Báo cáo khoa học: Biochemical and enzymological aspects of the symbiosis between the deep-sea tubeworm Riftia pachyptila and its bacterial endosymbiont pptx

... specific exhibition of a four-band pattern was observed. The two lower bands represent the normal and mutated homoduplexes and, the upper bands correspond to the two types of the normal ⁄ mutated ... activity), further investigation of the contribu- tion of the mutation p.Gln204Lys was required. Given the extremely low residual activity of most of the mutant pr...

Ngày tải lên: 23/03/2014, 04:21

... Apart from the obvious nonlinear correlation between C and F (the one is the reciprocal of the other), the correla- tions differed considerably between activation measures, PREPs and FREPs, and crosstalk ... correla- tion between the integral and the maximum (mean r S ¼ 0.9 ± 0.1) and a medium correlation between t M and s (r S ¼ 0.3 ± 0.2 and r S ¼ 0.7 ±...

Ngày tải lên: 30/03/2014, 10:20

... components of the MVB sorting machinery interact with Vps4p via the N-terminal MIT domain To determine whether there is any correlation between the binding sites on Vps4p and the response of the interacting ... conserved between the yeast and human VPS4 isoforms. In the Vps4p–IRA mutant, residues 56–71 in the third helix of the MIT domain were dele- ted. These...

Ngày tải lên: 23/03/2014, 09:20

... 1AxoFenon2AxoF3AxoFenon PαBE/CS S PβBE/CSS 1AxoFSS 2AxoFSS 1AxoF Rat-1 FTO-2B SS 1AxoF Fig. 1. Expression of CPS and its regulating transcription factors in Rat-1 and FTO-2B cells. (A) CPS and ... the lower strand. FoxA binding resulted in protection of the gua- nines at positions 347 and 351 on the upper strand, and protection at position 352 on the lower strand. Thes...

Ngày tải lên: 23/03/2014, 10:20

... Minic and G. Herve ´ (Eur. J. Biochem. 271) Ó FEBS 2004 REVIEW ARTICLE Biochemical and enzymological aspects of the symbiosis between the deep-sea tubeworm Riftia pachyptila and its bacterial endosymbiont Zoran ... specific and obligate nature o f t he symbiosis between vestimentiferans and their bacterial endosymbiont raises the question of...

Ngày tải lên: 30/03/2014, 15:20

... k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm toward NAD. The optimum pH of the enzyme activity is between 8.0 and 9.0, and the optimum temperature is around ... investigated the catalytic proper- ties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9...

Ngày tải lên: 19/02/2014, 05:20

... (without the addition of fibroblast homogenate) is applied to the HPLC-system the same picture appears. Thus, it seems, that the fronting, the shoulder and the tailing is due to acetyl-CoA and not ... to any other compound. (B) The peaks 3, 4, and 5 were isolated by HPLC and used as substrates for AUH. The AUH assay contained 2 m M of the respective isomers of 3...

Ngày tải lên: 19/02/2014, 07:20

... (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP and DMAPP. The same is true for the genome of the archaeon N. equitans. No putative orthologs of the type II isomerase were ... accompanied by the appearance of three new 13 C-coupled signals at 143.4, 111.3 and 21.4 p.p.m. assigned as the carbon atoms 3, 4, and 5 of IPP, respectively...

Ngày tải lên: 19/02/2014, 13:20

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and...

Ngày tải lên: 21/02/2014, 00:20

... process- ing of ubiquitin and ubiquitin-like proteins. They are responsible for the disassembly of ubiquitin chains, and for the cleavage of mono- and oligomers of this mole- cule, either in precursor ... proteolysis of USP7 variants in the presence and absence of ubiquitin. SDS ⁄ PAGE (4–20% gradient gels) showing the limited proteolysis of native USP7-FL and...

Ngày tải lên: 07/03/2014, 05:20