Báo cáo khóa học: Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

... Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus Hisashi Nishiwaki, Katsuhiko Ito, Katsuhiko Otsuki, Hiroyuki Yamamoto, Koichiro ... tested by injection into cockroaches. Purification of a toxic protein produced by B. cereus B. cereus was cultured aerobically in three steps to prepare a large volume...

Ngày tải lên: 30/03/2014, 13:20

... the modified CYP11B1 was produced by PCR using the 5¢-primer (CGCCATATGGCTACTAAAGCTG CTCGTGTTCCACGTACAGTGCTGCCA) and 3¢-primer (GCGAAGCTTAATGATGATGATGATGATGGTTGAT GGCTCTGAAGGTGAGGAG) and inserted ... Inouye K (2004) Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpress- ing molecular chaperonins GroEL ⁄ ES. Biochem Biophys Res...

Ngày tải lên: 30/03/2014, 04:20

... Biology Sciences, Chinese Academy of Sciences, China 3 Department of Chemistry, Renmin University of China, Beijing, China 4 Research Institute of Pharmaceutical Chemistry, Beijing, China Conus ... supported by the National Basic Research Program of China (2004CB719900), the National Natural Science Foundation of China (20473013) and the Chinese Academy of Sciences for Key T...

Ngày tải lên: 18/02/2014, 17:20

... RTARAAYTGNCCNGCRTCYTG LP6 WAVEQMNYILGDNK R2 CATYTGYTCNACNGCCCAYTT LP7 AWAWALGWDDK LP8 GYHENA LP9 WPLDYFL F2 GCCACACTTCTGTCAACATCC 3RAC TTCTTCAAGGGCTGGCTCCCT 3AP CTGATCTAGAGGTACCGGATCC 5RACF ATCCTCACGAACAAGCAG 5RACR ... ATCCTCACGAACAAGCAG 5RACR GATCGCGATGCAGGCCTT FLF1 GGACGACTACAGCGTCTTCAGTAGA FLR1 TCCAAACAGTCAGTTTCTTAACCGT Table 1. Purification of cellulase from abalone Haliotis discus han...

Ngày tải lên: 20/02/2014, 23:20

... GAG CCC TGC GTG GC-3¢ and 5¢-AAC TCC GTG GAG AAG AAG AA-3¢ for the first PCR amplification; and 5¢-TGC TGA CCG ACG CGC CTC CT-3¢ and 5¢-GGC AAC ACG GGC GTC ACC GC-3¢ for the second PCR amplification. ... N-terminal amino acid sequence of Mrcp-19k: 5¢-GTN CCN CCN CCN TGY GA-3¢ and 5¢-CAN CCY TTY TGY TTN ACY TT-3¢. The PCR products were resolved by 3% NuSieve 3 : 1 agarose (Takara) gel...

Ngày tải lên: 16/03/2014, 05:20

... were performed exactly as described in the manufacturer’s manual. Construction of plasmids for L-MLCK variants The construction of chicken L-MLCK (pEGFP– MLCK210) and of its truncated variants tagged ... disrupted mitochondrial structure and functioning Electron microscopic images showed many aggregate particles occupying mitochondria (Fig. 5A). In these cases, most of the mitocho...

Ngày tải lên: 30/03/2014, 04:20

... electro- philic and genotoxic compounds by both catalytic activity and direct binding. In fact, the effect of these reactions is to convert a reactive (lipophilic) molecule into a water-soluble (nonreactive ... substrates such as 1,2-dichloro-4-nitrobenzene, ethacrynic acid (ETHA), nitrobutyl chloride and D5-andro- stene-3,17-dione in conjunction with CDNB allows for a more complete...

Ngày tải lên: 31/03/2014, 09:20

... 516 173SGAISWKTTVDTWSDEKKSY TCC GGC GCC ATC TCC TGG AAG ACG ACG GTG GAC ACG TGG AGC GAC GAG AAG AAG AGC TAC 576 193HYGSNTCDQGKMCGHYTAVV CAC TAC GGC TCC AAC ACC TGC GAC CAA GGC AAG ATG TGC GGC CAC TAC ACC GCC GTC GTG ... 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336 113KAIQDILNEHNMFRAKERVP AAG GCC ATA CAG GAC ATC CTC AAC GAG CAC...

Ngày tải lên: 07/03/2014, 12:20

... was amplified by PCR with oligonucleotides 5¢-CCCCATATGATGAGGACAGATT CATCAAAAATG-3¢ and 5¢-CATGGATCCTCATTTT TCTTCATTTTGAGGC-3¢. The amplified PCR fragment was inserted into plasmid vector pET between ... 1666 and 1247 cm )1 bands are the characteristic frequencies of the random coil structure. Meanwhile, the 1682 and 1255 cm )1 bands can be assigned to extended chain or b-turn structu...

Ngày tải lên: 07/03/2014, 15:20

... template and two speci c primers For 6I 5¢-CGATGAATTC GTTAACGCTCATCACCATCACCATCACGGGAAA TTGGCCATGGGGT-3¢ containing a HpaIsiteandBack 6I 5¢-CGATGGTACCGTACTTGTTCATGCTTAGG-3¢ and subcloned into pUC19 ... sequence of EGT gene [32]. This 78 bp fragment was obtained after the annealing of the two following synthetic oligonucleotides For EGT 5¢- GATCCGCCACCATGACCATCTTATGTTGGCTCG CTCTCCTGAG...

Ngày tải lên: 08/03/2014, 08:20