Báo cáo khoa học: Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor ppt

... report [33], and at least 20 pmol/10 6 cells for the H1 receptor (data not shown). Ligand affinities of the Sf9 cell expressed His-tagged H1 receptor were similar to those of the untagged receptor expressed ... pBacPAK9 encoding the human histamine H1 receptor (J E. Leysen, Janssen Pharmaceutica N.V., Beerse, Belgium) and of the cDNA encoding the...

Ngày tải lên: 30/03/2014, 14:20

... xamine whether modification of Cys157 and other P. falciparum- specific residues in the vicinity of the active site leads to irreversible inactivation of Pfen. Comparative studies on the structural and ... polymerase and 2 lL of the template library in a final volume of 20 lL. The amplified enolase PCR product and the pQE30 plasmid vector were digested with KpnIandPs...

Ngày tải lên: 16/03/2014, 18:20

... vascu- lar tissue of cotyledons and mature leaves, whereas, in the flowers, the anthers, connectives and filaments and the base and tip of the stigmata were stained. Similar to BPM2, BPM3 and BPM5 promoter:GUS ... cotyledons and in the central cylinder of the root, but not in the lower parts of the hypocotyl (a). Rosette leaves were stained in the vascular...

Ngày tải lên: 18/02/2014, 06:20

... in exploring the regulatory behavior of this enzyme. To avoid misinterpretation of the results, the composition and stoichiometry of the bound cofactor(s) and the stability of the dimeric structure of ... efficien- cies. The nonidentical nature of the Michaelis–Menten relationships of inhibitor-free and native epimerase is an indication of the regulatory...

Ngày tải lên: 16/03/2014, 00:20

... the light of studies of the structure and dynamics of the interaction between MeCP2 and methylated DNA at the molecular level. At the level of brain physiology, the unexpected reversibility of ... carbohydrate specificity, affinity and atomic details of interaction between the two P. aeruginosa lectins and their ligands allowed for the design and synthesis...

Ngày tải lên: 16/03/2014, 01:20

... Reversal of Bcl-2-mediated resistance of the EW36 human B-cell lymphoma cell line to arsenite- and pesticide-induced apoptosis by PK11195, a ligand of the HSS and mitochondrial permeability transition ... decreasing the activity of caspase-3 in the three cell lines. Effect of HSS knockdown on CCCP-induced apoptosis To further elucidate the functional role of HS...

Ngày tải lên: 16/02/2014, 09:20

... metabolism. Levels of cellular ceramide are regulated by the de novo pathway and the recycling pathway. The former relates to the synthesis of ceramide through the conden- sation of palmitate and serine ... together data suggesting that some of the genes involved in the genetics of Lewy body disease, have in common the fact that they impinge on ceramide metabolis...

Ngày tải lên: 18/02/2014, 14:20

... Le a determinant. As the C 3a ion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminal GlcNAc. The position of the second Fuc 3-linked to the internal ... function. One of the early demonstrations of the role of carbo- hydrates in recognition was binding of the influenza virus to red blood cells via sialic acid...

Ngày tải lên: 19/02/2014, 12:20

... (Radev and McKeown, 1997). It turns out that there is a large variety in the size of the profile (number of distinct descriptions) for different entities. Table 1 shows a subset of the profile ... manually and then validating them using the learning algorithm. However, the task proved (nearly) impossible considering the sheer size of the corpus. One possible ru...

Ngày tải lên: 17/03/2014, 07:20

... entirely to the catalytic role of these domains. Rather, the advantage appears to depend on the ability of the lipoyl group to protrude from the inner core of the complex, indicative of the biological ... Thus, the lipoate residues of the complex mediate the regulation of its first component by the last product of the overall reaction, NADH. Reduction of...

Ngày tải lên: 17/03/2014, 09:20