Báo cáo khoa học: Identification and characterization of the metal ion-dependent L-alanoyl-D-glutamate peptidase encoded by bacteriophage T5 pdf

... Asp130, and His133) that are typical for metallopeptidases of the M15 family and take part in the binding of the metal ion in the process of catalysis [28]. This is the first example of an l-alanoyl-d-glutamate peptidase ... 2009 FEBS are the closest to bacteriophage T5 peptidase; they are encoded by the genomes of bacteriophages ST64T, PS3, phiEcoM,...

Ngày tải lên: 30/03/2014, 02:20

... expression of T-bet has been seen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... end of the gene, where the zebrafish and human sequences appear to be quite diverse. Together with the well-conserved gene organization, there is a conservation of...

Ngày tải lên: 16/02/2014, 09:20

... localization signal and a DNA-binding domain in the MH1 domain and the L3 loop, and the C-terminal, receptor phos- phorylation motif in the MH2 domain (Fig. 1A). The amino acid composition in the L3 loop of R-Smads provides ... properties of SmSmad1 and SmSmad1B as described above. However, in the presence of SmSmad4 and the active mutant form of TbRI, both S...

Ngày tải lên: 18/02/2014, 16:20

... SmNR1(Ile247-Ser372) and SmRXR1(Glu251-Asn376) in vitro. DNA binding of a protein containing 20 amino acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the DBD of SmNR1 (Ile247-Ser372) ... most NRs, and the length of the hinge in SmNR1 thus can be estimated to be 140 amino acids. The role of the large hinge in schistosome NR...

Ngày tải lên: 07/03/2014, 11:20

... periods of time (Fig. 3). As shown, the addition of stressors to the cul- tures inhibits growth without killing the cells. The hybridizing band in the northern analysis showed the expected size of ... represents the primary defense against O 2 • ) as suggested by its ubiquitous location in the membrane and in the cytoplasm [22], by its constitu- tive level [21]...

Ngày tải lên: 07/03/2014, 12:20

... analysis of the structure of the latter made it possible to improve the current knowledge of the mammalian PR48 cDNA. To extend these findings, the genomic structures of the human PR130 and PR48 ... different classes of cDNAs were isolated and analysed. The first is the Xenopus orthologue of PR130, the second proved to be a novel splice variant and the thir...

Ngày tải lên: 08/03/2014, 08:20

... endothelial cell surface, and is then accelerated by the reciprocal activation of FXII and PK on the surface. Zn 2+ is essential for binding of FXII and HK to a biological activating surface, and ... [55]. Expression and purification of the recombinant N-terminal region of FXII and domain D5 of HK Expression vectors for the recombinant N-terminal region of FXI...

Ngày tải lên: 16/03/2014, 05:20

... with the excep- tion of the other peaks in the cysteinylated HSA sam- ple solution. The difference of relative molecular mass of the peak d and the peak g (162.2 Da) and that of peak c and peak ... 91% with the excep- tion of the other peaks in the homocysteinylated HSA sample solution. The difference of relative molecular masses of peaks h and j (161...

Ngày tải lên: 16/03/2014, 13:20

... made on the HXPR sequence of REST and YY1 proteins. The suppressing effect of YY1 on CP2’s transcriptional activity was lost by this point mutation on the HXPR sequence of YY1 and reduced by an ... and by YY1 point mutants. The extent of the CP2–YY1 interaction was indirectly measured by assaying the transcriptional activity of CP2, because YY1 decreases t...

Ngày tải lên: 16/03/2014, 18:20

... antibody-immobilized Cb2 isoform would remain in the pellet in the absence of 8-CPT-cAMP while they would be released to the supernatant in the presence of 8-CPT-cAMP. The precipitates and supern- atants ... splice variants of Cb have been identified, as a result of alternative spli- cing of seven different exons encoding the N-terminal part of the Cb protein [5]. W...

Ngày tải lên: 16/03/2014, 18:20