Báo cáo khoa học: Crystal structures of bovine odorant-binding protein in complex with odorant molecules doc

... J. Biochem. 271) Ó FEBS 2004 Crystal structures of bovine odorant- binding protein in complex with odorant molecules Florence Vincent 1 , Roberto Ramoni 2 , Silvia Spinelli 1 , Stefano Grolli 2 , ... changes involving protein loops or backbone. T hus, the role of OBP in signal triggering remains unresolved. 3 Keywords: crystal str ucture; domain swapping; odora...

Ngày tải lên: 30/03/2014, 15:20

... inserted into the ACT2 domain; and (c) two ACT domains, each from a different chain, interact to form an effec- tor-binding unit. The effector-binding unit of the ACT domain in the b-subunit of CgAK ... effector-binding unit formed between two chains in TtAKb in a manner almost identical to that in CgAKb, suggesting that Thr binding plays a role in stabilizing the dimeric form...

Ngày tải lên: 18/02/2014, 08:20

... steric in uence on cofactor binding. The adenine stacks against the Arg34 guanidinium group. This arginine, essential for NADP + binding [19], becomes well ordered in the ternary complex with NADP + . ... by incubating protein with ligands in a 1 : 5 ratio. Complex III was prepared by mixing the pro- tein, NADP + and inhibitor in a ratio of 1 : 5 : 10. Crystals were grown...

Ngày tải lên: 19/02/2014, 05:20

... Effects of the in- vitro fer- mentation of oligofructose and inulin by bacteria grow- ing in the human large-intestine. J Appl Bacteriol 75, 373–380. 12 Fooks LJ & Gibson GR (2002) In vitro investigations of ... the protein sequence of the target protein. This model was used to solve the crystal structure of the native b-fructofuranosidase with the phaser [53] progr...

Ngày tải lên: 06/03/2014, 00:21

... fea- tures with other viral glycoproteins responsible for membrane fusion, including the hemagglutinin (HA) protein in influenza virus, gp160 of HIV-1, GP of Ebola virus and the spike protein of severe ... consist of two surface glycoproteins on the viral surface, termed the fusion (F) protein and glycoprotein (G protein, also called attachment protein) [7,8,12,13]. These t...

Ngày tải lên: 08/03/2014, 08:20

... isopeptide bond with its C-terminal glycine. There are at least four SUMO proteins in humans, wh ich are involved in protein trafficking and tar- geting. A truncated human SUMO-2 protein that contains residues ... collection. The S UMO-2 protein was purified using a column packed with Ni–NTA HisBindÒ resin ( Novagen) in two steps. In the first purification, major protein was el...

Ngày tải lên: 16/03/2014, 18:20

... protease crystal structures with indinavir (Eur. J. Biochem. 271) 1519 inhibitor. Thus, subtle conformational changes of residues interacting with the inhibitor play a role in the kinetics. The ... the two Cc atoms of Val82 formed van der Waals contacts with indinavir, of 3.8 A ˚ .In PR V82A , the change in the position of the main chain atoms placed the Cb atom of A...

Ngày tải lên: 23/03/2014, 12:20

... diverse family of proteins involved in the metabolism of amino acids, amino sugars and amino group containing lipids. A majority of them are key enzymes in the metabolism of amino acids. The ... Non- specifically bound proteins were removed by a wash with buffer B containing 20 mm imidazole. In the last step, the protein was eluted using buffer B containing 200 mm imid- azole...

Ngày tải lên: 28/03/2014, 22:20

... described in (A) and (B). (E) Alignment of the primary structures of hinge-1 and hinge-2 of TvuCMBP and related solute-binding proteins. PfuMBP, P. furiosus maltodextrin-binding protein (amino acid ... modes of ligand binding in maltose-binding protein of Escherichia coli. Functional significance in active transport. J Biol Chem 272, 17615–17622. Open ⁄ closed cyclo ⁄ mal...

Ngày tải lên: 29/03/2014, 23:20

... containing 10 mgÆmL )1 of protein, 30% (w ⁄ v) PEG 1000, and 0.1 m of Tris ⁄ HCl at pH 8.5. Refinement of preliminary crystalliza- tion conditions resulted in the growth of quality crystals in a ... determined by a BioRad protein assay kit using bovine serum albumin as a standard. The purified recombinant AeKAT was concentrated to 10 mgÆmL )1 protein in 5 mm phosphate buffe...

Ngày tải lên: 30/03/2014, 16:20