Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

... the metabolism of d-Asp in the plant by catalyzing trans- amination between d-amino acids. This is the first report of cDNA cloning and functional characterization of a d-amino acid aminotransferase ... particularly for d-amino acids, were characterized. Gene cloning and functional characterization of amino acid AT recombinant proteins from A. thaliana Asp...

Ngày tải lên: 30/03/2014, 04:20

... 0.3 18:2 D9,12 – – 58.3 ± 0.1 43.5 ± 0.6 18:3 D6,9,12 – – – 11.1 ± 0.8 18:3 D9,12,15 42.6 ± 1.2 36.7 ± 1.9 – – 18:4 D6,9,12,15 – 5.2 ± 0.1 – – 20:2 D11,14 – – – 2.9 ± 0.1 20:3 D8,11,14 – – – 1.7 ± 0.1 ARA – ... 2.9 ± 0.1 20:3 D8,11,14 – – – 1.7 ± 0.1 ARA – – – 0.17 ± 0.03 20:3 D11,14,17 – 4.7 ± 0.6 – – 20:4...

Ngày tải lên: 31/03/2014, 09:20

... paper describes the cloning and functional characterization of an AK gene from a genomic library of L. donovani as well as its expression and molecular characterization. Materials and methods Materials Plasmids ... transfer of the c-phosphate group from ATP to AMP, releasing two molecules of ADP. This study describes cloning and functional characterization of t...

Ngày tải lên: 21/02/2014, 00:20

... excitability and associative learning. J Neurosci. 23, 70 0–7 07. 2830 A. Pisarchik and A. Slominski (Eur. J. Biochem. 271) Ó FEBS 2004 Molecular and functional characterization of novel CRFR1 isoforms from ... with deletion of exon 11, 27 basepairs of exon 10 and 28 basepairs of exon 12 (AF369653); and CRFR1h with addition of a cryptic exon (AF374231) [1 5–1 8]. I...

Ngày tải lên: 07/03/2014, 15:20

... Journal 272 (2005) 409 1–4 102 ª 2005 FEBS Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis Melissa ... Druker BJ, Tsygankov A, Band V & Band H (2003) Cbl-mediated ubiquitinylation and negative regulation of Vav. J Biol Chem 278, 3849 5–3 8504. Molecular characterizatio...

Ngày tải lên: 07/03/2014, 21:20

... protein composed mainly of a helices (Figs 1 and 2). The core of Iba2 is a pair of EF-hand motifs, denoted as EF-hands 1 and 2, each consisting of two a helices (aA, aB and aC, aD, respectively) ... may be concluded that EF-hand 1 of the Iba proteins is not capable of functional Ca 2+ binding. Function of EF-hand 2 In both Iba1 crystal structures, the loops of EF-hand...

Ngày tải lên: 16/03/2014, 06:20

... results of the luciferase activity assays for different constructs in A549 and DU145 cells. (C) The effects of 2 lg of the NEG-U decoy, 2 lg of the NEG-D decoy and a combination of 1 lg of each ... indicated above each lane. (B) Activities of F-233 and MF-233 in A549 and DU145 cells. (C) Activities of F-233 and varying amounts of the decoy oligonucleotides in A549...

Ngày tải lên: 30/03/2014, 04:20

... prolifera- tive and differentiation state, basal level of ERK1 ⁄ 2 and Akt kinase phosphorylation, and P2Y 1 and P2Y 12 receptor protein expression and functional activity. Examination of the effects of specific ... characterized the expression and functional properties of the ADP- sensitive P2Y 1 and P2Y 12 nucleotide receptors in glioma C6 cells cultured in medium d...

Ngày tải lên: 30/03/2014, 08:20

... HPRT A B C D PRTFDC1 PRTFDC1 HPRT HPRT 0.4 0.3 V (µmol·min –1 ·mg –1 ) V (µmol·min –1 ·mg –1 ) V (µmol·min –1 ·mg –1 ) V (µmol·min –1 ·mg –1 ) 0.2 0.1 0.0 0 50 100 150 –5 0 200 400 600 800 1000 0 50 100150 200 250 S –5 0 0 2 4 6 8 10 12 50 ... activity [10]. The structural characterization of numerous com- plexes of the human HPRT and several bact...

Ngày tải lên: 15/02/2014, 01:20

... [27]. The three SGLs of HYD Js , i.e. SGL1, SGL2 and SGL3, correspond to residues 6 0–7 1, 9 1–9 9 and 15 1–1 61, respectively. On the basis of the homol- ogy model, the SGLs of HYD Js and HYD Bp (PDB accession ... the production of d-amino acids [20]. The structure of HYD Bp was also determined, and its catalytic active site was found to consist of two metal ions...

Ngày tải lên: 18/02/2014, 08:20