Báo cáo khoa học: Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells docx
Báo cáo khoa học: Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells docx
... Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells Jorge Lora, Francisco J. Alonso, Juan A. Segura, Carolina ... normal cells. In the present work, Ehrlich ascites tumour cells, and their derivative, 0.28AS-2 cells, expressing antisense glutaminase mRNA, were assayed f...
Ngày tải lên: 30/03/2014, 15:20
Báo cáo khoa học: p53-induced inhibition of protein synthesis is independent of apoptosis pdf
... counting. Preparation of cell extracts and analysis by immunoblotting Cytoplasmic extracts were prepared for immunoblotting by washing the cells in NaCl/P i and lyzing them in a buffer containing ... mutant in MEL cells (or of the equivalent Val138 mutant in human cells) can be achieved by reducing the incubation tempera- ture from 38 to 32 °C and results in inhibition...
Ngày tải lên: 23/03/2014, 18:20
Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... is noninfective and proliferates extracellularly in the insect gut where it differentiates into metacyclic trypomastigotes, which can then infect the mammalian host cells and replicate intracellularly ... con- taining DNA spots (5 lg each) corresponding to inter- nal fragments of ADC or cruzipain genes. The latter (used as a control) is a housekeeping gene that encodes the main cystei...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf
... constructs, spectinomycin (100 lgÆmL )1 )wasused. Proteins, GAGs, radiolabelling and binding assay Recombinant protein H, M1 protein and the A-S and S-C3 fragments of M1 protein were prepared ... region, IgGFc-binding to the S-domain, and albumin-binding to the C-repeats [31]. Recombinantly expressed fragments rA–S and rS–C3 are indicated. (D) The M1 protein and the A–S and S–C3...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf
... proteinase with a limited distribu- tion in certain cell types, including gastric epithelial cells [4], but is mainly present in cells of the immune Keywords antigen-presenting cells; cathepsin ... brain [23]. CatE and CatD both play an important role in the MHC class II pathway. CatD is reported to be involved in processing MHC II-associated invariant chain [24] in antigen p...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
... (Ala253 and Val254 in LDH are replaced by Val253 and Ile254 in LDHB). The histidine and argi- nine residues directly involved in catalysis are identical in the two enzymes, and NADH binding is ... present in the parental strain and in the mutant FI9078 were due to homofunctional enzymes displaying clear differences in kinetic and reg- ulatory parameters. Therefore, w...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt
... dissected and preincubated in Ringer’s medium for 15 min at 22 °C. Radioactive labeling of newly synthesized proteins was per- formed by incubating the NILs in Ringer’s medium con- taining 5 mCiÆmL )1 [ 35 S]-Met ... been studied only in cultured (transfected) tumour cell lines and not in primary cells. Here, we investigated the intracellular fate of PrP C in vivo by using th...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: "An Architecture for Dialogue Management, Context Tracking, and Pragmatic Adaptation in Spoken Dialogue Systems" pot
... chitecture is distinguished from these in its treatment of discourse-level processing. Most architectures, including ours, contain mod- ules for speech recognition and natural language interpretation ... Tracking The Context Tracker maintains a record of the discourse context which it and other components can consult in order to (a) resolve dependent forms that occur in inp...
Ngày tải lên: 17/03/2014, 07:20
Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... seedlings [27]. Macropinocytosis dependent transduction of fluores- cent proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and ... combined use of cell per- meabilizing agent and cell-penetrating peptides results in the delivery of such large sized cargoes in plant cells involving endocytic ⁄ macropinocytic pathw...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... nitrate in air. (F) Ornithine, citrulline and arginine contents in leaves of Arabidopsis plants cultured with 2 m M ammonium in air. (G) Ornithine, citrulline and arginine contents under high-CO 2 conditions. ... overall arginine levels. The levels of ornithine, citrulline and arginine remained low, at between 0.01 and 0.04 lmolÆg )1 fresh weight (Fig. 3E). However, arginine acc...
Ngày tải lên: 30/03/2014, 01:20