Báo cáo khoa học: Identification of critical residues of subunit H in its interaction with subunit E of the A-ATP synthase from Methanocaldococcus jannaschii potx
Báo cáo khoa học: Identification of critical residues of subunit H in its interaction with subunit E of the A-ATP synthase from Methanocaldococcus jannaschii potx
... synthase. One B subunit has been removed from the A1section to reveal the D subunit within the A3B3hexamer. The A subunit is attached to the N-terminal domain (E N) of subunit E by the ... min for each step of the experiment. The change of chemical shift was monitored in the HSQC spectra. The same procedure was followed for the binding experiments with 15N-labelled H1 –47 and the ... support the viewthat the a-helical subunit H forms one of the twoperipheral stalks of the enzyme, with its C-terminusconnected to the N-terminal part of the catalyticA subunit and its N-terminus...
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Tài liệu Báo cáo khoa học: Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis docx
... acceptor in the formation of the tetrahedral peptide intermediate. The difference in chloride sensitivity between ACE2 and ACE was investigated, and the absence of a second chloride-binding site ... Thiseffect would only be present in ACE as the site isabsent from ACE2. Interestingly, the R514Q mutantat the CL2 site of ACE2 has limited effect on the activity of ACE2 in either the presence ... surface of endothelial cells, the renal tubularepithelium and also the epithelia of the lung and the small intestine [1–3]. Here ACE2 is poised to meta-bolize circulating peptides which may include...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... determinewhether RGG3 of EWS modulates the formation orunwinding of Htelo G-quadruplex DNA. To determinewhether the RGG3 binding affected the stability of the G-quadruplex structure of Htelo, we ... block the interaction between the EAD and RNA polymer-ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelobinding of RGG3.To gain further insight ... specificity. Here we show that the Arg-Gly-Gly (RGG) domain of the C-terminal in EWS binds to the G-richsingle-stranded DNA and RNA fold in the G-quadruplex structure.Furthermore, inhibition of...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... lgÆmL)1streptomy-cin (Invitrogen) at 28 °C. In each well within a plate, eitherfive spleens or five head kidneys were used. The three platescontaining the spleens or the head kidneys contained threewells ... as the region in whichthis gene was found in the zebrafish genome shared nosynteny with the human genome. The zebrafish gen-ome was searched using the human stat6 amino acidsequence directly ... and human sequences appearto be quite diverse. Together with the well-conservedgene organization, there is a conservation of syntenybetween the human and zebrafish genomes where the t-bet and...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... deter-mine the gene sequences, the putative promoter sequenceswere predicted by a neural network-based program [41],and the ORFs of these two genes were determined with the Glimmer method ... parental strain after an aerosol chal-lenge with viable bacteria. The difference betweenthese two mutants in bacterial clearance might reflectdifferences in the integrity of their outer membrane,binding ... detected in that of O3 5E (Fig. 2B,D, lane 1), showing a single insertion into the genome of each mutant.To determine whether the insertion had a polareffect on the upstream or downstream gene,...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... loca-tion of the intron within the MH1-encoding region isconserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1Bis shared by human Smad3 [16]. The location ... help toexplain these differences in binding preference.Although a modest decrease in the interaction strengthbetween SmSmad1B and SmSmad4 was observed in the presence of the wild-type SmTbRI ... tobe expressed in all stages of development, with the high-est level in cercariae. As the cercariae are the free-livingand infective stage of schistosomes, it is possible that the upregulation...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
... from the branchial tissue that is different from the one previously sequenced. In the present study, weshow that the two CA sequences are differentiallyexpressed in tissues of the worm. These ... Positive staining with the antisense RpCAtr probe. (L) Higher magnification of the lobuleshowing the intensity of the labeling throughout the bacteriocytes. (M) Negative control with the sense RpCAtr ... cytoplasm of the cells. The stainingappears to be specific of the probe sequence because the staining is clear with the complementary sequenceto RpCAbr, but not with the sense probe (negativecontrol;...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
... differ-ent time points over the course of the thermal stress. Differential geneexpression levels were much higher in gills than in the mantle, showing thatgills are more sensitive to thermal ... responsive than mantle. Wehypothesize that the observed patterns reflect func-tional differences between these two tissues. A number of genes that were highly expressed in gills showeda biphasic ... folding of cytoskeletal proteins that are actively synthesizedand ⁄ or renatured under these conditions. The upregu-lation of all of these chaperones confirms the severity of the thermal stress...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... in the region encoding the N-terminal portion of the protein (including the signalpeptide and small tandem repeats), a large exon com-prises the middle region of the ORF, which encodes the ... morphologies havebeen discussed. These isolated single proteins havesome activity in changing crystal morphologies; how-ever, analyses of the single proteins has not ledto insights into how these ... containing differentdomains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing...
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... adetailed understanding of the biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding theseenzymes. The biosynthesis ... olean-3b,24-di-acetoxy-12-ene, except for the presence of several back-ground peaks (the amount of authentic sample wasadjusted to equalize the height of both peaks in GC).When the extract was incubated with sophoradiol, ... isolated from the plant itself, but they wereidentified as the products of heterologously expressedtriterpene synthases [10,14,17,20,24]. Although the presence of some triterpenes (lupeol, b-amyrin,...
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