Báo cáo khoa học: Secretion of proteases in serglycin transfected Madin–Darby canine kidney cells ppt

... al. Serglycin and proteases FEBS Journal 273 (2006) 536–547 ª 2006 The Authors Journal compilation ª 2006 FEBS 547 Secretion of proteases in serglycin transfected Madin–Darby canine kidney cells Lillian ... sergly- cin, and amount of serglycin core protein as detected by western blotting, also showed the highest secretion of proteases. Transfection of sergl...

Ngày tải lên: 30/03/2014, 11:20

... new pieces of information, which were implicit in the "linear" input form, are made explicit in the result of the analysis. These pieces of information are extracted using the syn- ... 1983)) this book-keeping need not be completely explicit, but the interpreter of the language (usually a dialect of PROLOG) has to keep track of the binding of the variables,...

Ngày tải lên: 21/02/2014, 20:20

... explain their wide distribution in tissues and cells [16], including stem cells [17]. Increasing evidence links these noncatalytic actions with the binding of cholinesterases to several protein partners. ... extent of binding with the lectins concanavalin A, Lens culinaris agglutinin (LCA), and Ricinus communis agglutinin (RCA) (Fig. 3), ruled out the blood origin and supported the...

Ngày tải lên: 06/03/2014, 22:21

... INTERPRETATION: posted earnings; 7. ANECDOTES; 8. OTHER: poems. The goal of our research is to identify the role of verbs, keeping in mind that event profile is but one of many factors in ... still convinced that our initial hypothesis on the role of verbs in determining event profile was worth pursuing. We believe that these results are a by-product of lexical ambigui...

Ngày tải lên: 08/03/2014, 05:21

... to interfere with the attachment of glycosaminoglycan chains to their respective protein cores. Activation of the J774 macrophages with PMA resulted in increased secretion of trypsin-like serine ... the secretion of proteoglycans in these cells is linked to in ammatory reactions and that its function(s) may be linked to the binding, transport and regulation of other secre...

Ngày tải lên: 08/03/2014, 08:20

... in intact cells [5]. We therefore deci- ded to study the binding of the SEC14-containing p45 to phospholipids using a filter-binding assay [17]. Both natural purified and recombinant p45 proteins ... fluorescent protein; GOLD, Golgi dynamics; HA, hemagglutinin; PH, pleckstrin homology; PI3K, phosphatidylinositol 3¢-kinase; PITP, PtdIns transfer proteins; PtdIns, phosphatidylinositol; PTP,...

Ngày tải lên: 16/03/2014, 23:20

... are single-polypeptide cytoplas- mic proteins composed of an N-terminal ATPase domain, a substrate-binding region, and a C-terminal 15 kDa ‘lid’ regulating binding affinity for ‘client’ pro- teins. ... on the binding of wt-mNBD1 and F508del-mNBD1 to immobilized Hsc70. As shown by the solid lines in Fig. 5A,B, increasing concentrations of ATP caused a dramatic reduction in the binding...

Ngày tải lên: 23/03/2014, 05:22

... the intensities of the band of intact protein at the beginning of tryp- sinolysis and at the fixed time of trypsinolysis) against the time of incubation (Fig. 8D). The apparent rate constants of ... shock proteins, each of which contains the so-called a-crystallin domain consisting of 80– 100 residues and located in the C-terminal part of the molecule. The a-crystallin doma...

Ngày tải lên: 23/03/2014, 07:20

... +0.708 V, in comparison with spectra obtained in the presence of a 2- or 10-fold molar excess of stigmatellin. Upon binding of stigmatellin, shifts reflecting the changes within the binding site ... monitoring of the protonation state of several crucial residues in the presence and absence of stigmatellin. Interestingly, several residues perturb the orientation of the quino...

Ngày tải lên: 23/03/2014, 10:20

... However, in support of calpains acting as secretases is the fact that these proteases are involved in cleavage of other membrane surface pro- teins, including growth factor receptors, integrin and glutamate ... tangles [32]. Calpastatin, a specific calpain inhibitor, is altered in AD. Indeed, it decreases as the number of plaques and tangles increase in AD brains [33]. Moreover...

Ngày tải lên: 23/03/2014, 10:21