Báo cáo khoa học: Green fluorescent protein-tagging reduces the nucleocytoplasmic shuttling specifically of unphosphorylated STAT1 potx

... (2007) 815–826 ª 2007 The Authors Journal compilation ª 2007 FEBS Green fluorescent protein-tagging reduces the nucleocytoplasmic shuttling specifically of unphosphorylated STAT1 Thomas Meyer 1 , ... underestimate the actual shuttling rate of unphosphorylated native STAT1. The results confirm that the activation of STAT1 and hence its transcriptional ac...

Ngày tải lên: 30/03/2014, 09:20

... ATPase activity of the chaperone Hsp93. The motor activity of this AAA+ ATPase probably accounts for most of the ATP requirement of the import reaction, exerting the pulling force on the incoming ... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at t...

Ngày tải lên: 07/03/2014, 03:20

... by the energy of ATP hydrolysis, obviates the need of looping of intervening DNA. The in vivo data supporting the MutS–MutL foci formation suggests the possibility of extensive recruitment of ... [24,27,28]. Amplification of single mismatch by MutS–MutL The most important finding of the study relates to the massive aggregation of MutS in the presence of ATP w...

Ngày tải lên: 07/03/2014, 12:20

... iden- tified as the major and the minor forms of the holo- ACP structure, on the basis of their percentage contri- butions (65% and 35%, respectively) to the overall structure of the protein. The structures ... estimated on the basis of the linear regression of the fraction number of the molecular mass markers cytochrome c (CyC), carbonic anhydrase (CA), and B...

Ngày tải lên: 16/03/2014, 10:20

... angiogenesis [15]. Thus, the unusual versatility of aaRSs in higher eukaryotes is further increased by their interactions with nonsynthetase proteins. The association of the synthetases into multicompo- nent ... processes [1]. These noncanonical activities vary with the type of aaRS and its cellular location [2]. The expansion of synthetase function well beyond protein synth...

Ngày tải lên: 23/03/2014, 09:20

... example of the robustness of the protocol, the inter- action between DNA and the DNA-binding domain of the transcription factor MED-1 involved a substantial conformational change of the protein, ... steps of the cycle [63]. Importantly, motions of the cofactor-binding site are coupled with those of the substrate-binding site. So how are these data useful? Simil...

Ngày tải lên: 29/03/2014, 00:20

... In the presence of arabinose, the expres- sion of YidC is observed in cells harbouring the empty vector, whereas no YidC is detected in the absence of arabinose (Fig. 3A, lanes 1 and 2). In the ... between the precursor and mature forms. In the presence of YidC, precursor M13P2 is converted into its mature form (Fig. 4B, upper panel, lane 1). In the absence of YidC,...

Ngày tải lên: 30/03/2014, 03:20

... records Is There the Wikipedia Article about the Word in the Search Result? Is There Any Wikipedia Article in the Top 30 in the Search Result? Is There Any Snippet Left in the Search Result? Does the ... 2 shows the breakdown of the 500 unknown words in terms of their correct domains. The other 167 words belong to NODOMAIN. 5.2 Result of Blog Categorization Table 3 s...

Ngày tải lên: 31/03/2014, 00:20

... in the leaf node 1 . In the case of part -of- speech tagging, a classifi- cation will be made at each position (or time) of a word sequence, where the target feature is the syn- tactic class of the ... on the root, each branch represents a result of the test (which is labeled on the arc), and the correspond- ing leaf node contains the probabilistic distribution of...

Ngày tải lên: 31/03/2014, 03:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... that the enzy- matic activity of RPE65c is iron-dependent. Characterization of the kinetic parameters of the enzymatic activity of zebrafish RPE65c To determine the steady-s...

Ngày tải lên: 14/02/2014, 14:20