Báo cáo khoa học: Identification in the yeast Pichia stipitis of the first L-rhamnose-1-dehydrogenase gene docx
Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... implicated in retraction of the PilA4-comprising DNA translocator transporting the DNA through the periplasmic space. Binding of the DNA to the DNA-binding protein ComEA on the surface of the inner ... Organization of pilMNOWQ and generation of gene frag- ments fused to the gene for maltose-binding protein (malE). The arrows indicate the directions of tra...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification in the yeast Pichia stipitis of the first L-rhamnose-1-dehydrogenase gene docx
... either at the N-terminus or at the C-terminus. To introduce the histidine-tag at the N-terminus, we introduced a coding sequence for MHHHHHHGG before the original start codon. To introduce the ... compar- ing the results of the SDS ⁄ PAGE that was done after the zymogram staining with those of the SDS ⁄ PAGE of the active fractions after the DEAE column and corre...
Ngày tải lên: 30/03/2014, 04:20
Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... insulin receptor-interacting region (PIR) [6] and inhibits the tyrosine kinase activity of the IR [7]. On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain ... inhibitor in the substrate- binding groove of the kinase, whereas the SH2 domain interacts with phosphorylated tyrosine residues of the...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3. In addition, for stat6 and foxp3, there were ... adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain, which is implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 doma...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the buffer. Therefore, a fixed aliquot of the protein was incubated with the indicated ... basic proteins it might be that either methylation of the lysine or specific substitutions in the amino acid sequence, increasing the number of basic residues of lysi...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx
... investigate the possible existence of different isoforms of the PKA C-subunit in the sea mussel M. galloprovincialis. Results Separation of different isoforms of the C-subunit In order to demonstrate the ... are not generated as a consequence of the PTMs typical of the PKA C-sub- unit, but rather they differ in their amino acid sequences. In most mammalian sp...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... and SmRXR1(Glu251-Asn376) in vitro. DNA binding of a protein containing 20 amino acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the DBD of SmNR1 (Ile247-Ser372) ... in most NRs, and the length of the hinge in SmNR1 thus can be estimated to be 140 amino acids. The role of the large hinge in schistosome NRs remains unknow...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Identification of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot
... attached to the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transiently or stably, with lamins, chromatin proteins and a vari- ety of regulatory proteins, including ... emerin. Emerin is the product of a gene linked to EDMD [19,21]. An integral membrane protein specifically loca- ted at the inner nuclear membrane, emerin, like other INM p...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... clarified in detail and could play a key role in the detoxification processes. In this study we examined the role of Bcp2 in order to increase the knowledge of the enzymatic activity involved in the ... periods of time (Fig. 3). As shown, the addition of stressors to the cul- tures inhibits growth without killing the cells. The hybridizing band in the nor...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
... catalytic site of GalNAc-T1, might be involved in the catalytic function of the enzyme. To investigate whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we ... a major site of interaction with UDP-GalNAc, Cys214 is also involved in binding. PCMPS and DFP modification of the serine-mutants of GalNAc-T1 We also examined the se...
Ngày tải lên: 08/03/2014, 10:20