Báo cáo khóa học: Puriﬁcation and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

... Puriﬁcation and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus Hisashi Nishiwaki, Katsuhiko Ito, Katsuhiko Otsuki, Hiroyuki Yamamoto, Koichiro ... tested by injectioninto cockroaches. Puriﬁcation of a toxic protein produced by B. cereus B. cereus was cultured aerobically in three steps to preparea large volume of the broth, from which one of ... The bacteria-free supernatantwas tested for its insecticidal activity against the cockroaches by injection.Identiﬁcation of B. cereus To identify the bacterial species producing insecticidal toxins,...

Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

... the modiﬁed CYP11B1 was produced by PCRusing the 5¢-primer (CGCCATATGGCTACTAAAGCTGCTCGTGTTCCACGTACAGTGCTGCCA) and 3¢-primer(GCGAAGCTTAATGATGATGATGATGATGGTTGATGGCTCTGAAGGTGAGGAG) and inserted ... Inouye K (2004) Puriﬁcation and characterization of mouse CYP27B1overproduced by an Escherichia coli system coexpress-ing molecular chaperonins GroEL ⁄ ES. Biochem BiophysRes Commun 323, 505–511.18 ... circular dichroism spectroscopic data. Nucl AcidsRes 32, W668–W673.22 Ro¨nnberg M, Ellfolk N & Soininen R (1979) Circulardichroism studies on cytochrome c peroxidase and cytochrome c- 551...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... Biology Sciences, Chinese Academy of Sciences, China3 Department of Chemistry, Renmin University of China, Beijing, China4 Research Institute of Pharmaceutical Chemistry, Beijing, ChinaConus ... supported by the National BasicResearch Program of China (2004CB719900), theNational Natural Science Foundation of China(20473013) and the Chinese Academy of Sciences forKey Topics in Innovation ... modiﬁcation of secreted geneproducts. Cell Mol Life Sci 62, 3067–3079.9 Montecucchi PC, de Castiglione R, Piani S, Gozzini L& Erspamer V (1981) Amino acid composition and sequence of dermorphin,...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... RTARAAYTGNCCNGCRTCYTGLP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGCCCAYTTLP7 AWAWALGWDDKLP8 GYHENALP9 WPLDYFLF2 GCCACACTTCTGTCAACATCC3RAC TTCTTCAAGGGCTGGCTCCCT3AP CTGATCTAGAGGTACCGGATCC5RACF ATCCTCACGAACAAGCAG5RACR ... ATCCTCACGAACAAGCAG5RACR GATCGCGATGCAGGCCTTFLF1 GGACGACTACAGCGTCTTCAGTAGAFLR1 TCCAAACAGTCAGTTTCTTAACCGTTable 1. Puriﬁcation of cellulase from abalone Haliotis discus hannai. Oneunitofcellulasewasdeﬁnedastheamountofenzymethatliberatesreducing ... the existence of a cellulase gene in abalonechromosomal DNA by genomic PCR.Correspondence to T. Ojima, Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences,Hokkaido...

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

... GAG CCC TGC GTG GC-3¢ and 5¢-AAC TCC GTG GAG AAG AAG AA-3¢ for theﬁrst PCR ampliﬁcation; and 5¢-TGC TGA CCG ACGCGC CTC CT-3¢ and 5¢-GGC AAC ACG GGC GTCACC GC-3¢ for the second PCR ampliﬁcation. ... N-terminalamino acid sequence of Mrcp-19k: 5¢-GTNCCN CCN CCN TGY GA-3¢ and 5¢-CAN CCY TTY TGYTTN ACY TT-3¢. The PCR products were resolved by 3%NuSieve 3 : 1 agarose (Takara) gel electrophoresis, and a53 ... 3¢-RACE was then carried out with a speci c 3¢-RACE primer designed from the 53 bp DNA and usinga3¢-RACE core kit (Takara). The 3¢-RACE primer usedwas 5¢-CTG ATC TAG AGG TAC CGG ATC CGT TCCCCC...

