Báo cáo khoa học: Activation of cathepsin D by glycosaminoglycans docx

... showed that similar to pseudoCD, mature CD can be stimulated by low concentrations of MH. The observation that the amount of activation of pseudoCD and two-chain CD by MH was similar indicated that ... addition of MH. Because pseudoCD lacks residues 1–26 of the prodomain, the result suggested that other regions of the enzyme were required for complete activation of proCD...

Ngày tải lên: 30/03/2014, 02:20

... exopeptidase activity of cathepsin B in the presence of 2A2 mAb. Furthermore, 2A2 mAb also inhibited cathepsin B endopeptidase activity, as determined by the degradation of DQ-col- lagen IV and BODIPY ... (A), degradation products are visible intracellularly and pericel- lularly (white arrow). Addition of the 2A2 mAb to the assay medium reduced the degradation of DQ-collagen I...

Ngày tải lên: 18/02/2014, 11:20

... shown). Table 2. Effects of PTMA and dTC on WT and mutant receptors. Data were analyzed as described in the legend to Fig. 3. K I values were determined as described in Experimental procedures. Each experiment ... to the a1 and b1 subunits in the nomenclature recommended by the International Union of Pharmacology [3]. Radioligand binding studies have demonstrated that, under equilib- ri...

Ngày tải lên: 19/02/2014, 07:20

... directly fused at the C-terminus of M 2 .Amyc-epitope tag was added at the N-terminus of M 2 .To prevent rapid degradation, the central part of the third intracellular loop of M 2 was deleted. ... 5¢-TTACCTTGTAGCG CCTATGTTCTTATAATG-3¢. (An engineered EcoRI recognition site is single-underlined. The start codon is double-underlined. The modified original start codon of M 2 is dot-unde...

Ngày tải lên: 07/03/2014, 11:20

... seeded in 96-well assay plates (5000 cells per well). After 18 h of subculture, the fetal bovine serum-supplied medium was removed and replaced by a double volume of the washing medium, as indicated ... diacetate and 0.5 mgÆmL )1 of propidium iodide dissolved in dimethylsulfoxide and placed in a Goryaev chamber. Cells were visualized in the dark field using a fluorescent DMLS microscope...

Ngày tải lên: 07/03/2014, 11:20

... °C, followed by washing and addition of 200 lL of anti-CAT-DIG working solution. The MP modules were further incubated for 1 h at 37 °C and re-rinsed. Two hun- dred microliters of anti-digoxigenin-peroxidase ... shifted bands corresponding to two different forms of CRE DNA- binding complexes were detected. ATF-2-CRE DNA binding activity was markedly induced following 8-h hA treatmen...

Ngày tải lên: 07/03/2014, 12:20

... B, Radel JD, Carlson SE (2004) Decreased brain docosahexaenoic acid during development alters dopam- ine-related behaviors in adult rats that are differentially affected by dietary remediation. ... high abundance in the gland, ratio 2 represents the status of the most studied polyunsatu- rated n-6 arachidonic acid (AA) and n-3 EPA, DPA, and DHA. There is a 2.9-fold and 2.1-fold decrease ....

Ngày tải lên: 16/03/2014, 10:20

... limited, and was observed in LNCap. Interestingly, DLD-1 derived from colon can- cer also expressed low level of KLK3. KLK4 was abundantly expressed by LNCap and MRT-1, and to a lesser degree by DLD-1. ... pro- HGFA, and the addition of phosphoramidon completely inhibited the activity of thermoly- sin. Processing of pro-HGFA was deter- mined by SDS ⁄ PAGE under reducing conditio...

Ngày tải lên: 23/03/2014, 07:20

... was based on the method of Michaelis et al. [22], and modified for optimal results. All procedures and treatments for hand- ling animals were reviewed and approved by the Comite ´ de Etica of CONICET ... Co ´ rdoba Ciencia (Gobierno de la Provincia de Co ´ rdoba), Secretarı ´ a de Ciencia y Te ´ cnica de la Universidad Nacional de Co ´ rdoba y Secretarı ´ ade Ciencias de la Universidad N...

Ngày tải lên: 23/03/2014, 07:20

... well described by a two-binding-site model [13], and we proposed that the high-affinity and low- affinity adsorption can be interpreted as productive and nonproductive binding, respectively, based ... the two-domain structure of cellobiohydrolase and the q dependence of cellobiose production [14]. In that study, we mainly focused on the K ad1 values to explain the different hydrolytic rat...

Ngày tải lên: 23/03/2014, 09:21