Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... synthase (NOS) and heat
shock protein 90 (HSP90) by calpain is modulated by the
formation of a NOS–HSP90 heterocomplex
Monica Averna, Roberto Stifanese, Roberta De Tullio, Franca Salamino, Sandro ... approximately 80 90% of eNOS protein and
A
B
Fig. 2. Levels of calpain isoforms and calpain substrates in the aorta
of NMS and HM...
... produced, isolated and analysed novel catalase
variants in which the iron atom of the haem prosthetic group is replaced
by other metals, i.e. cobalt, gallium, tin, and zinc, and also variants con-
taining ... case of Ga-PP-KatA, an additional protein
band of about 110 kDa was observed. This band cor-
responds to KatA dimer, as determined by immuno-
blot analysis (Fig...
... Medicina y Farmacia., Universidad de Valencia, Valencia, Spain
Several clinical trials have revealed that individuals who were
given b)carotene and vitamin A did not have a reduced risk
of cancer ... in
controlling the expression of p53 and related genes that are
essential for maintaining the integrity of tissues.
Materials and methods
Rats
Wistar rats (Charles River Lab...
... first
demonstration of an in vivo activation of transglutaminase
leading to the formation of a cross-link in intracellular
proteins.
Keywords: starfish; sperm; transglutaminase; chromatin;
acrosome reaction.
The ... the acrosome reaction
Kazuto Nunomura*, Satoru Kawakami†, Takahiko Shimizu†, Tomohiro Hara, Kazuhiro Nakamura,
Yudai Terakawa, Akiko Yamasaki and Susumu Ikeg...
... containing 1) [9]. The KIND domain in these
proteins is localized to the N-terminal region, and their
specific functional domains are located in the C-termi-
nal region. The C-lobe of protein kinases ... the protein protein interaction
between v-KIND and MAP2 (i.e. the MAP2-binding
core module in v-KIND and the v-KIND-binding core
module in MAP2) across a...
... sampled. Their gonads were
immediately dissected: a large transverse section of all the
gonadic area was taken for histological examination, and
the rest of the gonad was placed in total RNA and ... (lane 7). Twelve
micrograms of total protein extract from each tissue was loaded
into the gel. A single band of about 79 kDa was detected in female
and male gonads....
... peptide. This signal sequence is involved in
guiding the protein into the targeting and translocating
pathway by interacting with the membrane and other
components of the cellular secretory machinery ... the membrane
were excised and the N-terminal amino acid sequence
of each protein was determined by automated Edman
degradation.
Cleavage of prestreptokin...
... positive
mode. The spray voltage was set to 4500 V, the capillary
temperature was 375 °C and sheath and auxiliary gas was
optimal at 60 and 5 arbitrary units, respectively. Skimmer
offset was at 10 V and ... type 1 and 2 [6,8]. It
has been shown that patients with asthma and airway
in ammation have increased expression of 15-LO-1
protein and increased activity of 15-L...
... in
these parameters. Analysis of the changes in internal
hydrogen bond composition for the protein- glycan con-
jugates indicates that for all of the conjugates there was
also an increase in these ... of the protein decreases at
increasing glycosylation levels. This is in accord with
the increased thermodynamic stability exhibited by
natural glycoproteins [34,56...
... by X-ray crystal-
lography in these structures is a string of water mole-
cules between the a- helix and the major b-sheet that
are distinctly located in the wild-type protein and in
the variants. ... or
nonself proteins are usually 8–12 amino acids long and
are accommodated in a binding groove of the molecule
by means of HLA allele-characteristic ‘a...