Báo cáo khoa học: Probing protein–chromophore interactions in Cph1 phytochrome by mutagenesis potx

... et al. Protein–chromophore interactions in Cph1 FEBS Journal 273 (2006) 1415–1429 ª 2006 The Authors Journal compilation ª 2006 FEBS 1429 Probing protein–chromophore interactions in Cph1 phytochrome ... impotent. In oat phytochrome A the phytochromobilin (PFB) chromophore is attached by a thioether link to C322 #380 [11–13], a residue conserved in plant-type phytoc...

Ngày tải lên: 30/03/2014, 11:20

... ainly c ause d by t he faster unfolding resulting from destabilization of the native state relative to the transition state, underlining the predominant importance of this region for maintaining ... DDG [D]50% and DDG # U 25  C shows that the decrease in the thermodynamic stability is mainly caused by an increase in the unfolding rate constant, i.e. a decrease in the kinetic stabil...

Ngày tải lên: 16/03/2014, 18:20

... lactacystin [27] were analysed by Western blotting. As can be seen in Fig. 3A, accumulation of the 32-kDa protein, corresponding to the single-chain aD11-sec, was induced by treatment with lactacystin ... obtained to date indicate that inhibition of Ras activity suppresses cell proliferation and induces regression in a broad range of tumors. Intracellular antibodies, in particular s...

Ngày tải lên: 21/02/2014, 00:20

... droplets surrounded by adipophilin (Fig. 4B) and by Oil Red O staining (Fig. 5E,F). In cells cultured in the absence of VLDL (control cells), nominal diffuse cytoplasmic adipophilin staining was observed ... we incubated THP-1 macrophages with increasing concentrations of VLDL and examined adipophilin levels by immuno- blotting (Fig. 3). In the presence of 10 and 100 lgÆmL )1 VLDL...

Ngày tải lên: 23/03/2014, 10:21

... are intro- duced in the text. The existing approach for correcting the spelling error in the languages that have no word boundary assumes that all substrings in input sentence are error strings, ... words in text is harder than in English because of additional ambiguities in locating er- ror words. The traditional method handles this by hypothesizing that every substrings...

Ngày tải lên: 23/03/2014, 19:20

... features. In Proceedings of COLING-ACL-2006. Zhu Zhang. 2005. Mining Inter-Entity Semantic Relations Using Improved Transductive Learning. In Proceedings of ICJNLP-2005. Shubin Zhao and ... Extracting Relations with Integrated Information Using Kern el Methods. In Proceedings of ACL-2005. Guodong Zhou, Jian Su, Jie Zhang and Min Zhang. 2005. Exploring various knowledge in rela...

Ngày tải lên: 24/03/2014, 03:20

... ssDNA binds to the protein via extensive electrostatic interactions and stacking contacts. The rest of RPA70, as well as its DNA-binding domain, interact with protein partners that are involved in DNA ... a role in determining the binding affinity of XPC–hHR23B for DNA. Furthermore, it is known that DNA bending is induced by UV-DDB binding to damaged DNA sites. Taken together, these fin...

Ngày tải lên: 07/03/2014, 12:20

... mine the identity and location of the nucleotide insertion. Four mutants obtained contained in- frame insertions resulting in the insertion of five amino acids into the Cdc37 protein. Stop ... FEBS Cdc37 maintains cellular viability in Schizosaccharomyces pombe independently of interactions with heat-shock protein 90 Emma L. Turnbull, Ina V. Martin* and Peter A. Fantes Institute of ......

Ngày tải lên: 16/03/2014, 22:20

... mutating the corresponding resi- dues in E. coli FabG involved in the binding of E. coli ACP have been investigated earlier, the number of binding sites and ACP-induced cooperativity for the interactions ... conformational changes by monitoring the change in its intrinsic fluorescence. In these studies, we have investigated the interactions between PfFabG and PfACP using a combin...

Ngày tải lên: 23/03/2014, 10:20

... and its interaction with nephrin has been examined also in vitro, although the mapping of interacting domain for nephrin binding resulted in controversial reports [27,28]. Studies employing gradient ... protein–protein interactions of the nephrin intracellular domain, we produced a series of recombinant nephrin proteins. These were able to bind all previously identified ligands, although...

Ngày tải lên: 23/03/2014, 13:20