Báo cáo khoa học: Identiﬁcation and functional characterization of an aggregation domain in long myosin light chain kinase ppt

... were performed exactly asdescribed in the manufacturer’s manual.Construction of plasmids for L-MLCK variantsThe construction of chicken L-MLCK (pEGFP–MLCK210) and of its truncated variants tagged ... disrupted mitochondrial structure and functioningElectron microscopic images showed many aggregateparticles occupying mitochondria (Fig. 5A). In thesecases, most of the mitochondrial structures had ... there are twoMLCK genes at different genomic loci; those encodingskeletal muscle MLCK (skMLCK) and smooth muscleMLCK (smMLCK) [3]. smMLCK is the product of asingle gene, distinct from the gene...

Báo cáo khoa học: Puriﬁcation and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx

... electro-philic and genotoxic compounds by both catalytic activity and direct binding. In fact, the effect of these reactions is toconvert a reactive (lipophilic) molecule into a water-soluble(nonreactive ... substrates such as 1,2-dichloro-4-nitrobenzene,ethacrynic acid (ETHA), nitrobutyl chloride and D5-andro-stene-3,17-dione in conjunction with CDNB allows for amore complete biochemical characterization ... molecular mass and the GST-speci c enzymatic activities of puriﬁed GST isoforms of R. decus-satus. All puriﬁed isoforms catalyzed the conjugation of GSH with CDNB, whereas isoforms 1-1, 2-2 and...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... 516173SGAISWKTTVDTWSDEKKSYTCC GGC GCC ATC TCC TGG AAG ACG ACG GTG GAC ACG TGG AGC GAC GAG AAG AAG AGC TAC 576193HYGSNTCDQGKMCGHYTAVVCAC TAC GGC TCC AAC ACC TGC GAC CAA GGC AAG ATG TGC GGC CAC TAC ACC GCC GTC GTG ... 27693QEGYGSTQLPSDEPLNGLNDCAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336113KAIQDILNEHNMFRAKERVPAAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG ... GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 27693QEGYGSTQLPSDEPLNGLNDCAA...

Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... was ampliﬁed by PCR witholigonucleotides 5¢-CCCCATATGATGAGGACAGATTCATCAAAAATG-3¢ and 5¢-CATGGATCCTCATTTTTCTTCATTTTGAGGC-3¢. The ampliﬁed PCR fragmentwas inserted into plasmid vector pET between ... 1666 and 1247 cm)1bands are the characteristic frequencies of the random coil structure. Meanwhile, the 1682 and 1255 cm)1bands can be assigned to extended chain orb-turn structure [21].The ... resolution of spectrum was 1 cm)1. Protein concentration was 0.564mgÆmL)1in H2O.CD measurementsCD spectrum was acquired on a 0.1 cm path length cell of aJasco-715 spectrometer (Jasco, Tokyo,...

Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... template and two speci c primers For 6I 5¢-CGATGAATTCGTTAACGCTCATCACCATCACCATCACGGGAAATTGGCCATGGGGT-3¢ containing a HpaIsiteandBack6I 5¢-CGATGGTACCGTACTTGTTCATGCTTAGG-3¢ and subcloned into pUC19 ... sequence of EGT gene[32]. This 78 bp fragment was obtained after the annealing of the two following synthetic oligonucleotides For EGT 5¢-GATCCGCCACCATGACCATCTTATGTTGGCTCGCTCTCCTGAGCACACTCACAGCTGTTAACGCTGACATCA-3¢ ... of hST6Gal II lacking the ﬁrst 33 aminoacids of the open reading frame, was generated by PCRampliﬁcation using a 5¢ primer containing an EcoRI site,5¢-CCGACAGGAATTCCGCTGAGCCTGTACCCAGCTCCC-3¢...

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