Báo cáo khoa học: Purification and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

... be explained by the broader substrate-binding spectrum of bCYP11B1 resulting from the combination of the functions of CYP11B1 and CYP11B2 within one enzyme. Comparison of the k cat values obtained ... volume and energetic properties of the binding of CO to hemoproteins. Biophys J 66, 89–98. 42 Tuckey RC & Kamin H (1983) Kinetics of O 2 and CO Binding to adrenal cytochr...

Ngày tải lên: 30/03/2014, 04:20

... toxin, with a molecular mass of 34 kDa, was identified as sphingomyelinase C (EC 3.1.4.12) on the basis of its N-terminal and internal amino-acid sequences. A recombinant sphingomyelinase C expressed ... sequencing the N-terminal part of a peptide fragment obtained by tryptic digestion of the protein. Cloning and sequencing of the insecticidal toxin ( SMC ) gene of B. cereus...

Ngày tải lên: 30/03/2014, 13:20

... superimposed structures of conomarphin (red) and L-Phe13-conomarphin (gray). The side chain of L-Phe13 might cause spatial hindrance to the side chain of Lys9 and Hyp10 in forming the tight loop as in conomarphin. ... side chain of Lys9, Hyp10 and D-Phe13 of cono- marphin is shown in stick mode, and the side chain of L-Phe13 in L-Phe13-conomar- phin is shown as a g...

Ngày tải lên: 18/02/2014, 17:20

... polycrystalline gold and alkylated gold. The arrows and thick arrows indicate the starts of sample loading (2 l M) and washing by the running buffer, respectively. The processes of sample loading and ... space of several organ- isms, including bacteria and fish [17], the Ala and ⁄ or methyl group of Thr on the molecular surface of the protein are known to be essential in...

Ngày tải lên: 16/03/2014, 05:20

... (2006) Microfilament-binding properties of N-terminal extension of the isoform of smooth muscle long myosin light chain kinase. Cell Res 16, 367–376. Aggregation domain in myosin light chain kinase W C. ... Nanjing University, China 2 School of Life Science, Nanjing Normal University, China 3 National Institute of Biological Science, Beijing, China Myosin light chain kinases (MLCKs...

Ngày tải lên: 30/03/2014, 04:20

... of prostaglandins [2]. Apart from their metabolic activities, they are involved in the detoxication of electro- philic and genotoxic compounds by both catalytic activity and direct binding. In ... were injected in a 500-lL loop valve with bracketing, a method which improved the resolution and increased the peak heights according to Guinn and Hendricx [18]. N-Terminal amino a...

Ngày tải lên: 31/03/2014, 09:20

... the GHF9-cellulase possessing a family II CBM in the N-ter- minal region. By genomic PCR using specific primers to the 3¢-terminal coding sequences of HdEG66-cDNA, a DNA of 2186 bp including three introns was ... three cysteines are however present at residues 33, 58, and 90. In addition, a putative linker region rich in threonine and glycine residues locates in the position co...

Ngày tải lên: 20/02/2014, 23:20

... protein gave a pink color on PAS staining (data not shown), indicating it was a gly- coprotein. The yield of purified Cyn d 24 was < 0.1% of total soluble BGP protein. Lectin-binding activity of ... Carbohydrate staining was performed using a Glycoprotein staining kit (Pierce, Rock, IL, USA) accord- ing to the manufacturer’s instruction. Lectin-binding assays The ability of Cyn d 24...

Ngày tải lên: 07/03/2014, 12:20

... Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor Rong Jin 1 , Linsen Dai 1 , Jinbiao Zheng 1 and Chaoneng Ji 2 1 Center of Analysis and Measurement ... of Fig. 4A, are shown in Fig. 6 and Table 2. From Table 2, the sum of individual amide I¢ intensity and the intensity percent of each peak from human Fig. 3. Activity...

Ngày tải lên: 07/03/2014, 15:20

... sequence linked to a protein. The a2,6-linkage was confirmed by the increase of Sambucus nigra agglutinin- lectin binding to the cell surface of CHO transfected with the cDNA encoding ST6Gal II and ... oligo- saccharides on lutropin, follitropin, and thyrotropin. I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glyc...

Ngày tải lên: 08/03/2014, 08:20