Báo cáo khoa học: Regulation of phospholipid biosynthesis by phosphatidylinositol transfer protein Sec14p and its homologues pot

... Regulation of phospholipid biosynthesis by phosphatidylinositol transfer protein Sec14p and its homologues A critical role for phosphatidic acid Roman Holic ˇ , Milos ˇ Za ´ gors ˇ ek and ... transcriptional regulation of phos- pholipid biosynthesis. The results show that (a) none of the Sec14 homologues w as able to substitute for Sec14p in its regulato...

Ngày tải lên: 30/03/2014, 15:20

... Activation of p38 mitogen-activated protein kinase and c-Jun NH(2)-terminal kinase by double- stranded RNA and encephalomyocarditis virus: involve- ment of RNase L, protein kinase R, and alternative pathways. ... Canadian Institutes of Health Research and CANVAC, the Canadian Network for Vaccines and Immunotherapeutics. N.G. was supported by a post- doctoral FRSQ fello...

Ngày tải lên: 07/03/2014, 21:20

... covalent modifications, protein protein interactions with negative regulatory proteins and proteolytic processing by pro- teases. Cleavage of STAT proteins by proteases results in the generation of C-terminally ... activity of STAT proteins (Fig. 2). These include the activities of phosphatases, suppressors of cytokine signalling (SOCS), interaction of inhibitory proteins s...

Ngày tải lên: 30/03/2014, 15:20

... recruitment by DNA-bound SRF of an, as yet, unidentified accessory factor [54]. The regulation of c-jun is complex and may involve an increase in the levels of c-jun protein and/ or phosphorylation of specific ... Expression of the proteins encoded by Ca-RhoA, Ca-Cdc42 and Ca-Rac constructs as shown by Western blot analysis of cells transfected with the corresponding...

Ngày tải lên: 08/03/2014, 08:20

... digested with SalI and NotI and then inserted into the SalIandNotIsitesof pCI-neo-Flag or pCI-neo-c-6Myc. Immunoblotting Proteins were separated by SDS/PAGE on a 10% gel and transferred to a nitrocellulose ... repre- sentation of the structures of the As-rel genome and mRNAs of As-rel1 and As-rel2. (B) Genome sequence of splicing sites. Note that As-rel1 mRNA is generated...

Ngày tải lên: 30/03/2014, 20:20

... sterol biosynthesis may increase during formation of a mating projection [28]. Furthermore, homologues of oxysterol- binding proteins, a family of proteins that regulate the synthesis and transport ... establishment and maintenance of cell polarity (e.g. by counteracting lateral diffusion of polarized proteins within the membrane) [58,62]. Alternatively, sterols may be imp...

Ngày tải lên: 18/02/2014, 13:20

... immunization and bleeding of rabbits; to Silvia Zezlina for the affin- ity purification of antibody A from rabbit sera; to Dr Paolo Ermacora and Prof Giorgio Honsell (University of Udine) and Mr Fulvio ... acid free BSA and 20 mm Tris ⁄ HCl pH 7.5 at a final protein concentration of % 1mgÆmL )1 . Marker enzyme assays The level of purification of tonoplast and plasma mem- b...

Ngày tải lên: 20/02/2014, 01:20

... [ 32 P]ATP[aP]) and the photoregulatory efficiency of transcription (a) was Fig. 1. Sequences of photoresponsive T7 promoters and the structures of Azo and its derivatives (M-azo and DM-azo) used ... instead of Azo (Fig. 1). Clear-cut on–off photoregulation of DNA transcription was obtained because of the efficient suppression of T7 RNA polymerase (RNAP) binding to the D...

Ngày tải lên: 15/03/2014, 10:20

... reactions of rGlmM were performed in the pres- ence of 5 mM CoCl 2 . Proteins were separated by SDS ⁄ PAGE, and radioactive bands revealed by autoradiography. Positions and molecular mass (kDa) of protein ... phos- phorylation of S1 protein and a-subunit of RNAP are important for their interaction. The interaction of RNAP and S1 protein has already been described...

Ngày tải lên: 16/03/2014, 18:20

... Academy of Sciences, Warsaw, Poland 3 Department of Physiological Chemistry and Center for Biomedical Genetics, University Medical Center Utrecht, the Netherlands Phosphorylation of proteins by protein ... Burgering 3 and Lech Trzeciak 1 1 Department of Molecular Biology, International Institute of Molecular and Cell Biology in Warsaw, Poland 2 Laboratory of Molecular Me...

Ngày tải lên: 23/03/2014, 11:20