Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx

... tryptophan in the isolated FAD domain of rat nNOS (F1395W, F1395S and F1395A) and by probing the thermodynamic and kinetic consequences of these mutations. We have studied the thermodynamic and kinetic ... 1. The coenzyme-binding site in nNOS FAD domain and sequence alignments around the conserved aromatic residue in this domain. (A) The...

Ngày tải lên: 30/03/2014, 14:20

... best-performing results across the N-GRAM fea- tures and their combinations. In each case, our base- line is the majority class in the training set. 3.1 Subjectivity Among all the lemmatization settings, ... settings, a development setting (DEV) and a test setting (TEST). In the development setting, we run the typical 5 fold cross validation where we train on 4 folds an...

Ngày tải lên: 20/02/2014, 05:20

... subunit Serine ⁄ threonine protein kinase WNK4 Cellular organization Vimentin Lamin-A ⁄ C a-Actinin-2 a-Actinin-3 Desmin VASP Myosin-2 Myosin-3 Myosin-7 (myosin heavy chain 7) Myosin-7 Myosin-9 Myosin-9 Myosin-11 Myosin-13 ... from the column). On the other hand, members of extrinsic apoptosis pathways, such as caspase-8, Fas-associated death domain (FADD), and Bcl-2-interacting do...

Ngày tải lên: 06/03/2014, 22:21

... well and there is no example of mutational analysis of all of these residues in the same enzyme. To obtain an insight into how the many conserved residues in the active sites of family 18 chitinases contribute ... highlighting the residues discussed in this report. In the free enzyme, Asp142 is in the ÔdownÕ position, interacting with Asp140. Upon substrate-b...

Ngày tải lên: 07/03/2014, 14:20

... hydrolysis of the nucleotide. To investi- gate the in uence of the binding and ⁄ or hydrolysis of ATP on the interaction of the AAA-peroxins, Pex1p and Pex6p carrying mutated ATP-binding sites ... do interact in vivo. We assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for their inte...

Ngày tải lên: 07/03/2014, 16:20

... demonstrated the ‘sensitivity’ of the proximal region in determining the electronic and structural properties of the haem pocket. In the present study we attempt to obtain further insights into the ... comparison of the electronic absorption and RR spectra of the wild type and Thr171Ser either in the presence of the aromatic donor BHA or at alkaline...

Ngày tải lên: 07/03/2014, 21:20

... that the thiol group of Cys135 adds to C6 of the pyrimidine ring, favoring the exit of the aminic group. The subsequent addition of a water molecule and the expulsion of the active cysteine complete ... complete the reaction. Asp44 is positioned to stabilize and orient the binding of the substrate, and Tyr112, Glu205 (207 in HP-TenA) and Tyr47 assist...

Ngày tải lên: 16/03/2014, 00:20

... amino acid sequence. In particular, three cysteine residues and one serine residue in the cysteine cluster region, which co-ordinate the iron or cobalt ion of the a subunit, and two arginine residues ... accommodate the alkyl chain or aromatic ring of a nitrile substrate. bTrp72 of the Co-type NHase from P. thermophila replaces the tyrosine residue of Fe-type...

Ngày tải lên: 16/03/2014, 16:20

... buried in the interior of the protein molecule, to the ionone ring of retinol would be expected. Inter- estingly, binding of retinol caused an increase of the emission of the single Trp residue, similar ... In addition to the protein peaks, they also contained IAEDANS or IANBD bands, indicative of covalent binding of the dyes [19]. Binding activities of Ag-N...

Ngày tải lên: 16/03/2014, 18:20

... motif, actin-binding repeats, kech domains and ion channel motifs [10]. Proteins containing the POZ domain and zinc finger motif are termed POZ-ZF or POK proteins. Via the POZ domain, many POK proteins ... Long Xu and Peitang Huang Laboratory of Protein Engineering, Beijing Institute of Biotechnology, China The poxvirus and zinc finger (POZ) domain, formerly termed the b...

Ngày tải lên: 30/03/2014, 04:20