Báo cáo khoa học: Identification and functional characterization of an aggregation domain in long myosin light chain kinase ppt

... Tumor necrosis factor-induced long myosin light chain kinase transcription is regulated by differentiation-dependent signaling events: characterization of the human long myosin light chain kinase promoter. ... N-terminal extension of the isoform of smooth muscle long myosin light chain kinase. Cell Res 16, 367–376. Aggregation domain in myosin lig...

Ngày tải lên: 30/03/2014, 04:20

... Takashi Nishino 3 and Kei Kamino 1 1 Marine Biotechnology Institute, Iwate, Japan 2 Pharma Design, Inc., Tokyo, Japan 3 Department of Chemical Science & Engineering, Faculty of Engineering, Kobe ... protein is a cement compo- nent and not a component involved in calcification. Western blot analysis of the primary cement, and of the protein fractions in the calcareous base...

Ngày tải lên: 16/03/2014, 05:20

... SP1 and TBP binding sites found upstream exon B. This finding would argue for a ubiquitous and low-level expression of exon B containing transcripts. On the other hand, the presence of CREB and ... (1988) Asparagine-linked oligo- saccharides on lutropin, follitropin, and thyrotropin. I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, an...

Ngày tải lên: 08/03/2014, 08:20

... c.secombes@abdn.ac.uk Abbreviations: IL, interleukin; IL-1Ra, interleukin 1 receptor antagonist; ICE: interleukin 1b converting enzyme; IL)18 BP, interleukin 18 binding protein; IFN, interferon; c-IP10, interferon gamma induced ... Genomic DNA and the deduced protein sequence of rainbow trout IL-18. Exons and introns are indicated in uppercase and lower- case, respectively. Intron s...

Ngày tải lên: 16/03/2014, 16:20

... report concerning an enzyme involved in the kynurenine pathway in A. gambiae. Our analysis on 3-HKT contributes to a better understand- ing of the XA metabolism in the mosquito and offers a new ... synthesis of the transamination product KA was monitored at 330 nm and quantified by interpolating the experimental data in the corresponding standard curve obtained as above. The...

Ngày tải lên: 16/03/2014, 23:20

... pattern and degree of extension from each Hep residue varies between and within strains. The presence of nonglycose residues, including phosphate- containing substituents and ester linked acetyl ... again perhaps indicating that steric interference of the two chains precludes coincident expres- sion of both. It is noteworthy that the majority of Sial- lNnT-containing glycoform...

Ngày tải lên: 23/03/2014, 21:21

... volume and energetic properties of the binding of CO to hemoproteins. Biophys J 66, 89–98. 42 Tuckey RC & Kamin H (1983) Kinetics of O 2 and CO Binding to adrenal cytochrome P-450scc. Effect of ... plasmids: differ- ential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of A. Zo ¨ llner et al. Functional characteriza...

Ngày tải lên: 30/03/2014, 04:20

... single band of predicted size. The DNA band was digested with NdeIandXhoI, and cloned into the NdeIandXhoI sites of pET22b. The H151A mutant protein of SMC expressed in E. coli was purified using the ... activity of recombinant SMC. As shown in Fig. 4, the mutation markedly reduced the maximum rate of hydrolysis of sphingomyelin, and the affinity of sphingo- myelin for the...

Ngày tải lên: 30/03/2014, 13:20

... superimposed structures of conomarphin (red) and L-Phe13-conomarphin (gray). The side chain of L-Phe13 might cause spatial hindrance to the side chain of Lys9 and Hyp10 in forming the tight loop as in conomarphin. ... side chain of Lys9, Hyp10 and D-Phe13 of cono- marphin is shown in stick mode, and the side chain of L-Phe13 in L-Phe13-conomar- phin is show...

Ngày tải lên: 18/02/2014, 17:20

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... self-standing structure protruding from the interface between the DNA binding and the active site subdomains, we man- aged to model the entire domain without it. Another signific...

Ngày tải lên: 18/02/2014, 18:20