Báo cáo khoa học: Structures for amyloid fibrils doc

... and L. C. Serpell Structures for amyloid fibrils FEBS Journal 272 (2005) 5950–5961 ª 2005 The Authors Journal compilation ª 2005 FEBS 5961 MINIREVIEW Structures for amyloid fibrils O. Sumner Makin ... highly detailed structures for amyloid fibrils formed from cer- tain peptides [43,75,101,103] (Fig. 3). The evidence for these structures appears to clearly exclud...

Ngày tải lên: 30/03/2014, 11:20

... Ab fibrils, we performed the same set of experiments on fibrils formed from another amyloid forming protein, non- amyloid- b-component (NAC) [12]. CLAC was found to have a similar effect on NAC fibrils, ... how the amyloid plaques are formed. In vitro studies indicate that unstructured Ab mono- mers spontaneously form soluble oligomers (seeds), which, in turn, form protofibrils an...

Ngày tải lên: 30/03/2014, 16:20

... solvation of backbone atoms is much larger for a conformers than for b conformers. Interaction with solvent thus compensates for the destabilization of the a conformation as a result of peptide dipole moments. ... switches to what is occurring in the early steps of amyloid- fibril formation. For many systems, including the stefins [124–126], amyloid- fibrils form at nonphysiologica...

Ngày tải lên: 14/02/2014, 18:20

... Mayrose I, Bacharach E & Pupko T (2005) Selecton: a server for detecting evolutionary forces at a single amino-acid site. Bioin- formatics 21, 2101–2103. 39 Da Lage JL, Danchin EG & Casane ... proteins 3D struc- tures. Bioinformatics 18, 1250–1256. 57 Shatsky M, Nussinov R & Wolfson HJ (2004) A method for simultaneous alignment of multiple protein structures. Proteins 56, 143...

Ngày tải lên: 18/02/2014, 13:20

... RT-PCR analysis was performed as described previously. The expected sizes of the PCR products were 1270 bp for HVEM, 738 bp for nectin-1, 616 bp for nectin-2, and 736 bp for 3-OST-3, respectively. ... phalloidin (Invitrogen) was added for F-actin staining at room temperature for 45 min. Finally, the cells were washed three times with 1· NaCl ⁄ P i before micros- copy was performed...

Ngày tải lên: 18/02/2014, 14:20

... these papers clearly show domain-specific binding for M 2+ (M ¼ Zn, Cd) initially to the a domain, thereby lead- ing to formation of the M 4 a cluster prior to formation of the M 3 b cluster, demonstrating ... in independent tetra- thiolate sites, the choice for the third incoming metal ion would be to form a bridging interaction in the a domain or to form another independent tetrathiolate...

Ngày tải lên: 19/02/2014, 00:20

... vitro systems as supportive medical care and for sympto- matic treatment; they represent an important aid for patients and for improving survival in severe forms of disease. Antiviral therapeutic ... conclusion, considerable effort has recently been made towards inhibiting the viral replication of DENv. Much remains to be performed to achieve results suit- able for experimentation in...

Ngày tải lên: 19/02/2014, 00:20

... maintained tetrameric structures. In vitro fibril formation of TTR was performed to compare the effects of amino acid side chain oxidation. Structural transitions of proteins to amyloid fibrils can be ... kinetics of fibril formation of these oxidized proteins reveal the amino acid inter- actions that are critical in the onset of amyloido- genesis. The rates of amyloid fibril formation...

Ngày tải lên: 19/02/2014, 05:20

... frames using PCR. For the first potential glycerol dehydrogenase gene, we predicted introns in the genomic DNA. For that rea- son, we amplified the open reading frame from cDNA. For the second of ... The tagged protein was purified and then used for further analysis. In the reductive reaction, the Michaelis–Menten con- stant K m for DHA was 1 mm, and the K m for NADPH was 50 lm. The...

Ngày tải lên: 19/02/2014, 06:20

... indicated above the lanes) were assessed using export assays for TorA (upper panel) or TorA-GFP (lower panel) as described earlier for other mutants (Fig. 2). Mutagenesis of TatABC C. M. L. Barrett ... essential for Tat function. Taking these previ- ous reports into consideration, the logical conclusion is that several residues (F20, G21 in TatA, G21, P22, P26 in TatB) are probably impo...

Ngày tải lên: 19/02/2014, 17:20