Báo cáo khoa học: Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli potx
Báo cáo khoa học: Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli potx
... both of the metal-binding sites(Table 3). Although the predominant effect is on the second metal-binding site, substitution of Asp97 byglutamate and asparagine makes the process of binding of the ... 275 (2008) 6248–6259 ª 2008 The Authors Journal compilation ª 2008 FEBS 6259 Analyzing the catalytic role of Asp97 in the methionine aminopeptidase from Escherichia coli Sanghamitra Mitra1, ... (2005)Charaterization of the active site and insight into the binding mode of the anti-angiogenesis agent fumagillinto the Mn(II)-loaded methionyl aminopeptidase from Escherichia coli. J Biol Inorg Chem...
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Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reflects variation in the negative electrostatic potential along the separated DNA strands ppt
... determined by the different sizes of the substituents. In the case of TpT, intramolecular stack-ing of thymine residues brings the two C(5)¼C(6)bonds in close proximity, thereby increasing the ... [1]remains disputable. In view of the absolute requirement for Mg2+ in the process of RNA synthesis by E. coli RNAP [19],related primarily to the involvement of Mg2+ in the catalysis of internucleotide ... band doubling for T)11and T+2. Therefore, the measured contribution of theseforms to the integrated intensity of these two bases wasused in the calculation of the contribution of individualbases...
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Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
... oligosaccharideagainst host glycosidases during infection or could beinvolved in modifying the net charge of the LPS molecule[15]. However, the rationale behind the incorporation of glycine into the core ... for the -CH2- protons of the glycinemoiety in meningococcal LPS points to an exocycliclocation for the glycine residue in N. meningitidis LPS. The genetic control of the elaboration of glycine ... location in the inner coreoligosaccharide as appears to be the case for N. meningitidis.Depending on the strain of H. in uenzae glycine could befound at each of the heptose residues of the inner...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... organization of the CrPrxgene. The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of the distaland proximal histidines are indicated ... for the quantitation of microgram quantities of protein util-izing the principle of protein-dye binding. Anal Biochem72, 248–254.38 Laemmli UK (1970) Cleavage of structural proteins dur-ing the ... localization of CrPrx in N. tabacum and C. roseus, the CrPrx coding regionwas fused in- frame to the coding region for the N-ter-minal side of GUS and GFP under the control of the 35S promoter of cauliflower...
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Báo cáo khoa học: Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response ppt
... dexamethasone is aninducer of C/EBPa, exerting a positive effect on the steady-state levels of both the mRNA and protein levels in the liver. The increase in the levels of both hepatic C/EBPa isoforms clearly ... C/EBPresponsiveness to the thymidine kinase promoter of the pT81Luc recombinant. We performed the cotransfection of CHO cells with pMSV/EBPa and single recombinantscontaining two or three synthetic copies of 3A1-300 ... activityWe investigated the presence and relative concentration of active C/EBP proteins in the hepatic cell nuclei as a function of the animal treatment. The results of the binding activities of nuclear...
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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... on the structure of the CHN enzyme E. coli KAS III and the interpretation of the structure of the E. coli KAS I–thiolactomycin complex [12], assuming a 2 A˚movement of H298 on malonyl-ACP binding. ... reaction.An interesting aspect of the H298E mutation is the orientation of the T300 ⁄ T302 side chains. Both havebeen implicated in the binding of ACP [2], probablyby donating hydrogen bonds to one of ... probes the roles of the active site histidines and lysine in the CHH con-densing enzyme KAS I from E. coli by use of crystalstructures of active site mutants and biochemicalcharacterization of the...
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Tài liệu Báo cáo khóa học: Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism doc
... respectively. Thesevariations of the ratios of the metabolites suggest modifi-cations in the catalytic mechanism responsible for the metabolism of DDT.Substrate binding The substrate induced binding ... mutatedpositions in CYP6A2 (helix J and vicinity of the b sheet 3–3).These results are also original because this is the firstdescription of the role of mutations in the metabolism of aninsecticide ... Eurogentec) in the E. coli strainDH5a.Cytochrome P450 extraction The procedure was the same as described in [39]. At the end of the production, the cultures were chilled on ice (15 min)then centrifuged...
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Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx
... production of inter-leukins and secretion of immunoglobulins on the mucosal surface of the intestine [49,50]. The antibacte-rial defence of the intestinal mucosa depends in parton stimulation of CCK ... euge-nin, indicating that eugenin is indeed acting throughCCK2receptors.To further investigate the interaction of eugenin withCCK2receptors, we investigated the effect of eugeninon lymphocyte ... concerning the sequence from the C-terminal end of the peptide, while the Y+2 fragmentations (shown schematically under-neath the spectrum) provide sequencing data from the N-terminal end of the...
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Tài liệu Báo cáo khoa học: "Using adaptor grammars to identify synergies in the unsupervised acquisition of linguistic structure" docx
... grammar, in which the subtrees are specified in advance, in an adaptor grammar the subtrees, aswell as their probabilities, are learnt from the train-ing data. In order to make parsing and inferencetractable ... parse of thatstring with a PCFG approximation to the adaptorgrammar. This PCFG contains a production for eachadapted subtree in the parses of the other strings in the training corpus. A final ... grammars relax399this independence assumption by in effect learning the probability of the subtrees rooted in a specifiedsubset M of the nonterminals known as the adaptednonterminals.Adaptor grammars...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... contains one gene (AF500)for which no corresponding protein was found in the purified enzyme preparation. The results of the sequenceanalyses of the deduced proteins are given in Table 2. The ... co-ordinate threeheme c molecules. AF503 shows the highest sequencesimilarity to a protein encoded by the dsr locus of A. vinosum, the DsrJ protein. The mature form of the AF503 protein contains ... an intrin sic property of the enzyme. In this case, the signal could, for example, b e g enerated by the co-ordination of a redox-active cysteine residue of the enzyme to a metalcluster. The...
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Từ khóa: Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