Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung ﬁbroblasts is speciﬁcally mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot

... mechanisms forCCN2 ⁄ CTGF persistence in ﬁbrotic gingiva: interac-tions between cAMP and MAPK signaling pathways, and prostaglandin E2- EP3 receptor mediated activation of the c-JUN N-terminal kinase. ... 2008 The Authors Journal compilation ª 2008 FEBS 3679 Downregulation of protease-activated receptor- 1 in human lung ﬁbroblasts is speciﬁcally mediated by the prostaglandin E2 receptor EP2 through ... Sokolova E, Grishina Z, Bu¨hling F, Welte T & ReiserG (2005) Protease-activated receptor- 1 in human lung ﬁbroblasts mediates a negative feedback downregulation via prostaglandin E2. Am J...

Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

... B and L involved in cancer growth and metastasis [2]. Since the involve-ment of cathepsin B in cancer metastasis was originallydescribed by Sloane et al. [3], cathepsins, and especiallycathepsin ... (repre-senting intracellular cystatin C) was quantiﬁed by ELISA and the cystatin C level was correlated to the protein content of the celllysate. The lines are drawn through the average value of the ... heat denaturation of the endogenous cysteine proteinases, the cysteine protease inhibitory capacity of the cell lysate wasdetermined by measuring the inhibition of (A) papain and (B)cathepsin...

Báo cáo khoa học: A chloroplast RNA binding protein from stromal thylakoid membranes speciﬁcally binds to the 5¢ untranslated region of the psbA mRNA potx

... mRNA corresponding to positions)147 to +24 relative to the AUG): T7-psbB5¢(5¢-GTAATACGACTCACTATAGGGTAAATTAATTTAATTTAAAATC-3¢)andpsbB3¢ (5¢-TACACGATACCAAGGTAAACC-3¢). Each template contained ... psbA mRNA corresponding to positions )91 to +13relative to the AUG); T7-psbA5¢ (5¢-GTAATACGACTCACTATAGGGTACCATGCTTTTAATAGAAG-3¢ )and 2054 (5¢-GATCCATGGTCATATGTTAATTTTTTTAAAG-3¢); )36-RNA (wild-type ... coding region of the C-terminalamino acids of D1 and the 3¢ UTR of the psbA mRNA) wasampliﬁed with the oligonucleotides psbA3/1 (5¢-CTCTAGCTCAAACAACT-3¢)andpsbA3/2 (5¢-GCCTATGGTAGCTATTA)3¢)...

Báo cáo khoa học: Characterization of sequence variations in human histone H1.2 and H1.4 subtypes pptx

... cell lines and tissues investigated containedonly alanine at this location.To conﬁrm the H1.2 Ala17Val sequence variation, a 183 bp PCR product was ampliﬁed in the 5¢-UTR, and the start of the ... had a speciﬁc role in DNAdamage-induced apoptosis [14]. It is useful therefore toexamine the properties and expression of these variantsas this furthers a better understanding of the relevance of ... lym-phoma. The signiﬁcance of these sequence variants is unclear, but increas-ing evidence indicates that minor sequence variations in linker histonesmay change their binding characteristics, in uence...

Báo cáo khoa học: K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase pdf

... panel). The nuclear compartment was stained usingDAPI, and the cytoplasm was stained using rhodamine phalloidin. Images entitled ‘Merged’ were created by overlaying images obtained by staining with ... DNA and positively chargede-amino groups (lysine residues) and guanidino groups(arginine residues) in histones.Histones consist of a globular C-terminal domain and a ﬂexible N-terminal tail ... notshown).Biotinylation of histone H3 depended on biotinidase and holocarboxylase synthetase. For example, biotiny-lation of K18 in histone H3 was less abundant in ﬁbroblasts from biotinidase- and holocarboxylasesynthetase-deﬁcient...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... against hepatic drug-metabolizing dysfunction by modulation of the in ammatory response.AbbreviationsALT, alanine aminotrasferase; AST, aspartate aminotrasferase; CLP, cecal ligation and puncture; ... KCs[25]. The expression and activity of CYP2E1 weredownregulated in a rat hepatoma cell line after the administration of proinﬂammatory cytokines, leadingto a loss of catalytic activity. This downregulation ... a DNA-binding nuclear protein, is released actively by monocytes ⁄ macrophages and pas-sively by cell death, and plays a critical role in the mediation of immune responses in several in amma-tory...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... Horse-radish peroxidase-conjugated antibody against caspase-3(#610325) was from BD Pharmigen, and antibody againstb-actin (A5 441) was from Sigma.Statistical analysisAll data are expressed as the ... unsaturated FFAs into the cells. After theirinternalization, FFAs are converted to fatty acyl-CoA, a reaction catalyzed by ACS. Fatty acyl-CoAs are acti-vated forms of fatty acids that can ... notedthat TrC was not able to inhibit thapsigargin-inducedCtrl3 h12 h24 h 36 h6 hOAOASA/OASA/OASA/OASA/OASA/OASAOAFL1-LogCountsOAOASASASASACtrlCtrlCtrlCtrlCtrlSA OA SA/OA A BFig....

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... 5¢-GTAGGCATGAGAACGGGAAG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGAAA-3¢ and for CERK-negative forward 5¢-CCGCAAGAGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGACACGGAGAT-3¢, as a negative control PCR primer. The condition ... which encodes a region of the protein that is known to be essential for kinase activity and contains a diacylglycerol kinase- likecatalytic domain [2]. As shown in Fig. 5A, cell death and limited ... survival in mammalian cells [4]. Arabidopsis, carrying a CerKmutation, exhibits a spontaneous cell-death pheno-type and accumulates Cer late in development [4]. In A5 49 human lung adenocarcinoma...

Báo cáo khoa học: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus pptx

... (AAA-1), a middle domain, and a secondAAA+ module (AAA-2). Each AAA+ module contains highly conservedWalkerA and WalkerB motifs, and two arginines (AAA-1) or one arginine(AAA-2). Here, we investigated ... arginine of AAA-1, R33 2A, decreased the chaperone activity and desta-bilized the hexamer, and that the alanine mutation of the P1 arginine of AAA-2, R75 6A, decreased the AT-Pase and chaperone activities ... arginines in TClpB. By combining an arginine mutation with the WalkerAmutation in the other AAA+ module, we tested the roles of the arginines in ATP binding and hydrolysis in each module. The arginine...

Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc

... activationmechanism and conﬁrm the prominent involvement of lysine residues in interactions between extracellular matrix proteins and in ammatory cells.AbbreviationsAHA, 6-amino-hexanoic acid; ... Boonmark N, Skocir P, Lawn R &Plow EF (1996) A quantitative immunoassay for the lysine-binding function of lipoprotein (a) . Application torecombinant apo (a) and lipoprotein (a) in plasma.Arterioscler ... activate PMNs [7]. Carbamylation is the post-translational modiﬁcation of proteins caused by the non-enzymatic binding of isocyanic acid, a reactiveurea by- product, to e-amino-groups of lysine...

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