Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc

... Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis Namthip Chumpolkulwong1, Chie Hori-Takemoto1, Takeshi Hosaka2, ... modiﬁcation of the ribosome, the apparatus of translation, in terms of high-yield protein production. Ourdevelopment of this ribosomal protein mutation is one of the strategies to enhance protein ... University of Tokyo, Bunkyo-ku, Tokyo, JapanWe examined the eﬀects of Escherichia coli ribosomal pro-tein S12 mutations on the eﬃciency of cell-free protein syn-thesis. By screening 150 spontaneous...

Báo cáo khoa học: Selection of full-length IgGs by tandem display on ﬁlamentous phage particles and Escherichia coli ﬂuorescence-activated cell sorting screening doc

... expression of soluble IgG, andPA-speciﬁc clones were identiﬁed by ELISA. Forty-nine of 192 of the screened clones gave a PA-speciﬁcsignal. Sequence analysis of 25 clones revealed the iso-lation of ... protectiveantigen of B. anthracis from a 1 : 108dilution of con-trol antibody. Such a dilution represents only 100 mem-bers of each speciﬁc clone in a library of 1010clones,which is near ... j constant domains, respec-tively, as a bicistronic operon downstream of the lac promoter. Center: map of fUSE5–ZZ phage for polyvalent display of the Fc-binding ZZ protein on all copies of...

Tài liệu Báo cáo khoa học: Effects of sequestration on signal transduction cascades docx

... sequestration in asimple covalent modiﬁcation cycle. Doc S1B. Amount of sequestration in covalent modiﬁ-cation cycles. Doc S1C. Models of double-phosphorylation andsequestration. Doc S1D. Model of ... concentrationand total kinase concentration, respectively, and KMisthe Michaelis–Menten constant of the kinase. The con-centration of the complex [TK] approaches the totalconcentration of ... expressedthe response of the active fraction to a change of thekinase concentration as a function of the concentra-tions of the two forms ([T] and [T*]) and the elastici-ties of the enzymes...

Báo cáo khoa học: Effects of a novel arginine methyltransferase inhibitor on T-helper cell cytokine production pot

... identiﬁcation of one such com-pound and the characterization its inhibitory proper-ties, focusing on its modulation of Th cell function.ResultsChemistryIn the report disclosing the activity of AMI-1, ... methylation reactions of recombinant Set7 ⁄ 9 with calf thymushistones as substrate and [3H]SAM in the presence of increasing concentrations of AMI-1 or compound 4. Data are representative of at ... IL-2 secretion [6]. Addi-tionally, arginine methylation regulates cytokine genetranscription in T helper (Th) cells through argininemethylation of the NFAT cofactor, NFAT interacting protein 45...

Báo cáo khoa học: "Effects of Word Confusion Networks on Voice Search" pdf

... Proceedings of the 12th Conference of the European Chapter of the ACL, pages 238–245,Athens, Greece, 30 March – 3 April 2009.c2009 Association for Computational Linguistics Effects of Word Confusion ... the effect of this improvement on thesearch task and demonstrate the beneﬁt of tightercoupling of ASR and the query parser on searchaccuracy.The paper outline is as follows. In Section 2, wediscuss ... extraction we canexplore for parsing a query. In the context of voicelocal search, users expect overall system responsetime to be similar to that of web search. Con-sequently, the relatively long...

Báo cáo khoa học: Effects of thymoquinone on isolated and cellular proteasomes pot

... TQ concentrations of 5 and20 lm, whereas it decreases at a concentration of 100 lm (corresponding to the recovery of proteasomeactivity). At the same time, increases in TQ concentra-tion resulted ... mutation consists of a single G ﬁ A transi-tion in codon 237, resulting in a missense mutation of methionine to isoleucine [32,33]. Interestingly, a studyconducted by Van Meir et al. on different ... the hydrolyzed AMCwas detected on a SpectraMax Gemini XPS microplatereader.Detection of proteasome–quinone adductsDetection of the TQ-mediated formation of quinoneadducts in isolated 20S immunoproteasomes...

Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deﬁciency pdf

... and the high degree of sequence conservation, we believe that the effects of these mutations on the structure and function of yeast enolase will be similar to their effects on humanbb-enolase. ... position would result ina change in the conformation of this loop. Changes inthe conformation of the backbone at this point andchanges in the orientation of other side chains, as aresult of ... °C, the signiﬁcantlylower yield of the variants may also be the result of dissociation followed by proteolysis of the monomers.The effects of these mutations on temperature stabil-ity is not...

Báo cáo khoa học: Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae potx

... K(2006) Evolutional design of a hyperactive cysteine-and methionine-free mutant of Escherichia coli dihydrofolate reductase. J Biol Chem 281, 13234–13246. Effects of salt on Vibrio endonucleases ... ithmeasurement of reﬂection h and <I(h)> is the weighted mean of allmeasurements of h.b5% of the reﬂections were used in theR-free calculations. Effects of salt on Vibrio endonucleases ... and extracellularproteins of marine organisms.The functional characterization of extremophilicproteins has so far focused on the obvious, i.e. the effects of temperature on thermophilic and...

Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin docx

... eﬀect of the mutations is related t o t he overall stability of T m a s a whole,and t hat the mutations have only m inor eﬀects on thecooperative interactions among proteins t hat c onstitute ... absence of troponin as a function of tropomyosinconcentration, and in the presence of troponin and Ca2+concentration varying from 10)6to 10)3M. InorganicÓ FEBS 2004 Cardiomyopathic mutations ... by varying the concen-tration of Tm in the presence of constant concentration of F-actin and myosin S1. In this condition, Tm inh ibits theATPase activity as its concentration increases [33]....

Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt

... the effects of 20E both on cell proliferation and morphological changes of IAL-PID2 cells.Isolation and characterization of P. interpunctellaEcR-B1mRNACloning of a PIEcR cDNA frament. We wonderedwhether ... tran-scription of PIEcR-B1, we stud ied t he effects of protein synthesis inhibitor, ANS on the induction of PIEcR-B1mRNA. The IAL-PID2 cells were cultured in Grace’smedium containing 20E ... ends of N-terminal region of the A/B domain. Using a B1isoform-speciﬁc probe from the A/B region of PIEcR, wedetected by Northern hybridization one transcript of 6 kb,closeinsizetothoseofDmEcR-B1...

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