Báo cáo khoa học: Characterization of the secreted chorismate mutase from the pathogen Mycobacterium tuberculosis potx

... [24,25]. Adjustments of the cell fractionation proto- col involved reducing the starting material and the volume of the sucrose layers but maintaining the relative ratios of the sucrose layers. ... 2006 The Authors Journal compilation ª 2006 FEBS 3293 On the basis of conservation of initial steps in the N-glycosylation pathway and divergence of this path- way fol...

Ngày tải lên: 16/03/2014, 13:20

... b-amylase (200 kDa). The elution volume (V e ) of the marker proteins and of the HP0050 protein was determined. The molecular mass of the HP0050 protein was calculated from a plot of V e ⁄ V o versus ... hydro- dynamic radius of the average scattering molecule. The theoretical hydrodynamic radius (R theo h) for the protein under investigation is calculated from...

Ngày tải lên: 22/03/2014, 21:20

... intensity, e.g. for the E81Q mutation, the dependence of the m(C = O) signal for up to 50% of its intensity on the orientation of the methoxy side chains in relation to the position of the quinone ring ... difference spectra of the site-directed mutations in the Q o bind- ing site of the bc 1 complex from P. denitrificans,a small bacterial version of the m...

Ngày tải lên: 23/03/2014, 10:20

... AF481963. The open reading frame encodes a 130-amino acid polypeptide. The comparison of the amino acid sequence deduced from the cDNA sequence with that of the N-terminal sequence of the natural ... equivalent (Qeq), the average amount of pheromone found in the gland of mated queen [50]. The synthetic pheromone blend was dissolved in ethanol to a final concentrat...

Ngày tải lên: 21/02/2014, 03:20

... membranes. The amounts of protein loaded onto gels were as follows: for the PSD- 95 blot, 70 lg of TM, 96 lg of LM, 89 lg of MM, and 93 lgof HM; for the calnexin blot, 70 lg of TM, 96 lg of LM, 89 lg of ... distinct from POP, on the basis of the difference between their molecular masses (87 kDa versus 65 kDa) obtained in gel filtration experiments [9,10]. The theo...

Ngày tải lên: 07/03/2014, 06:20

... in the supernatant, the residue being basically devoid of sugars (Fig. 1A). In agreement with solubilization of the sugars being caused by release from mucins, the kinetics of mass loss from the ... observed that the alkaline hydrolysis used to free the LL glycans from their putative mucin cores solubilized the mater- ial with the exception of a solid that diss...

Ngày tải lên: 07/03/2014, 12:20

... I 0 Þdv ð1Þ where n res is the number of residues of the protein (assumedtobe140),A 90° is the absorption with the 90 ° polarizer, and S are order parameters calculated from the ratio of the parallel and ... hydrated, and then on deuterated films, with or without a polariser set at either 0 ° or 90 ° (with respect to the plane of reflections). In the case of lipid-bo...

Ngày tải lên: 23/03/2014, 21:20

... be rationalized by the fact that the normal site of action for a CM in E. coli is the cytoplasm, where the enzymes of the shikimate pathway are located. The data from the complementation assays, the cellular ... tuberculosis FEBS Journal 272 (2005) 375–389 ª 2004 FEBS 389 Characterization of the secreted chorismate mutase from the pathogen Mycobacter...

Ngày tải lên: 30/03/2014, 15:20

... characterized by the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produce adult tissues (i.e. the development of the gills and the production of ... was based on the conserved amino acid sequences of the catalytic domain of members of the GH18 family resulted in the amplification of an expected 14...

Ngày tải lên: 19/02/2014, 00:20

... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers in parentheses below the diagram show the position of the corresponding ... of pGL3–basic vector and was half that of pGL3–promoter vector. These findings demonstrate the promoter activity of the 5¢-flanking region of exon 1 in...

Ngày tải lên: 19/02/2014, 06:20