Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx

... FEBS Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells K. Rathinasamy and D. PandaSchool of Biosciences and Bioengineering, ... the suppression of microtubule dynamics by benomyl was the cause for the loss of tension across the kinetochores and activation of the checkpoint pro-teins and induction of apoptosis. Results Benomyl ... intomicrotubules and suppresses dynamic instability of reconstituted bovine brain microtubules in vitro [10] . Benomyl binds to mammalian tubulin with a moderateafﬁnity and the binding of benomyl to tubulin...

Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... levels, and sLeaexpression in cancer cell lines and recombinant clonesWe also measured the levels of b3Gal-T5 transcript and b1,3Gal-T activity in different cancer cell lines and clones, and compared ... formed in metabolically radiolabelled cells. The main radioactive oligosaccharidesformed in BxPC3 cells (j in lower part, and A, B, and C of upper part)andT5ASclone(h in lower part, and A, B, and ... various abdominalillnesses [32] including cancers of the digestive tract [33–35].Moreover, it is an E-selectin ligand [36] and may be involved in the metastatic spread of cancer cells, as suggested...

Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf

... termination, and recy-cling. Initiation is the rate-limiting step [1]. This phaseis mediated by initiation factor 1 (IF1), initiation fac-tor 2, and initiation factor 3. IF1 is the smallest of ... substrates for the binding of ribo-somal proteins and maturation factors [13]. Amongthese structures are double-stranded stems that arecomposed of terminal ﬂanking sequences of 16S and 23S rRNAs. ... 23 °C in a water bath, and then grown at 23 °C to log phase. Cells were chilled, and harvested by centrifugation at 4000 g for 15 min. The cells were destroyed by energetic rubbing, using Al2O3mixedwith...

Báo cáo khoa học: Suppression of NADPH oxidase 2 substantially restores glucose-induced dysfunction of pancreatic NIT-1 cells doc

... of ROS production in pancreatic b -cells [6]. Thoseauthors found suppression of high glucose-inducedROS production and decreased glucose-stimulatedinsulin secretion by DPI in cells of the insulin-producingcell ... USA).Determination of apoptosis occurrenceTo assess the occurrence of apoptosis in NIT-1 cells, cells were double-stained with Annexin V and a PI kit (Baosai,Beijing, China) according to the ... whether d-glucose induces apopto-sis of NIT-1 cells. NIT- 1cells were doubly stainedusing Annexin V and PI kit. Annexin V can combinewith the phosphatidylserine on the surface of the cellu-lar...

Báo cáo khoa học: Suppression of urokinase receptor expression by bikunin is associated with inhibition of upstream targets of extracellular signal-regulated kinase-dependent cascade pptx

... Terao,T. (2001) Suppression of urokinase expression and invasiveness by urinary trypsin inhibitor is mediated through inhibition of proteinkinase C- and MEK/ERK/c-Jun-dependent signaling pathways.J. ... Terao,T. (2001) Structure and function analysis of urinary trypsininhibitor (UTI): identiﬁcation of binding domains and signalingproperty of UTI by analysis of truncated proteins. Biochim.Biophys. ... with intactbikunin or bikunin derivatives is shown in Fig. 6 and Table 1. Exposure of cells to bikunin, bikunin-a and bikunin-n resulted in decrease in the amount of ERKphosphorylation in cell...

Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot

... mL) and incu-bations continued in fetal bovine serum-free culture mediumfor 24 h in the presence of ApoA-I ⁄ phosphatidylcholine(100 lgÆmL)1). At the times indicated in the text, aliquots of the ... 0.001) (Fig. 1). Inhibition of NF-jB transcrip-tional activity by CRLPs containing trilinolein ortriacylglycerol from corn oil was conﬁrmed by mea-suring luciferase activity in cells transfected ... (Fig. 2).Effects of CRLPs on cytokine and chemokinesecretion and mRNA expression in macrophagesWe initially examined the effects of CRLPs on therelease of TNFa, IL-6, IL-1b and MCP-1, which...

Báo cáo khoa học: Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inﬂammatory drugs potx

... levels of the expression of hsps differed depending on the drug.Induction of thermotolerance of cells by INDAs IND and NDGA caused hsps to accumulate in mammalian cells, we next examined whether ... is otherwiseinsufﬁcient. In contrast, IND induces the expression of Hsp70 in human umbilical vein endothelial cells [18] or induces the nuclear localiz ation of Hsc70 in mammalian cells [19]. ... constitutive isoform of Hsp70)increased, and t he expression of Hsp70 (an inducibleisoform of Hsp70) was markedly induced (Fig. 2B). Onthe other hand, the increase i n the levels of Hsp105a and Hsc70...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... performance of upper-levelresidents and other medical staff across a variety of disciplines. We likewise agree that optimizing patient hand-offs, medical education, and trainees’ sense of professionalism ... rapidly increases after 16 hours of sustainedwakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working formore than 16 hours on the ... Journal Club review [1] of theirstudies examining the effect of reducing interns work hours in the intensive care unit [2,3].Our statement that “interns in the traditional arm may havehad more...

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... bias in the selec-tion of recombination points. It has been suggestedthat optimal recombination points allow swapping of structural blocks [19], and combination of large pro-tein fragments in ... chimera,recombination preserved the integrity of domain D1 and the very end part of domain D3, two regions thatinteract precisely in the natural laccase fold (Fig. 5).Because of the bias introduced by linearization ... together, recombination inducedswapping of amino acids for 94 positions (19% of theresidues) in the original LAC3 sequence. The C- and N-termini of the hybrids were 33–37% and 9–26%different,...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... morphology of the strains used in this study. (A) Morphology of deletionstrains. The indicated strains were grown in YPD to stationary phase. The cells werethen ﬁxed with formaldehyde and examined by ... formation of a matingprojection [28]. Furthermore, homologues of oxysterol-binding proteins, a family of proteins that regulatethe synthesis and transport of sterols, were found toparticipate in ... beexplained by a negative regulation of Are2p activity by Cla4p. Alternatively, Are2p activity may be normal in cells lacking CLA4, and increased amounts of SE mightsimply be a consequence of higher...

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