Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... C (1989) The limits of the DNase I-sensitive domain of the human apolipoproteinB gene coincide with the locations of chromosomalanchorage loops and deﬁne the 5¢ and 3¢ boundaries of the gene. ... Since then there has been an explo-sion of papers on the multitude of chromatin modiﬁca-tions and the factors that can either create orrecognize them. We now have a very detailed picture of the ... bp intervals. The lighter colour shadesdepict the bottom half of the nucleosome, and the exploded viewbelow the octamer depicts the arrangement of the histones con-tacting 73 bp of DNA within...

Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx

... N-terminal of the mature enzyme and by the ÔﬂapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionicinteractions established between Lys11/Tyr13 of ... duringprocessing and is absent from the mature form of the enzyme. Its functions are still unclear but a role in the vac-uolar targeting of the precursors has been proposed. The biological role of plant ... hybridization, in the digestive glands of the pitchers, the trapping organs of the plant [6]. Usingimmunohistochemistry and immunogold transmission EM,APs puriﬁed from the ﬂowers of the cardoon Cynaracardunculus...

Báo cáo khoa học: Structure and function of KH domains docx

... in which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja-cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... data from other KH domains to the KH1–KH2 domains of FMRP. The structure of the tandem KH1–KH2 domains of hFMRP provided the ﬁrst crystallographic description of the structural environment of the ... 6A–C). The ﬁrst position of the core recognition motif sits on top of the a1-helix, and then the phosphate backbone of the next two nucleo-tides interacts with the a1-helix and the GXXG motifon the...

Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identiﬁcation of two active site cysteines pptx

... that the subcellularlocalization of GlcNAc kinase in ﬁbroblasts is different from the localization of the enzymes of the de novo pathway [7], indicating a spatial separation of the twopathways, ... an antagonist of proteinphosphorylation [2]. Finally, it is the key substrate for the biosynthesis of sialic acids [3]. The function of the salvage pathway of UDP-GlcNAcbiosynthesis is not ... comparable to the hexokinase subunits [24]. Onelobe consists of the phosphate1 subdomain and the predicted a-helix I. The other lobe consists of the phos-phate2 and the adenosine subdomains, the predicted...

Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx

... estimation of the hydrodynamic radii of HAMLET (Rh= 26.9 A˚),which is intermediate of the hydrodynamic radii of the acidic molten globule state of a-lactalbumin(Rh= 20.9 A˚) and the theoretically ... ago and since then the HAMLETﬁeld has widened in scope, acquiring new members and enriching our understanding of the basic principlesunderlying protein self-assembly and acquisition of new functionality. ... fully understood. The active group of the matrix, the DEAE group, is posi-tively charged and might therefore bind negative mole-cules. At pH 8.5 (the pH of the conversion step) the fatty acid...

Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf

... compilation ª 2006 FEBS Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 Sad Halak1,*, Lari ... promoter [22]pETS2-X-II Expression of pcaH2G2 from Agrobacterium radiobacter S2 under the control of the T7 promoter[12]pMCS2-I-39B pcaG1 and pcaB 1S2 from A. radiobacter S2 inpBluescript ... Trp153,Lys282 and Arg315 are involved in catalysis. The alignment of the small CMLEs from different members of the Rhizobiales with these sequences demonstratedthat in A. radiobacter S2 (and the other...

Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... the SH3 domain of Noxo1 participates inregulation of Nox3, the role of the PX domain in Nox3activity remains unknown.In the process of cloning of human Noxo1, somespliced transcripts of the ... as the insert region is located between the pair of primers(Fig. 2A). The lengths of the PCR products of Noxo1b and Noxo1c were 225 and 240 bp, respectively. Todelineate the difference in the ... and expressed as the foldincrease relative to that of the band forNoxo1c in the absence of PMA.R. Takeya et al. Expression and function of Noxo1cFEBS Journal 273 (2006) 3663–3677 ª 2006 The...

Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

... at a scanning rate of 50 nm Æmin)1with a time constant of 2 s, step resolution of 0.1 nm, and bandwidth of 1 nm. The ﬁnal spectra weretaken from the average of ﬁve s cans. The VARSELECprogram ... excess of 3 for IFP–wt) and the shape o f Ix/x vs. x plot. Speciﬁcally, the initial slow rise of the l atter plot (from x ¼ 1) reﬂects that the self-association of the peptide is not tight ( on the ... and ^represent parallel and perpendicular polarized light, respectively. The bottom panel displays the result of deconvolution of the IR absorptionspectrum of the peptide f or analysis of the...

Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx

... or the availability of growth factors, hormones, and nutrients,result in the activation of signaling pathways that rap-idly and reversibly modulate the translation of RPmRNAs and the other ... documented thatthere are signaling pathways that regulate the transla-tional activity of RP mRNA for adjustment of the biosynthesis of ribosomes to the requirements of cellgrowth and differentiation. ... synthesis [9]. The identiﬁcation of the role of a speciﬁc RP is com-plicated by the high level of cooperativity among ribo-somal components and by the fact that the ribosome isessential for the cell....

Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens potx

... with the gene of the benzaldehyde lyase and for helpful discus-sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their helpwith data collection and ... to those of other ThDP-dependent enzymes [23]. In all enzymesThDP assumes a V-conformation resulting in a closeapproach between the C2 atom of the thiazolium ring and the N4¢ atom of the pyrimidine ... comparison of the relativeB-factors along the ThDP molecules shows that the Fig. 4. Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis. The secondary structure...

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