Báo cáo khóa học: Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis doc

... Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis Namthip Chumpolkulwong 1 , Chie Hori-Takemoto 1 , Takeshi Hosaka 2 , ... modification of the ribosome, the apparatus of translation, in terms of high-yield protein production. Our development of this ribosomal protein mutation is one of the strategies to enhance p...

Ngày tải lên: 30/03/2014, 13:20

... expression of soluble IgG, and PA-specific clones were identified by ELISA. Forty- nine of 192 of the screened clones gave a PA-specific signal. Sequence analysis of 25 clones revealed the iso- lation of ... protective antigen of B. anthracis from a 1 : 10 8 dilution of con- trol antibody. Such a dilution represents only 100 mem- bers of each specific clone in a library of 10 10...

Ngày tải lên: 22/03/2014, 21:21

... sequestration in a simple covalent modification cycle. Doc S1B. Amount of sequestration in covalent modifi- cation cycles. Doc S1C. Models of double-phosphorylation and sequestration. Doc S1D. Model of ... concentration and total kinase concentration, respectively, and K M is the Michaelis–Menten constant of the kinase. The con- centration of the complex [TK] approaches the tota...

Ngày tải lên: 19/02/2014, 07:20

... identification of one such com- pound and the characterization its inhibitory proper- ties, focusing on its modulation of Th cell function. Results Chemistry In the report disclosing the activity of AMI-1, ... methylation reactions of recombinant Set7 ⁄ 9 with calf thymus histones as substrate and [ 3 H]SAM in the presence of increasing concentrations of AMI-1 or compound 4. Data...

Ngày tải lên: 06/03/2014, 11:20

... Proceedings of the 12th Conference of the European Chapter of the ACL, pages 238–245, Athens, Greece, 30 March – 3 April 2009. c 2009 Association for Computational Linguistics Effects of Word Confusion ... the effect of this improvement on the search task and demonstrate the benefit of tighter coupling of ASR and the query parser on search accuracy. The paper outline is as...

Ngày tải lên: 08/03/2014, 21:20

... TQ concentrations of 5 and 20 lm, whereas it decreases at a concentration of 100 lm (corresponding to the recovery of proteasome activity). At the same time, increases in TQ concentra- tion resulted ... mutation consists of a single G fi A transi- tion in codon 237, resulting in a missense mutation of methionine to isoleucine [32,33]. Interestingly, a study conducted by Van Meir et a...

Ngày tải lên: 15/03/2014, 11:20

... and the high degree of sequence conservation, we believe that the effects of these mutations on the structure and function of yeast enolase will be similar to their effects on human bb-enolase. ... position would result in a change in the conformation of this loop. Changes in the conformation of the backbone at this point and changes in the orientation of other side chai...

Ngày tải lên: 16/03/2014, 06:20

... K (2006) Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductase. J Biol Chem 281, 13234– 13246. Effects of salt on Vibrio endonucleases ... ith measurement of reflection h and <I(h)> is the weighted mean of all measurements of h. b 5% of the reflections were used in the R-free calculations. Effects of...

Ngày tải lên: 16/03/2014, 06:20

... effect of the mutations is related t o t he overall stability of T m a s a whole, and t hat the mutations have only m inor effects on the cooperative interactions among proteins t hat c onstitute ... absence of troponin as a function of tropomyosin concentration, and in the presence of troponin and Ca 2+ concentration varying from 10 )6 to 10 )3 M . Inorganic Ó FEBS 2004 Cardiom...

Ngày tải lên: 16/03/2014, 18:20

... the effects of 20E both on cell proliferation and morphological changes of IAL-PID2 cells. Isolation and characterization of P. interpunctella EcR-B1 mRNA Cloning of a PIEcR cDNA frament. We wondered whether ... tran- scription of PIEcR-B1, we stud ied t he effects of protein synthesis inhibitor, ANS on the induction of PIEcR-B1 mRNA. The IAL-PID2 cells were cultured...

Ngày tải lên: 23/03/2014, 13:20