Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf

... cysteine and/or histidine residues, forming a zinc finger domain that is mostly involved in DNA binding but also in protein–protein interactions [17]. Originally identified as being present in different ... the coupling of zinc- binding structures (either in MT or in zinc finger proteins) by selenium compounds with the cellular r edox system GSH/GSSG, reducibl...

Ngày tải lên: 30/03/2014, 15:20

... indicate that the binding is dominated by hydrophobic interactions, which is in agreement with inclusion complex formation of c-CD. In addition, NMR measurements showed that in solution the spectra of GA ... thermodynamic parameters obtained are in the range of those of other interactions with c-CD [15]. The interaction of GA with c-CD is accompanied by favorable DS...

Ngày tải lên: 16/03/2014, 14:20

... that contains specific binding sites for the lyn kinase [31]. These findings provide a rationale for investigating whether caspase-mediated path- wayscaninteractwithPDE5inintactcells. In this article, ... plasmid construct following complex formation with LipofectAMINE TM 2000, accord- ing to the manufacturer’s instructions. The cDNA contain- ing media was then removed following incubation...

Ngày tải lên: 17/03/2014, 09:20

... HsRad51 to examine whether these loops are actually involved in DNA binding. Because aromatic residues are involved in the ssDNA binding by bacterial single stranded DNA- binding protein (SSB) and ... 3149 alanine. If the aromatic side chain is involved in the interaction with DNA, then its replacement with alanine, a short side chain amino acid residue, should affect th...

Ngày tải lên: 19/02/2014, 06:20

... polymerization of intact actin. (A) Effect of HSP25 and its 3D mutant on the kinetics of actin polymerization. Samples containing 1–4 l M of actin (10% of pyrene-labeled protein) were incubated for 5 min in ... the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the first series of exper...

Ngày tải lên: 20/02/2014, 23:20

... feedback-inhibited form of GS binds tightly to TnrA, preventing its binding to DNA, with the most effective feedback inhibitors of GS being glutamine and AMP [9]. Mutations in TnrA that result in constitutive ... coimmunoprecipitation (View interaction) l tnrA physically interacts with glnK by anti bait coimmunoprecipitation (View interaction) l tnrA binds to tnrA by cross-li...

Ngày tải lên: 06/03/2014, 00:21

... containing amino acids 98–110 of the flavivirus E protein) may serve as an internal fusion peptide, as it is directly involved in interactions with target membranes during the initial stages of membrane ... the interactions of an N-terminal and an internal peptide sequence of the E2 structural protein of HGV ⁄ GBV-C with model mem- branes, in order to understand the possibl...

Ngày tải lên: 07/03/2014, 17:20

... 17.6 Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of 5 mNÆm )1 . The proteins were injected into the ... [15]. In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers. Interaction of stefin B in the prefibrillar...

Ngày tải lên: 07/03/2014, 17:20

... ND49 with CP is because of deletion of the interacting domain. Discussion MPs are a diverse group of non-structural proteins of plant viruses that are involved in the spread of infection from ... major interacting domain within the N-terminal 49 amino acids of the SeMV MP. This is in contrast to observations on CPMV MP, in which the C-terminal domain was shown to be i...

Ngày tải lên: 15/03/2014, 00:20

... amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the protein was incubated with 1 ng of the indicated ... form with it the 100-kDa complex [21]. Figure 3B shows the binding of labelled oligomers to the nuclear-enriched fraction of proteins. The effi- ciency of labelling of the...

Ngày tải lên: 16/03/2014, 13:20