Báo cáo khoa học: Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC doc

... by expressing the conserved region of yeast Cox18 in E. coli, we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the ... approach in E. coli. The data demonstrate that Cox18 complements the essential Sec-independent function of YidC. Hence, by this criterion, Co...

Ngày tải lên: 30/03/2014, 03:20

... related proteins from other fungi, or even in the fission yeast Schizosaccharomyces pombe. Both the lack of obvious orthologs in other fungi and the abundance of Om14 in the outer mem- brane would ... biogenesis. While the function of Om14 remains unclear, it may be related functionally to another major protein in the outer membrane, Om45. No detailed data on the stoi- ch...

Ngày tải lên: 19/02/2014, 07:20

... the semiquinone at the P-site in the presence of antimycin A or my- xothiazol. The semiquinone formed in the presence of myxothiazol is the result of a slow leak of electrons to the iron–sulfur ... understand the function of Coq10p, we tested the reducibility of Q 6 in a coq10 null mutant in the presence of inhibitors that block Q 6 binding to the P (o)-s...

Ngày tải lên: 06/03/2014, 22:21

... been implicated in all but one of the predicted reactions in the GPI pathway. The exception is the addition of the second mannose to the GPI core. We show here that depletion of the essential, multispanning ... conclude that human Ybr004cp is the functional equivalent of S. cerevisiae Ybr004cp. Discussion The majority of the steps in assembly and decoration o...

Ngày tải lên: 07/03/2014, 16:20

... recognized by the Pex7p peroxin [27,28]. The two receptors bind their cargo proteins in the cytosol and recruit them to the peroxisomal membrane by interacting with the core com- ponents of the docking ... genes are the focus of our study. The exact function of Pex25p is not yet known, but its absence from cells grown on oleic acid medium appears to affect the extent...

Ngày tải lên: 17/03/2014, 03:20

... previous work, the role of a-mannosidase was studied by examining the effect of disruption of the MNS1 gene encoding ER a-mannosidase on glycosylation, and the results suggested that the mannose ... not into the oligomannose acceptors that are recognized by other mannosyltransferases like Kre2p ⁄ Mnt1p. These results support further understanding of the molecular mech- a...

Ngày tải lên: 23/03/2014, 10:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... that the enzy- matic activity of RPE65c is iron-dependent. Characterization of the kinetic parameters of the enzymatic activity of zebrafish RPE65c To determine the steady-s...

Ngày tải lên: 14/02/2014, 14:20

... roles may include the stabilization of the positive charge on the methyl carbon of SAM, or the enhancement of the negative charge on the propionate carboxyl groups of MgD. The rate constants ... timepoint. The data for the decay of inter- mediate in the presence of ChlH were fitted to a three-parameter exponential (k ¼ 31.7 ± 9.5 s )1 ). The evolution of int...

Ngày tải lên: 20/02/2014, 02:21

... in the expression of the corre- sponding genes [17,65]. Therefore, the extent of the physical involvement of the two receptors, atToc33 and atToc159, in the translocation of the photosyn- thetic ... Toc159, the existence of which is contested. Despite the differences between the two models, there is a strong consensus on the composition of the Toc core comp...

Ngày tải lên: 07/03/2014, 03:20

... Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps. Residues present in the flexible region of the C-terminal extension of each of the eight ... examined the role of residues in the C-terminal extensions of other sHsps, notably aA- and aB-crystallin, the flexible regions of these proteins are unique and...

Ngày tải lên: 07/03/2014, 04:20