type and mutants of SNase. DSC curves of five proteins [wild-type (WT), W140A, E140O, W140O and K133A]. The curves of W140A and W140O are nearly linear in terms of intensity. ... 3961 of the wild-type protein and K133A and E142O were 88.45, 84.70, and 83.60 kcalÆmol )1 , respectively. The melting point and DSC profile of E142O (52.8 °C) was alm...

Ngày tải lên: 20/02/2014, 01:20

... difference of DH cal of mutant from wild-type (%), heat capacity (DC p ) and difference of DC p of mutant from wild-type (%) were determined and the results for the wild-type protein and 20 mutants ... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G, E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 repr...

Ngày tải lên: 07/03/2014, 21:20

... Ulrih 1 and Tea Lanis ˇ nik Riz ˇ ner 2 1 Department of Food Science and Technology, University of Ljubljana, Slovenia 2 Institute of Biochemistry, University of Ljubljana, Slovenia Proteins of the ... studies of substrate specificity and expression analysis. Mol Cell Endocrinol 171, 193–198. 6 Andersson A, Jordan D, Schneider G & Lundqvist Y (1996) Crystal structure of...

Ngày tải lên: 16/03/2014, 13:20

... character of thermal denaturation of this protein at acidic and basic pH. The dependence of the conformational stability of porcine BLG on pH is analysed and discussed in comparison with that of bovine ... Thermograms of native (1) and renatured (2) porcine BLG at pH 9.0 (A) and pH 3.0 (B). Fig. 5. Thermograms of porcine (1) and bovine (2) BLG at pH 9.0 (A) and pH...

Ngày tải lên: 23/03/2014, 21:21

... demonstrated the involvement of one of these clusters in the stability of the quaternary structure. The present study focuses on the role of the same cluster in the stability of the cofactor binding site. ... the inhibitory and denaturing effects of urea on the catalytic activity of the enzyme. The enzyme in the presence of urea is assumed to exist as a mixture of free...

Ngày tải lên: 30/03/2014, 02:20

... ther- mal stability of the homologous FUs of b-hemocyanin of H. pomatia and considered the role of the degree of glycosylation and the secondary structure variability on the observed differences in stability. Results The ... as a function of temperature. At 25 °C, the amide I¢ region of all b-HpH FUs consists of a broad band. Deconvolution and fitting of the spectru...

Ngày tải lên: 30/03/2014, 04:20