Báo cáo khoa học: Biochemical and enzymological aspects of the symbiosis between the deep-sea tubeworm Riftia pachyptila and its bacterial endosymbiont pptx

Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties pdf

... speciﬁc exhibition of a four-bandpattern was observed. The two lower bands represent the normal and mutated homoduplexes and, the upper bands correspond to the two types of the normal ⁄ mutated ... activity), further investigation of the contribu-tion of the mutation p.Gln204Lys was required. Given the extremely low residual activity of most of the mutant proteins, further kinetic studies of thosemutants ... a subtle change: the shortening of the bridge length by one methylenegroup. The abolition of the activity demonstrates the importance of an exact geometry in the interior of the enzyme.c.771...

Báo cáo khoa học: A modelling approach to quantify dynamic crosstalk between the pheromone and the starvation pathway in baker’s yeast pot

... Apart from the obvious nonlinear correlation between C and F (the one is the reciprocal of the other), the correla-tions differed considerably between activation measures,PREPs and FREPs, and crosstalk ... correla-tion between the integral and the maximum (mean rS¼0.9 ± 0.1) and a medium correlation between tM and s(rS¼ 0.3 ± 0.2 and rS¼ 0.7 ± 0.0 for PREPs and FREPs,respectively). The other ... correlation between C and Sefor the PREPs and between C and Sifor the FREPs (Fig. 5). Of course, all measures that are correlated with C are alsocorrelated with F.Considering the deﬁnitions of the...

Báo cáo khoa học: Vps4 regulates a subset of protein interactions at the multivesicular endosome doc

... components of the MVB sortingmachinery interact with Vps4p via the N-terminalMIT domainTo determine whether there is any correlation between the binding sites on Vps4p and the response of the interacting ... conserved between the yeast and humanVPS4 isoforms. In the Vps4p–IRA mutant, residues56–71 in the third helix of the MIT domain were dele-ted. These residues are also highly conserved. The YEE and ... decreased in the presence of ATP compared with binding in the absence of ATP. However, the binding of Did2p to Vps4p wasnot affected by the presence of ATP (Fig. 2).To determine whether the decreased...

Báo cáo khoa học: Hepatocyte-speciﬁc interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc

... 1AxoFenon2AxoF3AxoFenonPαBE/CSSPβBE/CSS1AxoFSS2AxoFSS1AxoFRat-1FTO-2BSS1AxoFFig. 1. Expression of CPS and its regulating transcription factors in Rat-1 and FTO-2B cells. (A) CPS and ... the lowerstrand. FoxA binding resulted in protection of the gua-nines at positions 347 and 351 on the upper strand, and protection at position 352 on the lower strand.These footprints in the ... factors [12]. One of the key determinants of the activation of genes that areunder the control of hormone response units is the order and time at which the transcription factors bindto their response...

Báo cáo khoa học: Biochemical and enzymological aspects of the symbiosis between the deep-sea tubeworm Riftia pachyptila and its bacterial endosymbiont pptx

... Minic and G. Herve´(Eur. J. Biochem. 271) Ó FEBS 2004REVIEW ARTICLE Biochemical and enzymological aspects of the symbiosis between the deep-sea tubeworm Riftia pachyptila and its bacterial endosymbiont Zoran ... speciﬁc and obligate nature o f t he symbiosis between vestimentiferans and their bacterial endosymbiont raises the question of the transmission of the bacteria fromone worm gen eration to the following ... products between the e nvironment and the animal (Fig. 2 ). The other tissues are within the Riftia tube. The vestimentum is a muscle that the animaluses to position itself in the tube. Within the...

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... kcat of 7.1 s)1 and Km of 0.182 mm toward NADP, kcat of 5.2 s)1 and Km of 2.9 mm toward NAD. The optimum pH of the enzyme activity is between 8.0 and 9.0, and the optimum temperature isaround ... investigated the catalytic proper-ties of HpSDH and the effects of pH and temperatureon HpSDH. The results show that HpSDH has a kcat of 7.7 ± 0.9 s)1, Km of 0.148 ± 0.028 mm and kcat⁄ Km of 5.2 ... the pH proﬁle of HpSDH. It is found that the pH optimum of HpSDH is between 8.0 and 9.0, and the pH optimum of AfSDH is between 7 and 7.5 [27]. Both AfSDH and HpSDH exhibit very low activities at...

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... (without the addition of ﬁbroblast homogenate) is applied to the HPLC-system the same picture appears. Thus, it seems, that the fronting, the shoulder and the tailing is due to acetyl-CoA and not ... toany other compound. (B) The peaks 3, 4, and 5 were isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 mM of the respective isomers of 3-MG-CoA in a total volume of 25 ... substrate [15]. The gene encodingthis bifunctional protein was named AUH (‘AU bind-ing homolog of enoyl-CoA hydratase’). The RNA-binding activity of the human protein and also of the murine homologue...

Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc

... (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP and DMAPP. The same is true for the genome of the archaeonN. equitans. No putative orthologs of the type II isomerasewere ... accompaniedby the appearance of three new13C-coupled signals at143.4, 111.3 and 21.4 p.p.m. assigned as the carbon atoms3, 4, and 5 of IPP, respectively (cf. Table 1 and Fig. 6).Within the limits of ... alignment of the amino acid sequences of 76 Idi-2 proteins.Gaps were removed from the alignment, and the total number of positions taking intoaccount was 320. The numbers at the nodesare the statistical...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... products of the hazelnutLOX were compared with the reaction products of soybeanLOX type I and authentic standards of 9- and 13-HODE.As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and can affect their availability inside the plant ... position 4235 of the genomic clone as the translation start. The characterization of other clonesrevealed the presence of another cDNA (CaLOX2) sharinghigh identity, 91.9%, with the CaLOX1. The comparisonof...

Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modiﬁcation sites and structural requirements for substrate processing and subcellular localization pptx

... process-ing of ubiquitin and ubiquitin-like proteins. They areresponsible for the disassembly of ubiquitin chains, and for the cleavage of mono- and oligomers of this mole-cule, either in precursor ... proteolysis of USP7 variants in the presence and absence of ubiquitin. SDS ⁄ PAGE (4–20% gradient gels) showing the limited proteolysis of native USP7-FL and variants thereof by trypsinover time with and ... in the oligomerization event.Substrate speciﬁcity and enzymatic properties of USP7As part of the characterization of USP7 biochemical properties, we have measured its kinetic parameters forthe...

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