Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt

... calcium and ATP FEBS Journal 274 (2007) 4561–4571 ª 2007 The Authors Journal compilation ª 2007 FEBS 4571 Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor ... mixture. Characterization of the activation of brain SR by GRIP(468–1112) We next analysed whether larger ratios of PDZ6 domain: SR might b...

Ngày tải lên: 30/03/2014, 03:20

... characterized by the presence of a vinyl ether substituent at the sn-1 position of the glycerol backbone. They are especially abundant in electrically active tissues, such as brain, where most of them ... on the type of PLA 2 , this cleavage leads to their inactivation (in the case of the cytosolic PLA 2 ,typeIV, without arachidonate-phospholipid remodeling activity)...

Ngày tải lên: 08/03/2014, 08:20

... and poppy [20,21]. In the present study, the first insights into the secon- dary and tertiary structure of PLD a2 from cabbage are given. The surface structure of the enzyme was investigated by ... of the enzyme. These results unambiguously show that activation of PLDa2 by calcium ions is due to the binding of cal- cium ions to the enzyme, and not (or...

Ngày tải lên: 19/02/2014, 02:20

... corrected by substracting the spectrum of the buffer solution (5 mm Tris ⁄ HCl, pH 7.5) in the absence of protein. The stability to thermal inactivation was examined by incubation of the enzymes ... explained by elec- trostatic repulsion between the a-carboxyl group of the amino acid and the introduced aspartic residue at position 149. On the other hand, as a...

Ngày tải lên: 20/02/2014, 02:21

... analysis of initial rate data in the absence of inhibitors, we used the specific equation in Scheme 1. The effect of two ligands on the activity of the wild-type DmAChE was evaluated by the equation ... used 1 and 10 lm propidium and 10 and 50 lm aflatoxin. The activity was followed for 1 min after the addition of acetylcholinesterase to the mixture, and t...

Ngày tải lên: 07/03/2014, 05:20

... consumption of FNR sq by the second process, the same amount of FNR sq is produced by the first one. This is consistent with the high K d values proposed for the interaction of FNR and Adx and with the ... the changes introduced in the redox cofactor environment upon complex formation. They are therefore related to the specificity and the strength of the...

Ngày tải lên: 31/03/2014, 07:20

... fact, the bulk of the proteolytic processing of the proforms of the SVMPs occurs in advance of release of mature secre- tory vesicle contents into the lumen of the venom gland. In the case of the ... associated with the structure of the adjacent proteinase and disintegrin-like domains and the pack- ing of those two domains together in the struct...

Ngày tải lên: 18/02/2014, 18:20

... classify these instances based on factors leading to non-projectivity and present our analysis of them. For each of these classes, we look at the rigidity of these non-projective constructions and their ... is a chunk and the edge represents the relations be- tween the connected nodes labeled with the karaka or other relations. All the modifier-modified rela- tions betwe...

Ngày tải lên: 20/02/2014, 09:20

... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 in Fig. 5) defines the allowable movements of the FMN subdomain and thus controls the FMN–NOSoxy ... also influence the rate of electron import into the FMN sub- domain and the resulting formation of FMNH 2 . The extent to which K eq A or the rate of...

Ngày tải lên: 18/02/2014, 11:20

... evidence for an interaction between the two domains is the ability of numerous substrates and modulators of ABCG2 (and the R482G isoform) to stimulate the rate of ATP hydrolysis [21,24,25], albeit ... observed in the presence of the ATP analogues ATP- c-S (nonhydrolysable) and TNP -ATP (slowly hydrolysable), although the magnitude of effect with the latter...

Ngày tải lên: 18/02/2014, 18:20