Báo cáo khoa học: Modulation of P-glycoprotein-mediated multidrug resistance by acceleration of passive drug permeation across the plasma membrane potx

... permeation of drugs across the plasma membrane. Thus, reversal of multidrug resistance (MDR) can occur either by inhibition of the efflux pumps or by acceleration of the drug per- meation. Among the ... of the anesthetics benzyl alcohol, propofol, chlo- roform and diethyl ether reversed the resistance by acceleration of the passive transport...

Ngày tải lên: 30/03/2014, 04:20

... under these conditions, the free concentration of the dyes in the cytoplasm is low compared to the extracellular concen- trations of the dyes. Equilibration of the dyes across the plasma membrane ... in the cytoplasm with their concentration in the exter- nal medium. The protection provided to multidrug resistance cells by ABC transporters has to overcome...

Ngày tải lên: 18/02/2014, 13:20

... the changes in free energy of unfolding were because of changes in the gradient of the urea depen- dence of the free energy (supporting information), rather than changes to the mid-point of the ... triplicate. Determination of free energy of unfolding Equilibrium unfolding of the proteins and their deriva- tized glycoforms was monitored in the presence of ure...

Ngày tải lên: 15/03/2014, 11:20

... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... Figure 4A shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 lm dopamine on the aggregation process. Dopamine...

Ngày tải lên: 14/02/2014, 19:20

... in rupture of the outer membrane. The identity of the proteins comprising the PTP is debated; the ubiqui- tous matrix-located protein cyclophilin D (CYPD) is involved in the modulation of PTP open ... setting, the ability of the F 0 F 1 -ATP synthase to pump protons out of the matrix represents the only component opposing the action of an uncoupler. On th...

Ngày tải lên: 14/02/2014, 19:20

... affect sterol biosynthesis dur- ing the formation of a mating projection. Nevertheless, the phenotypes of mutants defective in ergosterol synthesis clearly demonstrate the importance of sterols for ... cleave off the reporter RUra3, which is fused to the PAK. Released RUra3, a modified version of the enzyme Ura3 with an arginine at the extreme N-terminus, is targeted for...

Ngày tải lên: 18/02/2014, 13:20

... result of changes introduced by the mutations in the environment of the prosthetic group. As a result of the important changes observed for each mutant in the differential spectra elicited by NADP + , ... in the protein stability of our mutants. There- fore, these conclusions strengthen our hypothesis that the catalytic efficiencies of the recombinant enzymes used...

Ngày tải lên: 18/02/2014, 17:20

... During the subsequent period, the integration of ‡ 2 units of the transfected plasmid into the parasite genome seemed to occur, as shown by the production of both DNA segments after ‡ 4 weeks of ... negli- gible values [7,9,10]. These results could be attributed neither to a deficient uptake of the amino acids by the parasites [12] nor to the presence of ODC or...

Ngày tải lên: 19/02/2014, 07:20

... action of EPA and DHA (Eur. J. Biochem. 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic ... to the S phase. Expression of the cyclin D1 gene seems to be regulated essentially at the transcription level. It is blocked by inhibitors of the MAPK or P...

Ngày tải lên: 19/02/2014, 16:20

... and through modulation of the effects of co-repressors on its repression domains. These are the first results to indicate that the structural components of GRIP1, especially those of the C terminus, ... obvious sequence homology, the nature of their downstream targets and the specific components of the transcriptional machinery remain unknown. The mechanisms by w...

Ngày tải lên: 07/03/2014, 12:20