Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot

... synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex Monica Averna, Roberto Stifanese, Roberta De Tullio, Franca Salamino, Sandro ... approximately 80 90% of eNOS protein and A B Fig. 2. Levels of calpain isoforms and calpain substrates in the aorta of NMS and HM...

Ngày tải lên: 30/03/2014, 04:20

... produced, isolated and analysed novel catalase variants in which the iron atom of the haem prosthetic group is replaced by other metals, i.e. cobalt, gallium, tin, and zinc, and also variants con- taining ... case of Ga-PP-KatA, an additional protein band of about 110 kDa was observed. This band cor- responds to KatA dimer, as determined by immuno- blot analysis (Fig...

Ngày tải lên: 06/03/2014, 11:20

... Medicina y Farmacia., Universidad de Valencia, Valencia, Spain Several clinical trials have revealed that individuals who were given b)carotene and vitamin A did not have a reduced risk of cancer ... in controlling the expression of p53 and related genes that are essential for maintaining the integrity of tissues. Materials and methods Rats Wistar rats (Charles River Lab...

Ngày tải lên: 08/03/2014, 08:20

... first demonstration of an in vivo activation of transglutaminase leading to the formation of a cross-link in intracellular proteins. Keywords: starfish; sperm; transglutaminase; chromatin; acrosome reaction. The ... the acrosome reaction Kazuto Nunomura*, Satoru Kawakami†, Takahiko Shimizu†, Tomohiro Hara, Kazuhiro Nakamura, Yudai Terakawa, Akiko Yamasaki and Susumu Ikeg...

Ngày tải lên: 31/03/2014, 07:20

... containing 1) [9]. The KIND domain in these proteins is localized to the N-terminal region, and their specific functional domains are located in the C-termi- nal region. The C-lobe of protein kinases ... the protein protein interaction between v-KIND and MAP2 (i.e. the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across a...

Ngày tải lên: 14/02/2014, 19:20

... sampled. Their gonads were immediately dissected: a large transverse section of all the gonadic area was taken for histological examination, and the rest of the gonad was placed in total RNA and ... (lane 7). Twelve micrograms of total protein extract from each tissue was loaded into the gel. A single band of about 79 kDa was detected in female and male gonads....

Ngày tải lên: 07/03/2014, 00:20

... peptide. This signal sequence is involved in guiding the protein into the targeting and translocating pathway by interacting with the membrane and other components of the cellular secretory machinery ... the membrane were excised and the N-terminal amino acid sequence of each protein was determined by automated Edman degradation. Cleavage of prestreptokin...

Ngày tải lên: 23/03/2014, 21:21

... positive mode. The spray voltage was set to 4500 V, the capillary temperature was 375 °C and sheath and auxiliary gas was optimal at 60 and 5 arbitrary units, respectively. Skimmer offset was at 10 V and ... type 1 and 2 [6,8]. It has been shown that patients with asthma and airway in ammation have increased expression of 15-LO-1 protein and increased activity of 15-L...

Ngày tải lên: 30/03/2014, 04:20

... in these parameters. Analysis of the changes in internal hydrogen bond composition for the protein- glycan con- jugates indicates that for all of the conjugates there was also an increase in these ... of the protein decreases at increasing glycosylation levels. This is in accord with the increased thermodynamic stability exhibited by natural glycoproteins [34,56...

Ngày tải lên: 19/02/2014, 05:20

... by X-ray crystal- lography in these structures is a string of water mole- cules between the a- helix and the major b-sheet that are distinctly located in the wild-type protein and in the variants. ... or nonself proteins are usually 8–12 amino acids long and are accommodated in a binding groove of the molecule by means of HLA allele-characteristic ‘a...

Ngày tải lên: 06/03/2014, 00:21