Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx
Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx
... phosphorylates caspase 9 at Thr125. Recombinant His 6 caspase 9 (His–C9) or His 6 caspase 9( T125A) (both containing the catalytically inactivating C287A mutation) was incubated with recombinant ... of caspase 9 at Thr125 in vitro, these results strongly indicate that DYRK1A targets caspase 9 directly in cells. Harmine is a potent inhibitor of DYRK1A in...
Ngày tải lên: 30/03/2014, 02:20
Tài liệu Báo cáo khoa học: A zymogen form of masquerade-like serine proteinase homologue is cleaved during pro-phenoloxidase activation by Ca2+ in coleopteran and Tenebrio molitor larvae docx
... Tokyo). Antibody and immunoblotting Antibody against the Tm-mas was raised by injecting 10 lg of the purified protein into a male albino rabbit with complete Freund’s adjuvant and giving a booster injection of ... b-1,3- glucan for 60 min; lane 2, incubation with b-1,3-glucan only for 60 min; lane 3, 4, 5, 6 and 7, incubation with Ca 2+ only for 10, 20, 30, 45, 60 min, respectively; la...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Enzymatic oxidation of NADP+ to its 4-oxo derivative is a side-reaction displayed only by the adrenodoxin reductase type of ferredoxin-NADP+ reductases potx
... 4003 occupancy of the nicotinamide ring of the bound ligand in the active site [14]. In this view, it is signifi- cant that the perturbations induced by NADPO bind- ing to FprA were more intense than ... dinucleotide phosphate formation in which the initial and rate-determining step is the nucleophilic attack of the nicotinamide moiety by an active site water molecule. Wh...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... TRa1proteinwas fractionated on a polyacrylamide gel and visualized by an image analyzer. The arrow indicates the band of human TRa1protein. (C) Reporter luciferase activities induced by T3. HeLaTR and ... induced DNA fragmentation and impaired the proliferation of rat FRTL cells. This implies that T3 induces apoptosis, which is mediated by 4-1BB, and subsequent activation...
Ngày tải lên: 23/03/2014, 18:20
Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
... identified at )158 and )186, within E1b, and at ) 69 and ) 79, within E2 [4]. Moreover, in the human platelet-like K562 cell line and in human uterine tissue, transcription initiation sites were ... proteins G q and G 11 in vivo and is activated by the isoprostane 8-epi prostaglandin F2 alpha. J. Pharmacol. Exp. Ther. 281, 95 7 96 4. 36. Janssen, L.J. (2001) Iso...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf
... ( 199 6) Analysis of main chain torsion angles in proteins: prediction of NMR coupling cons- tants for native and random coil conformations. J. Mol. Biol. 255, 494 –506. 27. Wu ¨ thrich, K. ( 198 6) ... in two positions, 12 and 15, at which the native amino acids L -phenylalanine 12 and L -leucine 15 have been replaced by the nonprotein amino acids D -phenylalanine and a-amin...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1) potx
... specific binding sites for the lyn kinase [31]. These findings provide a rationale for investigating whether caspase- mediated path- wayscaninteractwithPDE5inintactcells. In this article, we show that caspase- 3 ... molecular mass of 93 98 kDa [5]. The enzyme is phosphorylated at S92 and activated by both protein kinase A and protein kinase G [6–7]. Here we explore the possibilit...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities pptx
... h at 37 °C and subsequently analyzed by SDS/ PAGE and silver staining. The metalloproteinase inhibitors EDTA, o-phenanthroline (PHEN) and TIMP-1 and the thiol protease inhi- bitor E64 and serine ... MS analysis of human GCP-2 after cleavage by gelatinase B. Human recombinant GCP-2 was analyzed by electrospray ion trap MS before (A) and after (B) incubation with activat...
Ngày tải lên: 31/03/2014, 07:20
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... [6,7], FERM and PDZ-domain-containing 2 (FRMPD2) [8] and Ras guanine exchange factor (RasGEF) very- KIND (v-KIND, or kinase noncatalytic C-lobe domain containing 1) [9] . The KIND domain in these proteins ... growth and branching of cerebellar granule cells and hippocampal neurons. Among the KIND domains in four KIND-containing proteins (Spir-2, PTPN13, FRMPD2 and v-KIND), onl...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... cell lines. When deciding between maintaining cells alive or inducing apoptosis, caspase function and activation are regulated in terms of which pathways induce cleav- age sites and modification ... and Bad bind 14-3-3, the phosphoserine ⁄ threonine binding proteins in the cytosol. 14-3-3 prevents Bax and Bad dissociation from translocating to the mitochondria by a conformati...
Ngày tải lên: 14/02/2014, 22:20