Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx

... recognized blood group A-active glycoconjugates on the cell sur- face of undifferentiated [16] and differentiated HT29 cells (Fig. 4). Although no direct quantification of blood group A-active glycoconjugates ... Binding of Escherichia coli heat-labile enterotoxin (LT-I) to HT29 cells in culture. Differentiated and control HT29- ATCC (A) and HT29- 5F...

Ngày tải lên: 30/03/2014, 10:20

... character- ization of E. coli Pta and set out to investigate the function of its N-terminal domain by the construc- tion and analysis of three E. coli Ptas with deletions from the N-terminal ... PCR from plasmid pCA24N–Pta, hereafter called pPta, containing the entire coding sequence of pta from E. coli. Different sets of primers were used to amplify different E. coli...

Ngày tải lên: 06/03/2014, 11:20

... the pattern of splicing of E1A mRNA in comparison with empty pEGFP vector (Fig. 4B). Expression of EGFP– Ki-1 ⁄ 57 leads to formation of the 10S and 9S iso- forms, concomitantly with a reduction of 13S ... malig- nant cells from Hodgkin’s lymphoma. Despite studies showing its phos- phorylation, arginine methylation, and interaction with several regulatory proteins, the...

Ngày tải lên: 16/03/2014, 02:20

... only treatment of CTPS with trypsin produced a limited number of cleavage fragments over the course of 1 h, of which the formation of some fragments was suppressed in the presence of ATP and/ or ... and purification Wild-type and mutant forms of recombinant E. coli CTPS were expressed in and purified from E. coli strain BL21(DE3) cells transformed with either mutant or...

Ngày tải lên: 23/03/2014, 17:22

... the function of the RC gene of Escherichia coli. Nature 221, 838–841. 14. Cashel, M. & Kalbacher, B. (1970) The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a ... h )1 in both groups of media. Therefore, the growth rate of GRd.tsf ranges from  70– 95% of that of UY211 (Fig. 3). The lowest growth rate of GRd.tsf (70%), relativ...

Ngày tải lên: 21/02/2014, 00:20

... hydrogen-bonded with Pro216 and Tyr218 from the other subunit, and finally leading to the disintegration of tetramers of the enzyme. This can be fitted well with the explanation of why the E59L ... Hung HC (2006) Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 36...

Ngày tải lên: 23/03/2014, 06:20

... Authors Journal compilation ª 2009 FEBS The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity Luigi ... activity of SlyD is not required for maturation of Escherichia coli hydrogenase. J Bacteriol 189, 7942–7944. 25 Leach MR, Sandal S, Sun H & Zamble DB (2005) Metal bind...

Ngày tải lên: 30/03/2014, 01:20

... con- sumption of O 2 and substrate with the formation of detectable products. Figure S4 shows that conversion of 2,4-pentanedione (5 mm) proceeded with depletion of a molar equivalent of dissolved ... orbital with the 3d orbital of the metal cofactor. Two important pieces of information can be gleaned from the pre-edge peak. First, occurrence of this tran- sition impli...

Ngày tải lên: 18/02/2014, 06:20

... phos- photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo- retical and experimental points of view, and has Keywords Escherichia coli; model verification; ... model [14] Table 2. Comparison of the predictions of the specific glucose uptake by the model of [15] with the model of [18], and with the experimental results...

Ngày tải lên: 18/02/2014, 13:20

... particular, phosphorylation of dGuo was reduced markedly. Structure of Dm-dNK-E52D in complex with dTTP The structure of one of the mutants, E52D, was solved using X-ray crystallography at a resolution of 2.5 A ˚ in complex ... a structural study of Dm-dNK in which the enzyme was cocrystal- lized with both deoxythymidine (dThd) and dTTP sep- arately, E52 formed a hydrogen bond...

Ngày tải lên: 19/02/2014, 02:20