Báo cáo khoa học: Cloning of two melanocortin (MC) receptors in spiny dogfish MC3 receptor in cartilaginous fish shows high affinity to ACTH-derived peptides while it has lower preference toc-MSH ppt

... 4331 Cloning of two melanocortin (MC) receptors in spiny dogfish MC3 receptor in cartilaginous fish shows high affinity to ACTH-derived peptides while it has lower preference to c-MSH Janis Klovins 1,2 , ... any specific binding with this receptor. Membranes containing SacMC3R were used to test the binding properties of the endogenous melanoc...

Ngày tải lên: 30/03/2014, 15:20

... made up of two f unctional domains, one corresponding to the cofactor-binding domain, or Rossman fold (residues 1–147 and 313–334 in spinach GAPDH (accession code in PDB:1JN0), the other being the catalytic ... directly involved in the interaction with the small protein. It is likely that the introduction of the negative charge destabilizes the S loop, thus indicating that thi...

Ngày tải lên: 19/02/2014, 16:20

... causes the partial inhibition of PSII, and leads to a reduction of photosynthetic O 2 evolution. The partial inhibition of PSII is sufficient to create anaerobic culture conditions and induce H 2 -production ... levels of HydA1 increased up to about 48 h, corresponding to the time when H 2 -production rates are high (Fig. 3, top). In contrast to HydA1, the levels of the...

Ngày tải lên: 20/02/2014, 11:20

... a tightening up of the site in the latter together with a movement of the His106 side chain away from the fla- vin and towards the substrate [its ring nitrogen atom that participates in hydrogen bonding ... operates in con- cert with N(5) of the cofactor to bring about the abstraction of the C(6proR) hydrogen of the substrate [13]. In addition to these invariant amino aci...

Ngày tải lên: 07/03/2014, 05:20

... events involved in converting phytoene to lycopene. CrtIb, on the other hand, is incapable of dehydrogenating phytoene and lacks cis -to- trans isomerase activity. However, the presence of both ... r 70 consensus binding site, TTGACA, in position ) 35 and TATAAT in position ) 10 [41], and was cloned in vectors digested with HindIII and PstI. The sequence between the ) 35 and )...

Ngày tải lên: 16/03/2014, 05:20

... D11 desaturation of myristoyl-CoA in insects [21]. It is of special interest that the two isomeric products of this desaturation in insects are precursors for two different pheromones with different ... different ratio of oleate to linoleate indicate that this ratio determines the ratio of utilization of the two substrates by the acetylenase. The ratio of o leate...

Ngày tải lên: 16/03/2014, 18:20

... protein-coupled receptors [e.g. serotonin (5-hy- droxytryptamine 2c) receptor, non-N-methyl-d-aspar- tate glutamate receptor channels in mammals, and squid K + channels in invertebrates] [40]. Interestingly, if ... is critical in rat for CNR1 receptor activation of K + and Ca 2+ channels [30]; (e) a leucine and alanine pair in the C-terminus of the third cytoplasmic loop im...

Ngày tải lên: 30/03/2014, 08:20

... at position 268 with a glycine determining A activity whereas an alanine determines B activity. A glycine is present at the homologous position in the rat sequence (position 263), suggesting a ... herein has a glycine at the position equivalent to human 268 in accordance with its A enzymatic activity. Similar to the pig enzyme [26], but at variance with the human A enzymes, it ha...

Ngày tải lên: 31/03/2014, 09:20

... topology for injection of CagA is not yet resolved. It is also unclear why the effector protein CagA itself can bind to the extracellular domain of inte- grin b 1 because such high binding affinity would ... basal membrane proteins to migrate apically. Transcytosis of integrins would therefore enable H. pylori with the intriguing possibility of targeting the integrin b 1 recep...

Ngày tải lên: 14/02/2014, 19:20

... C-terminal halves of ubiquitin (N-Ubi and C-Ubi) alone do not assemble. If a protein ‘X’, fused to N-Ubi, binds to the PAKs Ste20p or Cla4p, linked to C-Ubi, both halves of ubiquitin are brought into ... important in the association of proteins involved in establishing cell polarity with the plasma membrane, which occurs independently of endocytosis. It has been suggeste...

Ngày tải lên: 18/02/2014, 13:20