... ARPE -19 cells, indicating that Bach1 is not a sole determinant for HO -1 mRNA expression in ARPE -19 cells. Likewise, hypoxia induced expression of HO -1 and Bach1 mRNAs in D407 cells, indicating ... expression of HO -1 in human RPE cell lines In contrast to interferon-c, hypoxia indu ced the expression levels of HO -1 mRNA in D407 cells and exerted no or...
Ngày tải lên: 23/03/2014, 13:20
... 4h 8h 16 h 24h 1h 4h 8h 24h hA rA -p-p38 ratio 1. 0 2 .1 3.7 1. 9 1. 2 0.5 RINm5F Fig. 1. Western blot analysis of p-p38 kinase protein in RINm5F and CM cells. Total cell extracts were prepared from cells ... 206 , 11 07 11 15. 26 Xia Z, Dickens M, Raingeaud J, Davis RJ & Greenberg ME (19 95) Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... Activation of p 21- activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells In Duk Jung 1 , Jangsoon ... cells, cells were pretreated with either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059. Inhibition of tyrosine k...
Ngày tải lên: 30/03/2014, 13:20
Tài liệu Báo cáo khoa học: Activation of the Torpedo nicotinic acetylcholine receptor The contribution of residues aArg55 and cGlu93 ppt
... model of the cholinergic ligands-binding sites. J Biol Chem 265, 10 430 10 437. 14 Cohen JB, Sharp SD & Liu WS (19 91) Structure of the agonist-binding site of the nicotinic acetylcholine recep- tor. ... protein reveals the ligand-binding domain of nicotinic receptors. Nature 411 , 269–276. 11 Kao PN, Dwork AJ, Kaldany RR, Silver ML, Wid- eman J, Stein S & Karlin A (19 84)...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... M 2 (N-D)I3del:: Ga i1 (5.0 · 10 )8 m for atropine, and 3.3 · 10 )4 m for carbamylcholine) [25]. These results indicate that M 2 in the GOA -1 fusion protein is functional, and the ligand-binding properties ... described previously. GTPcS-binding assay The binding reaction was performed in 10 0 lL of binding assay buffer (20 mm Hepes ⁄ KOH, pH 8.0, 1 mm EDTA, 16 0 mm...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Activation of Stat5 and induction of a pregnancy-like mammary gland differentiation by eicosapentaenoic and docosapentaenoic omega-3 fatty acids docx
... total Virgin 21. 0 ± 1. 2 2 .1 ± 0.2 8.0 ± 2 .1 0 92.0 ± 8 .1 Pregnant 7.2 ± 1. 4 1. 0 ± 0.2 19 .2 ± 3.9 18 .2 ± 3.5 62.6 ± 7.8 Control 445.8 ± 89 .1 25.2 ± 3 .1 3.9 ± 0.2 0 96 .1 ± 8 .1 Fat -1 116 .5 ± 10 2 .1 ± ... during pregnancy. Cellular FABP com- prise a well-established family of cytoplasmic hydro- phobic ligand binding proteins and are involved in binding and i...
Ngày tải lên: 16/03/2014, 10:20
Báo cáo khoa học: Activation of hepatocyte growth factor activator zymogen (pro-HGFA) by human kallikrein 1-related peptidases docx
... Panc1, MiaPaCa), five human lung cancer cell lines (HLC1, LC-2, LC -1, T3M 11, LU139), two human renal cell carcinoma cell lines (MRT -1, Caki -1) , three human prostate cancer cell lines (LNCap, PC3, DU145), the ... cancer RCC Prostate cancer KLK2 KLK3 KLK4 MET KLK5 HGF/SF HGFA HAI -1 GAPDH HCT 116 MiaPaCa Colo205 RCM -1 HT29 LU139 DU145 LNCap DLD -1 SW837 Caco-2 LC-2 Panc1...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Activation of PMCA by calmodulin or ethanol in plasma membrane vesicles from rat brain involves dissociation of the acetylated tubulin/PMCA complex pdf
... (10 00 g for 5 min at 4 °C) and resuspended to a density of 1 · 10 9 cells mL )1 in buffer A containing 10 lm Fura-2AM. The suspension was incubated for 30 min at 30 °C with mild agitation. Cells were ... results indicate that acetylated tubulin and PMCA form part of the same complex inserted into PMVs and that PMCA4 is one of the isoforms present in the comple...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Activation of crystalline cellulose to cellulose IIII results in efficient hydrolysis by cellobiohydrolase pot
... min. K ad1 and K ad2 are expressed as lM )1 , A 1 , A 2 , and A max as nmolÆ(mg cellulose) )1 , and A 1 ÆK ad1 and A 2 ÆK ad2 as mlÆ(mg cellulose) )1 . K ad1 K ad2 A 1 A 2 A max A 1 ÆK ad1 A 2 ÆK ad2 Cellulose ... at 30 °C. This figure shows adsorption of Cel7A after incubation for 12 0 min as representative results of four time points (12 0, 18 0, 240, and 320...
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... mapping. Biochemistry 5, 415 6– 416 3. 21 Bradford MM (19 76) A rapid and sensitive method for the quantitation of microgram quantities of protein util- izing the principle of protein-dye binding. Anal ... three independ- ent experiments. Experiment Additions and 30 min of incubation Additions and 30 min of further incubation HAT (% of control) H + -ATPase activity (lmol...
Ngày tải lên: 23/03/2014, 11:20