Báo cáo khoa học: Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli potx

... both of the metal-binding sites (Table 3). Although the predominant effect is on the second metal-binding site, substitution of Asp97 by glutamate and asparagine makes the process of binding of the ... 275 (2008) 6248–6259 ª 2008 The Authors Journal compilation ª 2008 FEBS 6259 Analyzing the catalytic role of Asp97 in the methionine aminopeptidase fr...

Ngày tải lên: 30/03/2014, 02:20

... determined by the different sizes of the substituents. In the case of TpT, intramolecular stack- ing of thymine residues brings the two C(5)¼C(6) bonds in close proximity, thereby increasing the ... [1] remains disputable. In view of the absolute requirement for Mg 2+ in the process of RNA synthesis by E. coli RNAP [19], related primarily to the involvement...

Ngày tải lên: 19/02/2014, 18:20

... oligosaccharide against host glycosidases during infection or could be involved in modifying the net charge of the LPS molecule [15]. However, the rationale behind the incorporation of glycine into the core ... for the -CH 2 - protons of the glycine moiety in meningococcal LPS points to an exocyclic location for the glycine residue in N. meningitidis LPS. The gen...

Ngày tải lên: 23/03/2014, 21:21

... organization of the CrPrx gene. The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of the distal and proximal histidines are indicated ... for the quantitation of microgram quantities of protein util- izing the principle of protein-dye binding. Anal Biochem 72, 248–254. 38 Laemmli UK (1970) Cle...

Ngày tải lên: 23/03/2014, 09:21

... dexamethasone is an inducer of C/EBPa, exerting a positive effect on the steady-state levels of both the mRNA and protein levels in the liver. The increase in the levels of both hepatic C/ EBPa isoforms clearly ... C/EBP responsiveness to the thymidine kinase promoter of the pT81Luc recombinant. We performed the cotransfection of CHO cells with pMSV/EBPa and sing...

Ngày tải lên: 17/03/2014, 09:20

... on the structure of the CHN enzyme E. coli KAS III and the interpretation of the structure of the E. coli KAS I– thiolactomycin complex [12], assuming a 2 A ˚ movement of H298 on malonyl-ACP binding. ... reaction. An interesting aspect of the H298E mutation is the orientation of the T300 ⁄ T302 side chains. Both have been implicated in the binding of ACP...

Ngày tải lên: 19/02/2014, 08:20

... respectively. These variations of the ratios of the metabolites suggest modifi- cations in the catalytic mechanism responsible for the metabolism of DDT. Substrate binding The substrate induced binding ... mutated positions in CYP6A2 (helix J and vicinity of the b sheet 3–3). These results are also original because this is the first description of the role of...

Ngày tải lên: 19/02/2014, 12:20

... production of inter- leukins and secretion of immunoglobulins on the mucosal surface of the intestine [49,50]. The antibacte- rial defence of the intestinal mucosa depends in part on stimulation of CCK ... euge- nin, indicating that eugenin is indeed acting through CCK 2 receptors. To further investigate the interaction of eugenin with CCK 2 receptors, we investigated...

Ngày tải lên: 19/02/2014, 16:20

... grammar, in which the subtrees are specified in advance, in an adaptor grammar the subtrees, as well as their probabilities, are learnt from the train- ing data. In order to make parsing and inference tractable ... parse of that string with a PCFG approximation to the adaptor grammar. This PCFG contains a production for each adapted subtree in the parses of the othe...

Ngày tải lên: 20/02/2014, 09:20

... contains one gene (AF500) for which no corresponding protein was found in the purified enzyme preparation. The results of the sequence analyses of the deduced proteins are given in Table 2. The ... co-ordinate three heme c molecules. AF503 shows the highest sequence similarity to a protein encoded by the dsr locus of A. vinosum, the DsrJ protein. The mature form of...

Ngày tải lên: 21/02/2014, 03:20