Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx

... for gene therapy in cancer. Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the ... of the Pokemon gene. Computer analysis of putative transcription factor-binding sites For a rough understanding of the regulation of the Pok...

Ngày tải lên: 30/03/2014, 04:20

... for the Pex1p–Pex6p interaction and their functional role in peroxisome biogenesis. Results The contribution of Pex1p and Pex6p to peroxisomal biogenesis is well established on the basis of the ... mislocalization of peroxisomal matrix proteins to the cytosol. Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional...

Ngày tải lên: 07/03/2014, 16:20

... in quenching of protein Trp fluorescence and in an increase of the specific DAUDA emission after the noncovalent incorporation of the ligand. Binding of DAUDA to Ag-NPA-1 caused  20% quenching of the emission ... 7B). These observations suggest that Ag-NPA-1 is localized mainly in cells of the inner hypodermis and the epithe- lium of the oviduct as well as extra...

Ngày tải lên: 16/03/2014, 18:20

... where the coding regions of the putative d-AAT and BCAT were sub- cloned into pRSET-B, the recombinant proteins were nearly all recovered in the insoluble fraction. Therefore, the coding regions of ... especially in the mammalian forebrain, throughout the lifespan of the animal. This amino acid binds to the Gly-binding site of the N-methyl-d-aspartate subtype of...

Ngày tải lên: 30/03/2014, 04:20

... USP7/ HAUSP, and the length of the polyQ region influences the strength of the interaction [163]. Unfortunately, the central part of the Josephin domain is difficult to model because of low sequence ... the stability of t he dimer. The first c luster includes F70/V336 and I72/Q338 ( both in b5 s trand) of D 3 and F 27/Y289 (b2 strand), L67/M324, V70/I327 and...

Ngày tải lên: 30/03/2014, 15:20

... 5). Because of the relative insensitivity of front-end desaturases and elongases to the presence of an x3-double bond and of x3-desaturases to the structure of the carboxyl end of the fatty acids, the ... the synthesis of EPA in P. tricornutum and that the preferred route relied on intermediates of both the x6- and the x3-pathway (see Fig. 1). The...

Ngày tải lên: 31/03/2014, 09:20

... interaction between strands b2 and b6 of neighboring monomers. The p26 modifications examined in this study are not located in either of these strands and they are not con- sidered further. As the basic p26 ... protein regions were excluded. Application of procheck [76] revealed that the stereochemical quality of the model was reliable, with 84% of the residues in the...

Ngày tải lên: 07/03/2014, 12:20

... Lophochotrozoans, the third major group of bilaterian animals, have remained in the background of the molecular survey of metazoan development. We report the cloning and functional study of the central part of ... report the cloning and functional study of the central part of the BMP pathway (the Cg-BMPR1 type I receptor and Cg-TGFbsfR2 type II rec...

Ngày tải lên: 07/03/2014, 21:20

... hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remains unclear and it is uncertain whether it has the capacity to ... interacts with both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydrox...

Ngày tải lên: 15/02/2014, 01:20

... the majority of the residues. There were no disordered regions in the map. The quality of refinement of the two crystal forms is shown in Table 1. Cis peptides were found between R81 and P82 and ... attempt was made to understand the molecular basis of the lower thermal stability. Results and Discussion Preliminary characterization and crystallization of the p...

Ngày tải lên: 18/02/2014, 14:20