Báo cáo khoa học: Synthesis of b-mannosides using the transglycosylation activity of endo-b-mannosidase from Lilium longiflorum pptx

... in the absence of a Du,as shown in Fig. 3 C. In the presence of a Du, the difference between the two strains was twofold over the entire DpH range tested. In the absence of a Du, the ATP synthesis ... over the mutant, indicating the ratios of the ATP synthesis rates, are plotted for the exp eriments in the absence (dashed line) or in the presence (continu...

Ngày tải lên: 21/02/2014, 03:20

... 1) between stefin A and the different enzymes is the average distance between the centre of mass of CA atoms of the stefin molecule and the centre of mass of the CA atoms of the reactive site cysteine ... direc- tions. The side chain of H110 points away from the active site cleft to the back of the molecule, whereas the side chain of H111 points upwar...

Ngày tải lên: 18/02/2014, 04:20

... Down- regulation of the alpha-Gal epitope expression in N-gly- cans of swine endothelial cells by transfection with the N-acetylglucosaminyltransferase III gene. Modulation of the biosynthesis of terminal ... N-glycans located on either the b-propeller of a 5 [55] or the I-like domain of b 1 or b 3 [68] contribute to the regulation of integrin function. Therefore, we...

Ngày tải lên: 18/02/2014, 17:20

... change the preference of site utilization; and (c) promotion of the dissociation of Chordin cysteine-rich (CR)-containing fragments from the ligand. They suggest that the first of these three is the ... Smad4). The complexes move into the nucleus and act as regulators of transcription. The activity of BMP is regulated by the binding of extracellular inhibito...

Ngày tải lên: 19/02/2014, 00:20

... torque of 112 pNÆnm, the arrows show the beginning (t ¼ 1ns)andtheend(t¼ 23 ns) of the forced rotation. With 56 pNÆnm torque, the relaxation of the system was calculated during the last 8 ns of the ... because the samples were diluted during the activity a ssay and a dded ATP displaced the residual amounts of AMP-PNP and ADP from the catalytic sites of t...

Ngày tải lên: 19/02/2014, 16:20

... diphosphoribose. The development of novel cADPR analogues revealed new insights into the structure activity relationship. Substitution of either the northern ribose or both the northern and southern ribose resulted ... between the base adenine (or hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands. These ether strands mimic the dis...

Ngày tải lên: 20/02/2014, 01:20

... if the actions of the CHH neuropeptides on repression of ecdysteroid synthesis by the Y-organ (YO) are considered. The most widely accepted paradigm of moult control in crustaceans concerns the ... pooled extracts of incubation media (from these experiments) were analyzed by HPLC-RIA, a reduction in the ability of MIH (5 n M ) to repress synthesis of both ecdysone...

Ngày tải lên: 21/02/2014, 00:20

... number of two-state transitions. In these fittings the heat capacity change, DC p ,forthe unfolding of each domain was fixed by using the values obtained from the analysis of the C p curves of the corresponding ... character- ization of the thermally induced aggregation of SK using a variety of techniques. The results provide us with some of the properties...

Ngày tải lên: 21/02/2014, 03:20

... representative of the totality of the single-stranded overhang, and which may be important for these ligands being able to differentiate telomeric quadruplexes from others in the genome. It has ... ultimately apoptosis. The catalytic subunit of telo- merase (hTERT in humans) has reverse transcriptase enzymatic activity and synthesizes TTAGGG repeats on to the end of the...

Ngày tải lên: 06/03/2014, 09:22

... accele rated in the absence of TF probably by the disclosure of a more direct cotranslational targeting pathway to the Sec translocon [3]. Perhaps, the proofreading activity of the Sec t ranslocase ... indicative of cross- linking to the released full-length form of SufIHA. Notably, an identical  170 kDa crosslinked complex was observed upon completion of synthesis...

Ngày tải lên: 07/03/2014, 16:20