Báo cáo khoa học: Mitochondrial affinity for ADP is twofold lower in creatine kinase knock-out muscles Possible role in rescuing cellular energy homeostasis pptx

... (2005) 956–965 ª 2005 FEBS 965 Mitochondrial affinity for ADP is twofold lower in creatine kinase knock-out muscles Possible role in rescuing cellular energy homeostasis Frank ter Veld 1 , Jeroen ... (1992) Intracellular compartmenta- tion, structure and function of creatine kinase iso- enzymes in tissues with high and fluctuating energy demands: th...

Ngày tải lên: 30/03/2014, 15:20

... cells). In the presence of 20 mm EDTA, the binding of Fc-fusion proteins to Lec8 cells was abolished. We then performed inhibition tests using a GlcNAc- binding lectin from Psathyrella velutina (PVL). ... were pre-incubated in the precomplex solutions at room temperature for 30 min. Binding of DC-SIGN-related lectin–Fc chimeras to cells Cells (1 · 10 6 ) in TSA buffer [NaCl ⁄ Tris con...

Ngày tải lên: 15/03/2014, 23:20

... Seurin D, Segovia-Quinson B, Hardouin S & Binoux M (1986) Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding ... residing in the heart of the IGF-II binding domain in repeat 11 disrupts IGF-II binding to the receptor [22,31–33]. The ligand blotting data shown in Fig. 1C confirm that the wild- type min...

Ngày tải lên: 18/02/2014, 08:20

... two per se interesting and novel facts: (a) that human stefin B (cystatin B) is a high affinity copper binding protein, and (b) that amyloid fibril formation of this protein is diminished in presence of ... binding in many proteins they probably form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another histidine is presen...

Ngày tải lên: 19/02/2014, 06:20

... ecdysone agonists by measuring the induction of chitin synthesis in the cultured integument of C. suppressalis larvae; the 50% effective concentration (EC 50 ) for the induction of the chitin synthesis in ... EcR–USP complex expressed in vitro in this study is a functional and useful material for using in binding assays of ecdysone agonists. In a previous study, we evaluated...

Ngày tải lên: 23/03/2014, 21:20

... of HSEs in HS response in rice. The formation of the trimeric form of Hsfs is con- sidered important for attaining their high affinity bind- ing to HSEs [19]. Using in vitro crosslinking and yeast two-hybrid ... provided support in indicating that Hsfs show protein–protein interactions. This tech- nique has also been used for visualization of the subcel- lular locations of the...

Ngày tải lên: 05/03/2014, 23:20

... Ssp1 kinase is involved in the G2 ⁄ M transition [39], so that glucose metabolism may be related to cell cycle regulation. In humans, calcium– calmodulin-dependent protein kinase kinase has a role in ... has a role in utilizing glucose through interaction with AMP- dependent protein kinase [40], which is implicated in diabetes [41]. In S. pombe, Ssp2 is the AMP-dep...

Ngày tải lên: 14/03/2014, 23:20

... [poly(viny- lidene difluoride)] membrane (Millipore Corp, Bedford, MA, USA); nonspecific binding sites w ere blocked by incubation in 5% (w/v) n onfat powdered milk in buffer containing 50 m M Tris/HCl, ... the genes a nd proteins req uired for the P450scc system are expressed concomitantly in the skin and skin cells. Moreover, using an array of methods including chemical synthesis with...

Ngày tải lên: 23/03/2014, 13:20

... 17b-HSD-11 gene in the intestine is clearly important and we are trying to solve this problem in our laboratory hoping to uncover a new role of PPARa in this organ. Possible roles of 17b-HSD-11 in the intestine Mouse ... examined the PPARa agonist-induced proteins in the intestine, another important organ for lipid metabolism e xpressing a fairly large amount of PPARa in m...

Ngày tải lên: 16/03/2014, 18:20

... analysis. R. solani resistance analysis on transgenic plants Cultures of R. solani AG-4 were incubated in the dark at 30 °C for 48 h on PDA plates, and maintained at 23 °C for 2 weeks before use in ... Thaumatin-like inhibitors [12]. Kunitz PIs are single-chain polypeptides of around 20 kDa with low cysteine content, generally with four cysteine residues arranged into two intra-chain...

Ngày tải lên: 23/03/2014, 03:20