Báo cáo khoa học: Characterization of a b-N-acetylhexosaminidase and a b-N-acetylglucosaminidase/b-glucosidase from Cellulomonas fimi potx

... 2004 Characterization of Mesorhizobium huakuii lipid A containing both D -galacturonic acid and phosphate residues Adam Choma 1 and Pawel Sowinski 2 1 Department of General Microbiology, Maria Curie-Sklodowska ... by galacturonic acid. Long chain fatty acids with a hydroxyl group at the (x-1) position are present in lipids A of almost all bacteria from the Rhizobiaceae [18...

Ngày tải lên: 07/03/2014, 15:20

... His 6 -PecA, His 6 -PecE, and His 6 -PecF His-tagged PecA, E and F were purified separately by metal ion chelating affinity chromatography on chelating seph- arose (fast flow; Amersham Pharmacia Biotech AB, according ... preparation of the subunits of PVB-PEC-lyase possessing His 6 -tags at the N terminus (to facilitate purification) and on their enzymatic char- acterization. MATERIALS...

Ngày tải lên: 21/02/2014, 03:20

... thio-b-d-galacto- side and polyethyleneimine plates (Macherey & Nagel, Du ¨ ren, Germany) were from Sigma-Aldrich (St. Louis, MO, USA). 3 H-labelled acylated tRNA was prepared and stripped of amino acid according ... Ni-NTA agarose beads, unbound protein was removed (lane 2) and the beads washed (lanes 3, 4). SF was eluted with imidazole (lane 5) and precipitated by dialysis a...

Ngày tải lên: 07/03/2014, 16:20

... obtained from Roche Molecular Biochemicals (rabbit muscle PYK and GAPDH and yeast PGK) and Sigma (rabbit muscle ENO and bovine heart LDH). Measuring PGAM activity in a Leishmania lysate The mutase ... of 3PGA to 2PGA was coupled to NADH oxidation by lactate dehydrogenase (LDH) via enolase (ENO) and pyruvate kinase (PYK), and following the concomitant decrease of absorbance...

Ngày tải lên: 16/03/2014, 16:20

... Taiwan 3 Department of Botany, Karnatak University, Dharwad, India 4 Tainan District of Agricultural Improvement and Extension Station, Council of Agriculture, Tainan, Taiwan Phytocystatins are ... with a Bio-Rad protein assay kit (Bio-Rad, Hercules, CA, USA) using BSA as a standard. In-gel antipapain assay Qualitative analysis of CeCPI protein was performed according to Michaud...

Ngày tải lên: 23/03/2014, 06:20

... 2006) doi:10.1111/j.1742-4658.2006.05311.x Lipopolysaccharide (LPS) and b-glucan from Francisella victoria, a fish pathogen and close relative of highly virulent mammal pathogen Francisella tularensis, have been analyzed using chemical and ... compo- nents: alanine, 3-aminobutyric acid (homoalanine or BABA), and a novel branched amino acid designated AA. HMBC correlations allow...

Ngày tải lên: 30/03/2014, 10:20

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... novel interactor of the C-ter- minus of Arabidopsis thaliana plasma membrane H + -ATPase (EC 3.6.3.6) (Morandini P, Valera M, Albumi C, Bonza MC, Giacometti S, Ravera G, Murgia I, Soave...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... PCR was performed in 50 lL containing 1 ng of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GA AAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) a...

Ngày tải lên: 19/02/2014, 07:20

... Aiba Y, Nakamura K, Namba T, Hirata M, Mazda O, Katsura Y & Narumiya S (1993) Thromboxane A2 receptor is highly expressed in mouse immature thymocytes and mediates DNA fragmentation and apoptosis. ... pGL3e: Prm3 AP)1 *, pGL3b: Prm 3a AP)1 *, pGL3e:Prm 3a AP)1 *, pGL3b:Prm3ab AP)1 *, pGL3e: Prm3ab AP)1 *, pGL3b: Prm3aa AP)1 *, pGL3e:Prm3aa AP)1 *, pGL3b:Prm3aaa AP)1 * and pGL3e:P...

Ngày tải lên: 19/02/2014, 16:20

... 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers encoded a Kozak consensus sequence as well as BamHI and EcoRI restriction sites. The PCR-amplified ... observed with M. brassicae CSPMbraA6 [32]. We observed also that BrC15-Ac was able to displace ASA, suggesting that brominated fatty acid and ASA both associated with W81 in the same l...

Ngày tải lên: 21/02/2014, 03:20