Báo cáo khoa học: Glycolipids with nonreducing end a-mannosyl residues that have the potential to activate invariant Va19 NKT cells pptx

... compilation ª 2007 FEBS Glycolipids with nonreducing end a-mannosyl residues that have the potential to activate invariant Va19 NKT cells Michio Shimamura1, Yi-Ying Huang1, Naoki Okamoto1, Yutaka ... Va19 NKT cells in the spleen. Thus, a-mannosyl glycolipids injected into micepossibly target Va19 NKT cells, given the lack ofimmune responses to the glycolipids in the culture of Va19 Tg cells ... enhanced responsiveness of Va19 Tg+ cells to the MR1 transfectants previously loaded with the a-mannosyl glycolipids suggests that Va19 NKT cells have the potential to recognize exogenous antigens...

Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx

... display of the PrPepitope on the PrP–VLP surface (Fig. 1D).Immunization with PrP–VLPs induces (auto-)Ab to the PrP peptide DWEDRYYRE The chosen murine prion epitope DWEDRYYRE issituated in the central ... prion-infected cells. ResultsPrP–L1 fusion protein self-assembles intovirus-like particles (PrP–VLPs) that express the PrP-DWEDRYYRE epitope To generate chimeric VLPs that display a PrP epitopeon the ... pheochromocytoma cells Recent studies have found an association between the ability of Abs to recognize native PrPCexpressed on the cell surface and their ability to cure prion-infected cells in...

Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf

... transformation. These results further support the idea that the ability of the GST isozymes to inducetransformation is dependent on their ability to conjugate4-HNE to GSH. Furthermore, these results ... after the injection, any cells killed by the injection procedure were lifted off the dish leaving only the injected cells that survived the injection. Such cells wereﬂat and attached to the dish ... number of cells underwent apoptosis aftermicroinjection. The apoptotic cells could be identiﬁed, asthey showed activation of caspase-3 detected by staining the cells with antibodies to cleaved...

Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... dose-dependentlytranslocated ﬁrst to plasma membranes and then to endosomes, in which it associates with phosphorylatedIR. These results suggest that Grb14 is recruited by the activated IR at the ... lead to interesting newinformation on the role of this adaptor protein. The endosomal colocalization and ⁄ or cointernaliza-tion of the activated IR and Grb14 raises the questionas to whether ... fractions. Altogether, these data support a model wherebyinsulin stimulates the recruitment of endogenous Grb14 to the activated IRat the plasma membrane, and induces internalization of the Grb14–IR...

Báo cáo khoa học: 7,8-Diaminoperlargonic acid aminotransferase from Mycobacterium tuberculosis, a potential therapeutic target Characterization and inhibition studies pptx

... °C. The residual activity of the enzyme solution was then measured, taking into account the dilution factor due to the dialysis step. A blank contain-ing all components but without the inhibitor ... sufﬁcient to reverse the growth inhibi-tion. Furthermore, adding 100 lm DAPA to the med-ium in the presence of avidin also reversed the growthinhibition. Taken together, these data indicate that, ﬁrstly, ... Kmvaluesfor the latter are 3–4 times higher, and that the kcatfor the M. tuberculosis enzyme is eight times lower than that of the E. coli enzyme. Furthermore, the inhibitionconstant, KiKAPA, for the...

Báo cáo khoa học: Identiﬁcation of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt

... prT1. prT1 was linearized with BamHI,andthendigestedwithBal31 nuclease. The Bal31 digest wasblunt-ended and digested with NotI. The resulting digestwas ligated into the EcoRV and NotIsitesofpcDNA4,obtaining ... enzyme. The aﬃnity for the acceptor apomucin, on the other hand, was essentiallyunaﬀected. The functional importance of the introducedserine residues was further demonstrated by the inhibition ofall ... residues interacting with the UDP moiety of the sugar donor. For the functional analysis of the cysteine residues, several conserved cysteine residues in GalNAc-T1were mutated individually to...

Báo cáo khoa học: Lack of stabilized microtubules as a result of the absence of major maps in CAD cells does not preclude neurite formation pot

... beelicited [33]. The rapid retraction of neurites after the addition ofserum led us to consider the possibility that the cyto-skeleton of CAD cells should have peculiar properties.Thus, in the present ... corresponding to each MAP and the absenceof their mRNAs (with the exception of that of Tau)(Fig. 5). mRNA corresponding to Tau was detected inCAD cells in amounts similar to that in brain ... stable-tubule-only-peptide(STOP) [6–9]. There are also other MAPs that have been studied to a lesser extent (spectraplakins, adeno-matous polyposis coli, doublecortin, dishevelled), aswell as other proteins binding to...

Báo cáo khoa học: b3-Adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat pptx

... h)before the beginning of each experiment. They were thendivided into three groups, one maintained at 24 °Candtheother two exposed to cold (6 °C) for either two or 10 days. The fat pads along the ... 129Sv/ev)[23], was then backcrossed to obtain the mutation on the C57BL/6J genetic background. In the present work, westudied the effect of b3-adrenoceptor-deﬁciency on the Correspondence to M. Jimenez, ... b3-adrenoceptor might notbe the only b-subtype controlling the emergence of brownadipocytes in WAT.Another open question is the origin and the real nature of the multilocular cells expressing UCP1 that...

Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different ﬂanking bases preference pptx

... the different AtERFs.On the other hand, some other residues reported to determine the geometry of the a-helix relative to the b-sheet were not as conserved as these other residues, but instead ... CBF1. The secondarystructure scheme is indicated above the sequence. The conservedamino acid residues that directly contact with DNA bases and the other conserved amino acid residues that do ... by the CaMV 35S promoter, together with the luciferasereporter gene containing four tandem copies of either the DRE motif or its mutants at the upstream regula-tory region, was coexpressed into...

Tài liệu Báo cáo khoa học: "Learning with Unlabeled Data for Text Categorization Using Bootstrapping and Feature Projection Techniques" doc

... et al., 1991). The window is a slide from the first word of the document to the last in the size of the window (60 words) and the interval of each window (30 words). Therefore, the final output ... Since the keywords for text categorization must have the power to discriminate categories as well as similarity with the title words, we assign a word to the keyword list of a category with the ... a vector indicating the affinity of each word to it. 3) Similarity formulae The similarity of W1 to W2 is the average affinity of the contexts that include W1 to W2, and the similarity...

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