Báo cáo khoa học: Identification of critical residues of subunit H in its interaction with subunit E of the A-ATP synthase from Methanocaldococcus jannaschii potx

... synthase. One B subunit has been removed from the A 1 section to reveal the D subunit within the A 3 B 3 hexamer. The A subunit is attached to the N-terminal domain (E N ) of subunit E by the ... min for each step of the experiment. The change of chemical shift was monitored in the HSQC spectra. The same procedure was followed for the binding experi...

Ngày tải lên: 30/03/2014, 04:20

... acceptor in the formation of the tetrahedral peptide intermediate. The difference in chloride sensitivity between ACE2 and ACE was investigated, and the absence of a second chloride-binding site ... This effect would only be present in ACE as the site is absent from ACE2. Interestingly, the R514Q mutant at the CL2 site of ACE2 has limited effect on the activity...

Ngày tải lên: 20/02/2014, 01:20

... determine whether RGG3 of EWS modulates the formation or unwinding of Htelo G-quadruplex DNA. To determine whether the RGG3 binding affected the stability of the G-quadruplex structure of Htelo, we ... block the interaction between the EAD and RNA polymer- ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelo bind...

Ngày tải lên: 15/02/2014, 01:20

... lgÆmL )1 streptomy- cin (Invitrogen) at 28 °C. In each well within a plate, either five spleens or five head kidneys were used. The three plates containing the spleens or the head kidneys contained three wells ... as the region in which this gene was found in the zebrafish genome shared no synteny with the human genome. The zebrafish gen- ome was searched using the human s...

Ngày tải lên: 16/02/2014, 09:20

... deter- mine the gene sequences, the putative promoter sequences were predicted by a neural network-based program [41], and the ORFs of these two genes were determined with the Glimmer method ... parental strain after an aerosol chal- lenge with viable bacteria. The difference between these two mutants in bacterial clearance might reflect differences in the integrity of...

Ngày tải lên: 18/02/2014, 14:20

... loca- tion of the intron within the MH1-encoding region is conserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1B is shared by human Smad3 [16]. The location ... help to explain these differences in binding preference. Although a modest decrease in the interaction strength between SmSmad1B and SmSmad4 was observed in th...

Ngày tải lên: 18/02/2014, 16:20

... from the branchial tissue that is different from the one previously sequenced. In the present study, we show that the two CA sequences are differentially expressed in tissues of the worm. These ... Positive staining with the antisense RpCAtr probe. (L) Higher magnification of the lobule showing the intensity of the labeling throughout the bacteriocytes. (M)...

Ngày tải lên: 18/02/2014, 16:20

... differ- ent time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle, showing that gills are more sensitive to thermal ... responsive than mantle. We hypothesize that the observed patterns reflect func- tional differences between these two tissues. A number of genes that were highly expressed in gills...

Ngày tải lên: 18/02/2014, 16:20

... in the region encoding the N- terminal portion of the protein (including the signal peptide and small tandem repeats), a large exon com- prises the middle region of the ORF, which encodes the ... morphologies have been discussed. These isolated single proteins have some activity in changing crystal morphologies; how- ever, analyses of the single proteins has not led...

Ngày tải lên: 18/02/2014, 17:20

... a detailed understanding of the biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes. The biosynthesis ... olean-3b,24-di- acetoxy-12-ene, except for the presence of several back- ground peaks (the amount of authentic sample was adjusted to equalize the height of both peaks...

Ngày tải lên: 19/02/2014, 07:20