Báo cáo khoa học: Stability of the major allergen Brazil nut 2S albumin (Ber e 1) to physiologically relevant in vitro gastrointestinal digestion doc

... physiologically relevant in vitro model sys- tem either before or after heating (100 °C for 20 min). Whilst the albumin was cleaved into peptides, these were held together in a much larger struc- ture even ... DESCRCEGLRMM(20– 31) of the large subunit (Table 1). Pepsinolysis removed either the N-terminal Asp or the C-terminal Met, giving rise to peptides ESCRCEGLRM(2...

Ngày tải lên: 30/03/2014, 15:20

... disaster in southeast Asia severely injured tourists were repatriated via airlift to Germany. One cohort was triaged to the Cologne-Merheim Medical Center (Germany) for further medical care. We report on ... vacuum-assisted closure in the interim between initial wound surgery and secondary closure. All patients received empiric anti-infective therapy using quinolones and clindamyc...

Ngày tải lên: 25/10/2012, 10:41

... post-tran- scriptional effects, and most likely changes in protein secretion, the mRNA levels of each lysozyme variant relative to the endogenous genetic reference b-actin, were determined by reverse transcriptase ... correlation has been identified between the level of secreted protein and the thermo- stability of the native state of the protein (Fig. 2A). This corre...

Ngày tải lên: 19/02/2014, 08:20

... glycine is nonconservative, we introduced alanine at these two positions, with the aim of determining whether any of the observed effects were a result of changes in the size and charge of the ... structure, observed in the CD spectrum, affects this part of the protein, or whether there is transient unfolding of the end of this helix. There are examples of he...

Ngày tải lên: 16/03/2014, 06:20

... obtained by MALDI MS (28 664 Da) by the number of amino-acid residues of the mature protein sequence (225 residues). Thermal denaturation experiments were carried out by increasing the temperature ... behaviour. Discussion The near-UV CD spectrum of PSP-I ⁄ PSP-II reflects the specific environment of chiral aromatic side chains in the tertiary structure of the folded p...

Ngày tải lên: 23/03/2014, 15:21

... collagen-like peptides increases the stability of the triple-helical structure by a small margin. Insertion of 3(S)Hyp in the context of the nine tripeptide units increases the T m of the peptides ... indicates the importance of 4(R)Hyp in the nucleation process. Future studies will be performed to study the mechanism of refolding of these model peptide...

Ngày tải lên: 30/03/2014, 02:20

... name of the corresponding mutants. Error bars represent errors from the fit to determine k bim . The solid line represents a linear fit to the data points, dashed lines mark the 95% confidence intervals. ... between log k U0 and the Gibbs energy of unfolding at 0 [urea] (DG U0 ). In the mutant series, the overall height of the energy barrier for the rate-determini...

Ngày tải lên: 30/03/2014, 20:20

... partially to these vari- ations. Results Steady-state levels of cyclin mRNA in the cell cycle In a first series of experiments we determined whether mRNA steady-state levels of cyclins and several cell cycle-related ... cells, essential regulatory steps are likely to be conserved in evolution and thus amenable to genetic analysis in the mouse. We therefore examined mRNA...

Ngày tải lên: 30/03/2014, 20:20

... the single-molecule level. The analyses of individ- ual SERCA molecules in the presence of both ATP and free Ca 2+ revealed up–down structural changes corresponding to the Albers–Post scheme. This fluctuation ... of the projection of the embed- ded molecule above the flat membrane surface, the average height was 5.4 ± 0.8 nm, which is in good agreement with the h...

Ngày tải lên: 14/02/2014, 14:20

... Dozens of bacte- rial factors have been identified to in uence the patho- genesis of H. pylori. There are two classical secreted virulence factors present in H. pylori: the vacuolating cytotoxin ... triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular milieu. (B) Model-1 for the...

Ngày tải lên: 14/02/2014, 19:20