Báo cáo khoa học: Mechanical regulation of the Cyr61/CCN1 and CTGF/CCN2 proteins Implications in mechanical stress-associated pathologies pptx
Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
... box), explaining the regulation of desA expression by binding of DmdR1 to the iron box. The same overlapping was found in S. pilosus. DmdR1 binds to the promoter region of desABCD in both S. ... role of iron and the DmdR1 regu- lator in the transcriptional control of the desferrioxam- ine cluster. In this study, we report the deletion of the first gene...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... regulated independently by the main fuel sources in muscle and by insulin through directly altering the expression of the human PDK2 and PDK4 isoforms. In addition to our findings, insulin has been ... regulation of mRNA and protein expression, an increase in PDK4 protein abundance independent of A B C D Fig. 2. Semi-quantitative RT-PCR showing the effects of insu...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
... loop of the protein that might b e involved in electrostatic binding of the natural electron donor ferredoxin (M. Winkler, B. Neil & T. Happe, unpublished results). In the N-terminus of other ... that the proteins containing two [4Fe–4S] clusters a re ancestors of the Fe-hydrogenases [55]. The N-terminus of the C. reinhardtii and S. obliquus proteins is s...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt
... calculated using the forcefield of discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin. The position of mannose observed in the Man–banana lectin complex ... energy over the threshold value. The docking of MeMan into the carbohydrate-binding sites of Orysata and other JRLs was performed with the program insig...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... plots. Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by inductively ... from quinoline [1,2]. Besides quino- line, some quinoline derivatives and the benzodiazines quinazoline and quinoxaline are accepted as substrates [1,3]. Like othe...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... Unfolding and stability of Thermotoga maritima HU (Eur. J. Biochem. 271) 1507 Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic ... multiple 24-curve fitting of DSC data to the two-state model. The dashed lines show the confidence interval of the fitting. The results of the individ- ual curve fi...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx
... number of repeated domains; six in the case of gelsolin itself, adseverin and villin, and three in capG, fragmin and severin (for a review, see [2]). The binding of calcium to gelsolin and to actin bound ... to G2 domain. Footprint of G2 on gelsolin G4–6 domain Two sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... protec- tive roles of spermine in free radical scavenging and DNA shielding, and resulting in an overall increased potential for oxidative DNA damage in these cells. The results of these studies therefore ... components of the FAD-binding domain, and are therefore essential for catalysis [28,33–35]. Kinetic properties of SMO5 Polyamine oxidase activity of SMO5 and...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: "Transparent combination of rule-based and data-driven approaches in a speech understanding architecture" pot
... transcribed and an- notated the data using a simple Java-based tool, randomly selecting 75% of it for use in training and keeping the rest for testing. During the course of the project, we routinely ... follows: 3.1 Training and decoding Training data for ALTERF is supplied in the form of a text file containing one example per line, in the format 1. Initialise...
Ngày tải lên: 08/03/2014, 21:20
Báo cáo khoa học: Making sense of G-quadruplex and i-motif functions in oncogene promoters pot
... accessory protein or a minor recombinant protein [34]. Studies show that an R88A mutation (arginine to alanine) in the nucleotide- binding site eliminates binding of the NM23-H2 to single-stranded DNA. ... II NM23-H2 5′ 3′ 3′ 5′ OFF ABC E D 5′ 3′ 3′ 5′ OFF Fig. 5. Cartoon showing the involvement of NM23-H2, nucleolin and a G-quadruplex-interactive compound in modulating the...
Ngày tải lên: 23/03/2014, 03:20