Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf

... the metabolism of d-Asp in the plant by catalyzing trans-amination between d-amino acids. This is the ﬁrst report of cDNA cloning and functional characterization of a d-amino acid aminotransferase ... particularly for d-amino acids, werecharacterized.Gene cloning and functional characterization of amino acid AT recombinant proteins fromA. thaliana AspATSix A. thaliana AspAT clones (Atasp 1–5 and prokary-otic-type ... compilation ª 2008 FEBS Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination Miya Funakoshi1,*, Masae Sekine1,*, Masumi...

Báo cáo khoa học: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis doc

... 0.318:2D9,12 – – 58.3 ± 0.1 43.5 ± 0.618:3D6,9,12 – – – 11.1 ± 0.818:3D9,12,1542.6 ± 1.2 36.7 ± 1.9 – – 18:4D6,9,12,15 – 5.2 ± 0.1 – – 20:2D11,14 – – – 2.9 ± 0.120:3D8,11,14 – – – 1.7 ± 0.1ARA – ... 2.9 ± 0.120:3D8,11,14 – – – 1.7 ± 0.1ARA – – – 0.17 ± 0.0320:3D11,14,17 – 4.7 ± 0.6 – – 20:4D8,11,14,17 – 1.5 ± 0.2 – – EPA – 0.23 ± 0.03 – – 4110 F. Domergue et al. (Eur. J. Biochem. 269) ... insensitivity of front-end desaturases and elongases to the presence of an x3-double bond and of x3-desaturases to the structure of the carboxyl end of thefatty acids, the existence of separate x3- and...

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... paper describes the cloning and functional characterization of an AK gene froma genomic library of L. donovani as well as its expression and molecular characterization. Materials and methodsMaterialsPlasmids ... transfer of the c-phosphate group from ATP toAMP, releasing two molecules of ADP. This study describes cloning and functional characterization of the gene encodingAK2 from a genomiclibrary of Leishmania ... RNAtranscript of  1.9 kb. Transcription of the AK gene washigher during early period (days 1 and 2) of growth,Fig. 1. Multiple amino acid sequence align-ments of L. donovani AK. The predicted aminoacid...

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... excitability and associative learning. J Neurosci. 23,70 0–7 07.2830 A. Pisarchik and A. Slominski (Eur. J. Biochem. 271) Ó FEBS 2004Molecular and functional characterization of novel CRFR1 isoformsfrom ... with deletion of exon 11, 27 basepairs of exon 10 and 28 basepairs of exon12 (AF369653); and CRFR1h with addition of a crypticexon (AF374231) [1 5–1 8]. In rodents, three CRFR1isoforms have already ... isoform where exon 3 and exon 6 are spliced out (U16273); CRFR1d isoform whereexons 6 and 13 are missing (AF180301); CRH-R1e withdeletion of exons 3 and 4 (AF369651); CRFR1f withdeletion of...

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... Journal 272 (2005) 409 1–4 102 ª 2005 FEBSMolecular and functional characterization of a novel splicevariant of ANKHD1 that lacks the KH domain and its rolein cell survival and apoptosisMelissa ... Druker BJ, Tsygankov A,Band V & Band H (2003) Cbl-mediated ubiquitinylation and negative regulation of Vav. J Biol Chem 278,3849 5–3 8504.Molecular characterization of ANKHD1 splice variant ... are coded by these speciﬁc exons.VBARP-L and VBARP-S code for precursor pro-teins of 627 and 435 amino acids and with calculatedpeptide masses of 69 and 49 kDa, respectively. Addi-tional domain...

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

... protein composedmainly of a helices (Figs 1 and 2). The core of Iba2 is apair of EF-hand motifs, denoted as EF-hands 1 and 2,each consisting of two a helices (aA, aB and aC, aD,respectively) ... may be concluded that EF-hand 1 of the Ibaproteins is not capable of functional Ca2+binding.Function of EF-hand 2In both Iba1 crystal structures, the loops of EF-hand 2adopt essentially the ... Res 307, 20 4–2 11.26 Gifford JL, Walsh MP & Vogel HJ (2007) Structures and metal-ion-binding properties of the Ca2+-binding helix–loop–helix EF-hand motifs. Biochem J 405, 19 9–2 21.27 Brodersen...

Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx

... results of the luciferaseactivity assays for different constructs in A549 and DU145 cells.(C) The effects of 2 lg of the NEG-U decoy, 2 lg of the NEG-Ddecoy and a combination of 1 lg of each ... indicatedabove each lane. (B) Activities of F-233 and MF-233 in A549 and DU145 cells. (C) Activities of F-233 and varying amounts of thedecoy oligonucleotides in A549 and DU145 cells. WP-decoy indi-cates ... FEBS 1873 Cloning and functional analysis of 5¢-upstream region of the Pokemon geneYutao Yang, Xiaowei Zhou, Xudong Zhu, Chuanfu Zhang, Zhixin Yang, Long Xu and Peitang HuangLaboratory of Protein...

Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

... prolifera-tive and differentiation state, basal level of ERK1 ⁄ 2 and Akt kinase phosphorylation, and P2Y1 and P2Y12receptor protein expression and functional activity.Examination of the effects of speciﬁc ... characterized the expression and functional properties of the ADP-sensitive P2Y1 and P2Y12nucleotide receptors in glioma C6 cells culturedin medium devoid of serum for up to 96 h. During this long-term ... Jolanta Baran´skaDepartment of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, PolandThe focus of this study is glioma C6 cells,...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... HPRTABCDPRTFDC1PRTFDC1HPRTHPRT0.40.3V (µmol·min –1 ·mg –1 )V (µmol·min –1 ·mg –1 )V (µmol·min –1 ·mg –1 )V (µmol·min –1 ·mg –1 )0.20.10.00 50 100 150 –5 020040060080010000 50 100150 200 250S –5 0 02468101250 ... activity [10].The structural characterization of numerous com-plexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [1 3–1 7]. Thestructure of human HPRT can be divided ... 602.012002520151050100080060040020001.51.00.50.0Fig. 3. Characterization of PRTFDC1 and HPRT with hypoxanthine and guanine.Michaelis–Menten and Hanes–Woolf plotsillustrate the kinetic pattern. The...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... [27]. The three SGLs of HYDJs, i.e. SGL1,SGL2 and SGL3, correspond to residues 6 0–7 1, 9 1–9 9 and 15 1–1 61, respectively. On the basis of the homol-ogy model, the SGLs of HYDJs and HYDBp(PDBaccession ... theproduction of d-amino acids [20]. The structure of HYDBpwas also determined, and its catalytic activesite was found to consist of two metal ions and sixhighly conserved amino acid residues. ... [39]. Cloning and expression of putative HYDsThe genomic DNA of Jannaschia sp. CCS1 and Pseudomo-nas ﬂuorescens PfO-1 were kindly supplied by Mary Moran(University of Georgia, Athens, GA) and...

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