Báo cáo khoa học: Allosteric modulation of Euphorbia peroxidase by nickel ions pdf

... lost.The effect of Ni2+in the absence of added CaCl2was determined by inverse plots of the steady-statekinetics at saturating concentrations of ABTS (10 mm) Nickel ions and Euphorbia peroxidase ... (2008) 1201–1212 ª 2008 The Authors Journal compilation ª 2008 FEBS Allosteric modulation of Euphorbia peroxidase by nickel ions Francesca Pintus1, Anna Mura1, Andrea Bellelli2, Alessandro ... ascorbate peroxidase and cyto-chrome c peroxidase. Class II consists of secretedfungal peroxidases, e.g. manganese peroxidase andlignin-degrading peroxidase. Class III containssecreted plant peroxidases,...

Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

... presence of saturating concentrations of myristate, the tendency of the curve to become sig-moidal is much attenuated, suggesting a substantialimpairment of allosteric modulation by myristate ... state HSA transition, and appear of generalsigniﬁcance for a deeper understanding of the allosteric modulation of ligand binding properties of HSA.AbbreviationsFA, fatty acid; HSA, human serum ... core of the model was based on the competitionbetween Mn(III)heme and myristate for binding toFA1 (deﬁned by the parameters KHand KM) and onthe allosteric modulation of FA1 properties by...

Báo cáo khoa học: Dual modulation of prothrombin activation by the cyclopentapeptide plactin pptx

... included to inhibit the feedback proteolysis of prothrombin by the generated thrombin). The reaction wasstopped by the addition of an equal volume of acetic acid,and the resulting mixture was ... model.Therefore, the dual modulation of prothrombin activation by plactin maybe interpreted as leading to anticoagulation under physiological coagulat-ing conditions.AbbreviationsDAPA, dansylarginine-N-(3-ethyl-1,5-pentanediyl)amide; ... presence of aprotinin, an inhibitor of plasmin. Line indicatesthe average of duplicate determinations. (B) U937 cells were incu-bated with125I-labeled scu-PA in the absence or presence of 50...

Báo cáo khoa học: The modulation of metal bio-availability as a therapeutic strategy for the treatment of Alzheimer’s disease pptx

... development of effective therapeutic strategiesis hampered by a paucity of information on the biolo-gical mechanisms underlying the disease. The severity of AD relates to a multitude of age-related ... that the loss of hippocampalsynapses in rat organotypic slices is mediated by Aboligomers and is dependent on the activity of NMDA-type glutamate receptors.The concentrations of metal required ... that Ab-mediated inhibition of cyto-chrome c oxidase requires the presence of at leastequimolar concentrations of Cu [66], and that theinhibition was not supported by Zn or Fe [67]; andHuang...

Báo cáo khoa học: Allosteric functioning of dimeric class C G-protein-coupled receptors doc

... soluble VFTs [32]. However, this may beexplained by the absence of the other part of thereceptor (the CRD and the HD), as well as by theabsence of cations that stabilize the Acc state.Although ... functioning of class C GPCRs and illustrate how allosteric inter-actions between the subunits play a fundamental rolein their activation. Of interest, we see that this com-plex functioning of class ... crystal structure of the dimers of mGlu1 VFTs[9,10] (Fig. 2A). Mutation of the Cys residue involvedin the covalent linkage of the subunit also does notaffect functioning of the receptor [22]....

Báo cáo khoa học: "The Modulation of Cooperation and Emotion in Dialogue: The REC Corpus" pdf

... been approved by the Human Research Ethics Committee of Trento University. REC is by now made up of 17 dyadic interac-tions, 9 with confederate, for a total of 204 min-utes of audiovisual ... predic-tions are made on the speaker role. 4 Conclusions and Future Directions Cooperative behavior and its relationship with emotions is a topic of great interest in the field of dialogue ... emotions and, as a result, having a clearer idea of facial expression of emotions in dialogue. In fact, REC is created to shade light on the relationship between cooperation and emotions...

Báo cáo khoa học: Selective modulation of protein C afﬁnity for EPCR and phospholipids by Gla domain mutation pdf

... (1994)Functions of individual gamma-carboxyglutamic acid(Gla) residues of human protein C. Determination of functionally nonessential Gla residues and correlationswith their mode of binding ... of protein C fromsEPCR was achieved by injection of HBS-EP buffer, containing3mM EDTA. 3, Injection of 10 mM glycine ⁄ HCl pH 2.5 regeneratedthe antibody surface by the complete removal of ... a response of 3600 RU. (Upper) Binding of protein C to wild-type sEPCR. Approximately 400 RU of sEPCRwas captured before sequential injections containing decreasingconcentrations of protein...

Tài liệu Báo cáo khoa học: Reaction mechanisms of thiamin diphosphate enzymes: redox reactions pdf

... channel of E1 suchthat (a) formation of 8-(S)-acetyl-dihydrolipoylamideis more likely than of the 6-S isomer and (b)protonation of the lipoamide dithiolane by His407K. Tittmann Redox reactions of ... conducted by Frey et al. onthe E2-catalyzed acetylation of free dihydrolipoamide by acetyl-CoA clearly revealed formation of the 8-Sisomer, followed by non-enzymatic isomerization andformation of ... (i.e.deprotonation of C2a-OH of HEThDP and proton-ation of FAD at N5 and N1). The tight and rigidbinding of both cofactors excludes a direct carbanionmechanism with covalent linkage between C2a of theHEThDP...

Tài liệu Báo cáo khoa học: Metabolic control of mitochondrial properties by adenine nucleotide translocator determines palmitoyl-CoA effects Implications for a mechanism linking obesity and type 2 diabetes pdf

... adversely affect cellular function by directeffects on a variety of enzymes and induction of apoptosis [4].Tight regulation of intracellular concentrations of free LCACs by acyl-CoA-binding protein ... concentrations and Dw [16].The relative contribution of a particular enzyme to thecontrol of metabolic pathway ﬂux and concentrations of reaction intermediates determines to what extentinhibition of ... the control of ﬂuxes and inter-mediates of oxidative phosphorylation not only by theANT but also by other components of oxidative phos-phorylation. Furthermore, we tested parts of the abovehypothesis...

Tài liệu Báo cáo khoa học: Improving Classification of Medical Assertions in Clinical Notes" pdf

... Classification of Medical Assertions in Clinical Notes Youngjun Kim Ellen Riloff Stéphane M. Meystre School of Computing School of Computing Department of Biomedical Informatics University of ... more efficient ways to process increasingly large collections of clinical narratives. As evidence of this transition, nearly all of the best-performing systems in the Fourth i2b2/VA Challenge ... includes 349 clinical notes, with 11,967 assertions of medical problems. The test set includes 477 texts with 18,550 assertions. These assertions were distributed as follows (Table 1): ...

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